Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High 
Temperature and Drought Stress

doi:10.3969/j.issn.1009-7791.2024.05.001

Subtropical Plant Science ›› 2024, Vol. 53 ›› Issue (5): 389-398.DOI: 10.3969/j.issn.1009-7791.2024.05.001

• Research articles • Next Articles

Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High Temperature and Drought Stress

ZHOU Hong-hui^1,2, GE Cong^1,2, HUANG Xiao-bing^1,2, ZHANG Long^1,2, LU Cong-hui^1,2, ZHANG Yu-ping³, WANG Ping^1,2*

(1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 2. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 3 Tianma Citrus Orchard of Yongchun County, Quanzhou 362600, Fujian China)

Received:2024-04-22 Accepted:2024-06-13 Online:2024-10-31 Published:2025-01-25
Contact: WANG Ping

高温干旱胁迫下接种AMF增强芦柑叶片光合与抗氧化能力

周宏蕙^1,2，葛聪^1,2，黄晓冰^1,2，张龙^1,2，卢聪慧^1,2，张宇平³，王平^1,2*

(1. 福建农林大学园艺学院，福建福州 350002；2. 作物遗传育种与综合利用教育部重点实验室，福建福州 350002；3. 福建省永春县天马柑桔场，福建泉州 362600)

通讯作者: 王平
基金资助:
国家现代农业柑橘产业技术体系项目(CARS-26)

Abstract

Abstract: The effects of inoculation with Arbuscular mycorrhizal fungi (AMF) on the photosynthesis and antioxidant capacity in the leaves of Citrus reticulata ‘Ponkan’ under high temperature and drought stress were investigated. This study would provide Ponkan a production measure for resisting high temperature and drought stress. The experimental treatments were designed as follows: (1) Ponkan without AMF inoculation under high temperature and drought stress; (2) Ponkan with AMF inoculation under high temperature and drought stress; (3) Ponkan without AMF treatment under normal environmental conditions (control). In this study, AMF infection rate in the root and phenotype of Ponkan were observed, and the photosynthetic parameters, reactive oxygen species (ROS) content and antioxidant enzyme activities were measured in the leaves. The results showed that the leaves of Ponkan without AMF inoculation were obviously yellowed and wilted under high temperature and drought stress compared with the control. The net photosynthetic rate, stomatal conductance, transpiration rate, water use efficiency, maximum photochemical efficiency, actual photosynthetic efficiency and photosynthetic electron transport efficiency in the leaves of Ponkan without AMF inoculation were significantly decreased under high temperature and drought stress, while the intercellular CO2 concentration was increased compared with those of control. However, the net photosynthetic rate and stomatal conductance were increased, the intercellular CO2 concentration was decreased, and the photosynthetic capacity was enhanced in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. In addition, the contents of O₂^܋, H₂O₂ and malondialdehyde (MDA) in leaves of Ponkan without AMF inoculation were increased while the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) were decreased under high temperature and drought stress compared with those of control. However, the contents of O₂^܋, H₂O₂ and MDA were decreased and the activities of the antioxidant enzymes were increased in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. The results demonstrated that Ponkan growth was inhibited, and the photosynthetic and antioxidant capacities was reduced in the the leaves of Ponkan without AMF inoculation, while the capacities were effectively improved in the leaves of Ponkan with AMF inoculation under high temperature and drought stress. Therefore, we conclude that the higher photosynthetic and ROS scavenging capacities in the leaves of Ponkan with AMF inoculation alleviate the physiological damage of the plants under high temperature and drought stress.

Key words: Ponkan, Arbuscular mycorrhizal fungi (AMF), high temperature and drought stress, photosynthesis, antioxidant enzymes, ROS

摘要： 探究高温干旱胁迫下接种丛枝菌根Arbuscular mycorrhizal 真菌(AMF)对芦柑Citrus reticulata ‘Ponkan’叶片光合能力和抗氧化能力的影响。设置高温干旱胁迫下芦柑接种AMF和不接种两个处理，并以正常环境条件不接种AMF芦柑为对照，对芦柑根系AMF侵染率及其表型进行观察，并测定叶片光合参数指标、活性氧(ROS)和抗氧化酶活性。结果表明，与对照相比，高温干旱胁迫下不接菌芦柑的叶片明显黄化、萎蔫脱落；叶片净光合速率、气孔导度、蒸腾速率降低，胞间CO₂浓度升高；同时，其叶片O₂^܋、H₂O₂、丙二醛(MDA)含量增高，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性下降。与高温干旱胁迫下不接菌芦柑比较，接菌芦柑植株萎蔫速度较慢、萎蔫程度较小；叶片净光合速率、气孔导度、蒸腾速率和水分利用效率、最大光化学效率、实际光合效率明显提升，胞间CO2浓度降低；同时，叶片O₂^܋、H₂O₂ 和MDA含量下降，SOD、CAT、POD和APX活性提升。高温干旱胁迫抑制芦柑生长，降低光合和抗氧化能力；而接种AMF有效增强芦柑叶片光合和抗氧化能力，以及清除ROS能力，缓解高温干旱胁迫对芦柑的生理伤害。

关键词: 芦柑, 丛枝菌根真菌, 高温干旱, 光合作用, 抗氧化, ROS

CLC Number:

Q945

ZHOU Hong-hui, GE Cong, HUANG Xiao-bing, ZHANG Long, LU Cong-hui, ZHANG Yu-ping, WANG Ping. Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High Temperature and Drought Stress[J]. Subtropical Plant Science, 2024, 53(5): 389-398.

周宏蕙, 葛聪, 黄晓冰, 张龙, 卢聪慧, 张宇平, 王平. 高温干旱胁迫下接种AMF增强芦柑叶片光合与抗氧化能力[J]. 亚热带植物科学, 2024, 53(5): 389-398.

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Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High Temperature and Drought Stress

高温干旱胁迫下接种AMF增强芦柑叶片光合与抗氧化能力

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