关键词: 宽叶弹簧草, 组织培养, 植株再生

Abstract: The bulbs in the dormant period of Ornithogalum concodianum were used in vitro regeneration system study. The results showed that the medium MS (KH2PO4 doubled)+4.0 mg·L-1 6-BA+0.5 mg·L-1 NAA was suitable for induction of bulbs with 54.2% induction rate; 6-BA was the main factor affecting bulb proliferation, and the medium 2/3MS (KH2PO4 doubled)+4.0 mg·L-1 6-BA+1.0 mg·L-1 NAA+2% sucrose was suitable for bulb proliferation with proliferation coefficient 4.0; the medium 1/2MS (half of large element only)+1.0 mg·L-1 IBA+1.0 mg·L-1 NAA+1.0 g·L-1 AC promoted rooting. The substrate (grass charcoal : garden soil : volcanic rock= 5 : 1 : 1 in volume) was suitable for cultivation, the survival rate was reached 89.6%.

Key words: Ornithogalum concodianum, tissue culture, plant regeneration