Effects of Exogenous Gibberellin on Rice Seed Germination and Seedling Growth under Salt Stress

doi:10.3969/j.issn.1009-7791.2024.06.003

Subtropical Plant Science ›› 2024, Vol. 53 ›› Issue (6): 512-519.DOI: 10.3969/j.issn.1009-7791.2024.06.003

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Effects of Exogenous Gibberellin on Rice Seed Germination and Seedling Growth under Salt Stress

QIAO Jiao^*

(Shanxi University of Electronic Science and Technology, Linfen 041000, Shanxi China)

Received:2024-07-18 Accepted:2024-08-19 Online:2024-12-31 Published:2025-03-20
Contact: QIAO Jiao

外源赤霉素对盐胁迫下水稻种子萌发及幼苗生长的影响

乔娇^*

(山西电子科技学院，山西临汾 041000)

通讯作者: 乔娇
基金资助:
山西省高等学校教学改革创新项目(J20231810)；山西省基础研究计划青年科学研究项目(202203021212390)；山西省科技厅，山西省基础研究计划（自由探索类）项目编号202203021212385；山西省高等学校教学改革创新项目（J20231827）

Abstract

Abstract: This experiment investigated the effects of different concentrations of gibberellin (25, 50, 100 mg·L^–1) on rice seed germination and seedling growth under sodium chloride stress. The results showed that during the seed germination stage, exogenous gibberellin enhances the germination of rice seeds under salt stress, with an increase in germination vigor, germination rate, and germination index, and a decrease in relative damage rate. During the growth stage of seedlings, morphological analysis showed that exogenous gibberellin alleviated the inhibition of salt stress on seedling growth, resulting in an increase in plant height, root length, root tip number, and leaf area; Physiological and biochemical analysis showed that exogenous gibberellin improved the activity of superoxide dismutase (SOD) in rice leaves under salt stress, reduced the content of malondialdehyde (MDA) in leaves, increased the content of photosynthetic pigments including chlorophyll a, b and carotenoids, enhanced photosynthesis, increased the effective quantum yield of PSⅡ [Y(Ⅱ)] and the photochemical quenching coefficient (qP), and decreased the non photochemical quenching coefficient (NPQ). In summary, exogenous gibberellin soaking treatment significantly promoted the germination of rice seeds under salt stress, the effects of sodium chloride stress on seedling growth and development was significantly reduced after spraying exogenous gibberellin, and the optimal concentration of gibberellin was 50 mg·L^–1. The research results provide a theoretical basis for optimizing rice cultivation in saline soil.

Key words: gibberellin, salt stress, rice, seed germination, seedling growth

摘要： 通过添加不同浓度赤霉素(GA₃，25、50、100 mg·L^–1)，探究其对氯化钠胁迫下水稻种子萌发和幼苗生长的调控作用。结果表明，在种子萌发阶段，外源赤霉素促进盐胁迫下水稻种子萌发，其发芽势、发芽率、发芽指数均上升，相对伤害率降低。在幼苗生长阶段，形态分析表明，外源赤霉素缓解盐胁迫对幼苗生长的抑制，株高、根长、根尖数及叶面积上升；生理生化分析表明，外源赤霉素提高盐胁迫下水稻叶片超氧化物歧化酶(SOD)活性、降低叶片丙二醛(MDA)含量，提高光合色素含量叶绿素a、b，及类胡萝卜素含量上升，光合作用增强PSⅡ有效量子产量[Y(Ⅱ)]和光化学猝灭系数(qP)上升，非光化学淬灭系数(NPQ)下降。综上所述，外源赤霉素浸种处理可显著促进盐胁迫下水稻种子萌发，喷洒外源赤霉素后氯化钠胁迫对幼苗生长发育的影响明显减弱，最适赤霉素浓度为50 mg·L^–1。该研究结果为优化盐渍地水稻种植提供理论依据。

关键词: 外源赤霉素, 盐胁迫, 水稻, 种子萌发, 幼苗生长

CLC Number:

Q945

QIAO Jiao. Effects of Exogenous Gibberellin on Rice Seed Germination and Seedling Growth under Salt Stress[J]. Subtropical Plant Science, 2024, 53(6): 512-519.

乔娇. 外源赤霉素对盐胁迫下水稻种子萌发及幼苗生长的影响[J]. 亚热带植物科学, 2024, 53(6): 512-519.

