Abstract: The seeds of Toona ciliata were used as explants to study the tissue culture of T. ciliata. The results showed that the MS＋6-BA 0.5 mg·L^-1＋NAA 0.2 mg·L^-1 media was good for the bud induction of the asepsis seedling, the optimal bud proliferation culture medium was 2/3MS＋6-BA 0.5 mg·L^-1＋NAA 0.2 mg·L^-1＋GA3 1.0 mg·L^-1, and the optimal root induction culture medium was 1/2MS＋IBA 0.5 mg·L^-1, The plantlet was transplanted with the matrix of the peat soil, yellow subsoil and river sand, and the survival rates up to 85%.

Key words: Toona ciliata, seed, tissue culture propagation

摘要： 以红椿种子为外植体材料，对其组织培养技术进行初步研究。结果表明，适宜红椿种子无菌苗芽诱导的培养基为MS＋6-BA 0.5 mg·L^-1＋NAA 0.2 mg·L^-1，适宜的芽继代增殖培养基为2/3MS＋6-BA 0.5 mg·L^-1＋NAA 0.2 mg·L^-1＋GA3 1.0 mg·L^-1，适宜生根培养基为1/2MS＋IBA 0.5 mg·L^-1，以泥炭土、黄心土、河沙按1∶1∶1混合作为基质，移栽成活率达85%以上。

关键词: 红椿, 种子, 组培育苗