Abstract: The callus induction and differentiation of Gardenia jasminoides and their multiple buds proliferation and rooting were studied using Gardenia jasminoides stems with a bud as the test material.The results showed:the best medium of callus induction and differentiation of Gardenia jasminoides stems with a bud was MS+KT 1 mg/L+NAA 0.05 mg/L;the best medium of proliferation and rooting of the multiple bud was MS+KT 2 mg/L+NAA 0.05 mg/L.

Key words: Gardenia jasminoides, stems with a bud, callus, multiple buds, proliferation, rooting

摘要： 以栀子带芽茎段为试材,对其愈伤组织诱导分化及丛生芽增殖与生根进行初步研究。结果表明,栀子带芽茎段愈伤组织快速诱导和分化最佳培养基是MS+KT 1 mg/L+NAA 0.05 mg/L;栀子带芽茎段丛生芽增殖和生根最佳培养基是MS+KT 2.0 mg/L+NAA 0.05 mg/L。

关键词: 栀子, 带芽茎段, 愈伤组织, 丛生芽, 增殖, 生根