References

[1] Romdhane L, Radhouane L, Farooq M, Dal C C, Panozzo A, Vamerali T. Morphological and biochemical changes in maize under drought and salinity stresses in a semi-arid environment [J]. Plant Biosystems – An International Journal Dealing with All Aspects of Plant Biology, 2019, 154(3): 396–404.

[2] Liu J, Wu Y, Dong G, Zhu G L, Zhou G S. Progress of research on the physiology and molecular regulation of sorghum growth under salt stress by gibberellins [J]. International Journal of Molecular Sciences, 2023, 24(7): 6777.

[3] Mu D W, Feng N J, Zheng D F, Zhou H, Liu L, Chen G J, Mu B M. Physiological mechanism of exogenous brassinolide alleviating salt stress injury in rice seedlings [J]. Scientific Reports, 2022, 12(1): 20439.

[4] Sneideris L C, Gavassi M A, Campos M L, Damiao V D, Carvalho R F. Effects of hormonal priming on seed germination of pigeon pea under cadmium stress [J]. Anais da Academia Brasileira de Ciências, 2015, 87(3): 1847–1852.

[5] Shao T, Li L, Wu Y, Chen M, Long X, Shao H, Liu Z, Rengel Z. Balance between salt stress and endogenous hormones influence dry matter accumulation in Jerusalem artichoke [J]. Science of the Total Environment, 2016, 568: 891–898.

[6] Qin X H, Duan Z K. Signal regulation mechanism of plant salt stress [J]. Genomics and Applied Biology, 2019, 38(8): 3706–3713.

[7] Wu M, Zhou J, Li Q, Quan D F, Wang Q W, Gao Y. Auxin-brassinosteroid crosstalk: Regulating rice plant architecture and grain shape [EB/OL]. The Crop Journal, 2024, 12(4): 953–963.

[8] Li N, Wang M Y, Sun J, Su S, Guo S R, Wang J W, Sun H Z. Effects of exogenous 24-epibrassinolide on the growth and photosynthesis of tomato seedlings under low light stress [J]. Acta Botanica Boreali–Occidentalia Sinica, 2013, 33(7): 1395–1402.

[9] 张丽丽, 倪善君, 张战, 赵一洲, 李鑫, 毛艇, 刘研, 刘福才. 外源赤霉素对盐胁迫下水稻种子萌发及幼苗生长的缓释效应[J]. 中国稻米, 2018, 24(2): 42–46.

[10] Khan A, Shaf M, Bakht J, Anwar S. Effect of salinity and seed priming on growth characters of wheat varieties [J]. Sarhad Journal of Agriculture, 2017, 33(3): 435–446.

[11] 苏金强, 谢晓清, 林秋金, 张天祥. 外源赤霉素对盐胁迫下百香果种子萌发及幼苗生长的影响[J]. 广东农业科学, 2023, 50(10): 66–74.

[12] 王康君, 王龙, 顾正中, 李筠, 任立凯, 李强, 孙中伟. 盐胁迫对小麦种子萌发与幼苗生长的影响及外源物质调控效应[J]. 江苏农业科学, 2016, 44(1): 111–115.

[13] 曹艳玲, 卢艳如, 刘海燕, 刘凤兰. 外源SA浸种对盐胁迫下小油菜幼苗地上部生长及生理特性的影响[J]. 北方园艺, 2017(21): 1–6.

[14] 张志良, 李小方. 植物生理学实验指导(第5版) [M]. 北京: 高等教育出版社, 2016: 46–52.

[15] 刘阳, 郭平毅, 原向阳, 高贞攀, 王斌强, 宋惠洁, 黄蕾, 余凯凯. 叶面喷施二甲四氯对‘张杂谷10号’光合特性及产量构成的影响[J]. 西北农业学报, 2016, 25(9): 1311–1318.

[16] 郭美俊, 郭平毅, 原向阳, 高虹, 高贞攀, 冯雷, 王斌强, 宁娜, 余凯凯, 董淑琦. 叶面喷施亚硒酸钠对谷子光合特性及产量构成的影响[J]. 核农学报, 2014, 28(6): 1099–1107.

[17] 张小梅, 王智鑫, 李广录. 水杨酸对铬胁迫下绿豆幼苗生长抑制的缓解效应[J]. 河南农业科学, 2012, 41(11): 52–54.

[18] Teakle N L, Tyerman S D. Mechanisms of Cl-transport contributing to salt tolerance [J]. Plant Cell Environment, 2010, 33(4): 566–589.

[19] 张晓婷, 王雪松, 贾文飞, 徐振彪, 王颖, 吴林. 植物在盐处理下的研究进展[J]. 北方园艺, 2021(6): 137–143.

