Abstract: The stem segments with buds of Emmenopterys henryi were used as explants for studying the effects of 5 plant growth inhibitors at different temperature and light conditions on the in vitro conservation by single-factor experimental method.The results showed that at 25℃,the best CCC,MH,B₉,S3307 and ABA concentrations of Emmenopterys henryi germplasm in vitro conservation were 4 mg/L,4 mg/L,2 mg/L,1 mg/L and 4 mg/L.At 9℃,the best CCC,MH,B₉,S3307 and ABA concentrations were 2 mg/L,1 mg/L,1 mg/L,0.5 mg/L,and 2 mg/L.On light or dark conditions,the best CCC,MH,B₉,S3307 and ABA concentrations were 4 mg/L,4 mg/L,2 mg/L,1 mg/L,and 4 mg/L.Compared with normal temperature conditions,5 plant growth inhibitors had higher survival rate and their best concentrations for Emmenopterys henryi germplasm in vitro conservation generally reduced on low-temperature conditions.And compared with light conditions,5 plant growth inhibitors also had higher survival rate on dark conditions,but their best concentrations were the same as light conditions.The genetic variation of Emmenopterys henryi in vitro conservation plantlets did not appear.

Key words: Emmenopterys henryi, plant growth inhibitor, germplasm, in vitro conservation

摘要： 以香果树带芽茎段为外植体,采用单因子实验法研究了5种植物生长抑制剂在不同温度、不同光照条件下对香果树试管苗离体保存的影响。结果表明,在25℃条件下,香果树种质离体保存的最佳CCC、MH、B₉、S3307和ABA浓度分别为4、4、2、1和4 mg/L;在9℃条件下,香果树种质离体保存的最佳CCC、MH、B₉、S3307和ABA浓度分别为2、1、1、0.5和2 mg/L。在光照和黑暗条件下,香果树种质离体保存的最佳CCC、MH、B₉、S3307和ABA浓度分别为4、4、2、1和4 mg/L。与常温条件相比,低温条件下5种植物生长抑制剂的成活率更高,最佳浓度普遍降低。与光照培养比较,在黑暗条件下5种植物生长抑制剂的成活率也更高,但处理的最佳浓度相同。离体保存后的试管苗没有发生遗传变异。

关键词: 香果树, 植物生长抑制剂, 种质, 离体保存