Abstract: Yunnan wild Dendrobium trigonopus seed plantlets in vitro aseptic germination was used as test material to explore the tissue culture and rapid propagation techniques of D. trigonopus. The results showed that the culture medium of B5 + NAA 0.5 mg·L^-1 + 10% potato juice and B5 + NAA 0.5 mg·L^-1 + 6-BA 0.5 mg·L^-1 + 10% potato juice had a significant effect to seedlings germination and protocorm proliferation of D. trigonopus respectively; the best rooting medium was B5 + NAA 0.5 mg·L^-1+ 10% potato juice + bark powder 2 g·L^-1. Seedlings could be directly transplanted in the matrix of sawdust and wood shaving or sawdust and bark each half when they were simply sterilized, the survival rate exceeded 95% after transplanted 25 days.

Key words: Dendrobium trigonopus, tissue culture, rapid propagation, B5 culture medium

摘要： 以云南野生翅梗石斛Dendrobium trigonopus种子无菌萌发的试管苗为材料，初步探讨翅梗石斛的组织培养和快速繁殖技术。结果表明：B5 + NAA 0.5 mg·L^-1 + 10%马铃薯汁和B5 + NAA 0.5 mg·L^-1 + 6-BA 0.5 mg·L^-1 + 10%马铃薯汁培养基分别对翅梗石斛种子的萌发与原球茎的增殖效果明显；B5 + NAA 0.5 mg·L^-1 + 10%马铃薯汁+云南松树皮粉末2 g·L^-1培养基对翅梗石斛的生根及壮苗效果较好。成苗后进行简单的药剂灭菌，便可直接移栽至锯末与刨花或锯末与树皮比例为1∶1的基质中，25 d后，成活率达95%以上。

关键词: 翅梗石斛, 组织培养, 快速技术, B5培养基