Relationships among 3 Species of Dendrobium Based on RAPD Marker

doi:10.3969/j.issn.1009-7791.2014.02.008

Subtropical Plant Science ›› 2014, Vol. 43 ›› Issue (02): 123-126.DOI: 10.3969/j.issn.1009-7791.2014.02.008

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Relationships among 3 Species of Dendrobium Based on RAPD Marker

ZHANG Yang,CHEN Zhi-kuan,LAI Yu-bo,HUANG Shu-lin,SHAO Hong-wei,

(1.College of Life Sciences and Bio-pharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong China; 2.Guangdong Provincial Key Laboratory of Biotechnology Candidate Drug Research, Guangzhou 510006, Guangdong China)

Received:2013-11-24 Revised:2013-11-24 Online:2014-06-10 Published:2014-06-10
Contact: SHAO Hong-wei

应用RAPD分子标记技术探讨3种石斛属植物的种间关系

张杨,陈志宽,赖育菠,黄树林,邵红伟

（1.广东药学院生命科学与生物制药学院，广东广州 510006；2.广东省生物技术候选药物研究重点实验室, 广东广州 510006）

通讯作者: 邵红伟
作者简介:张杨，本科，生物技术专业。
基金资助:
国家自然科学基金项目(31100664、31300737、81303292）；广东省自然科学基金项目(S2012040007958）；广东药学院师资队伍建设经费

Abstract

Abstract: Three species of Dendrobium, D. nobile, D. officinale and D. devonianum were analyzed using random amplified polymorphic DNA (RAPD) markers. A total of 113 bands were amplified by 10 RAPD primers, among which 106 (93.81%) bands were polymorphic. The data of 113 RAPD bands were used to generate genetic distances and to construct a dendrogram by unweighted pair group method analysis (UPGMA). The results showed that the RAPD approach was efficient in studying genetic background and relationships among these 3 species and could be used to provide abundant molecular data for the medicinal development and utilization of Dendrobium.

Key words: Dendrobium, RAPD, genetic diversity, genetic relationship

摘要： 采用RAPD分子标记技术，分析了金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的种间关系。10个引物产生的113条DNA扩增片段中，106条（93.81%）具有多态性，利用113个RAPD标记，计算遗传距离，利用非加权组平均法建立聚类图。结果表明，RAPD标记技术较好地从分子水平揭示金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的遗传背景、亲缘关系，并为后期在DNA水平上对药用石斛的开发利用提供资料。

关键词: 石斛属, RAPD分析, 遗传多样性, 亲缘关系

CLC Number:

Q943
Q949

ZHANG Yang,CHEN Zhi-kuan,LAI Yu-bo,HUANG Shu-lin,SHAO Hong-wei,. Relationships among 3 Species of Dendrobium Based on RAPD Marker[J]. Subtropical Plant Science, 2014, 43(02): 123-126.

张杨,陈志宽,赖育菠,黄树林,邵红伟. 应用RAPD分子标记技术探讨3种石斛属植物的种间关系[J]. 亚热带植物科学, 2014, 43(02): 123-126.

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Relationships among 3 Species of Dendrobium Based on RAPD Marker

应用RAPD分子标记技术探讨3种石斛属植物的种间关系

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