[20] Yu Z P, Duan X B, Luo L, Dai S J, Ding Z J, Xia G M. How Plant hormones mediate salt stress responses [J]. Trends in Plant Science, 2020, 25(11): 1117–1130.

[21] Wang K J, Wang L, Gu Z Z, Li Y, Ren L K, Li Q, Sun Z W. The effect of salt stress on wheat seed germination and seedling growth and the regulating effect of exogenous substances [J]. Jiangsu Agricultural Sciences, 2016, 44(1):111–115.

[22] 李依民, 梁小燕, 张晗, 高静, 王楠, 徐进, 成世强, 彭亮, 张岗. 赤霉素对NaCl胁迫下大黄种子萌发和幼苗生长的影响[J]. 中草药, 2022, 53(18): 5834–5841.

[23] Zolla G, Heimer Y M, Barak S. Mild salinity stimulates a stress-induced morphogenic response in Arabidopsis thaliana roots [J]. Journal of Experimental Botany, 2010, 61(1): 211–224.

[24] 阿曼姑•艾麦尔艾力, 乃比•阿不来提, 李陈建. 盐胁迫对6个品种饲用甜高粱种子萌发和幼苗生长的影响[J]. 种子, 2024, 43(5): 137–146.

[25] 罗敏, 张荣, 吴翠萍, 袁鹰, 徐连娜, 张培培, 杜锦, 向春阳. 玉米幼苗期耐盐性评价适宜盐浓度的研究[J]. 天津农学院学报, 2015, 22(1): 6–9.

[26] 李寿冰, 赵钰琪, 曹帮华, 袁洪振, 耿颖, 陈炳卓, 张家昌. 盐碱胁迫对2种地被竹叶绿素含量的影响[J]. 世界竹藤通讯, 2022, 20(S1): 35–43.

[27] 徐锴, 金芳. 外源激素对盐胁迫下草莓光合性能的影响[J]. 甘肃农业大学学报, 2006, 41(2): 31–34.

[28] 田敏, 饶龙兵, 李纪元. 植物细胞中的活性氧及其生理作用[J]. 植物生理学通讯, 2015, 41(2): 235–241.

[29] 王改净, 陈亚龙, 赵明君, 徐棒棒, 程紫钦, 高青蕊, 韩赞平. GA₃浸种对玉米种子发芽及生理性状的影响[J]. 种子, 2020, 39(6): 37–42.

[30] Benincasa P, Pace R, Quinet M, Lutts S. Effect of salinity and priming on seedling growth in rapeseed (Brassica napus var. oleifera Del.) [J]. Acta Scientiarum-Agronomy, 2013, 35: 479–486.

[31] 郭佳, 王凤华, 赵峻杰, 蔡金金, 蒋燕. 赤霉素对盐胁迫下樱桃萝卜种子萌发和生长的影响[J]. 蔬菜, 2024(3): 29–35.

[32] 生林山, 韩佳一, 盛雨婷, 陆峻一, 纪元, 张玉梅, 李夕梅. 外源(Z)-3-己烯基乙酸酯缓解小麦盐胁迫的作用机制[J]. 植物生理学报, 2022, 58(7): 1263–1274.

[33] Nimir N E A, Zhou G S, Guo W S, Ma B L, Lu S Y, Wang Y H. Effect of foliar application of GA₃, kinetin, and salicylic acid on ions content, membrane permeability, and photosynthesis under salt stress of sweet sorghum [J]. Canadian Journal of Plant Science, 2017, 97(3): 525–535.

[34] 马洪英, 杨小玲, 仝雅娜, 靳力争, 李响. 4个水果型黄瓜叶片叶绿素荧光特性的比较研究[J]. 天津农业科学, 2019, 25(10): 19–22.

[35] 胡志辉, 梁晓平, 田巧君, 安琪, 汪艳杰. 豇豆品种在盐胁迫下叶片叶绿素荧光特性聚类分析[J]. 种子, 2024, 43(7): 36–44.

[36] Wu Y, Jin X, Liao W, Hu L, Dawuda MM, Zhao X, Tang Z, Gong T, Yu J. 5-Aminolevulinic acid (ALA) alleviated salinity stress in cucumber seedlings by enhancing chlorophyll synthesis pathway [J]. Frontiers in Plant Science, 2018, 9: 635.

[37] 张雪芹, 钟赞华, 赖瑞云, 林建忠, 谢志南. 赤霉素对凤梨释迦生长和叶绿素荧光特性的影响[J]. 中国南方果树, 2020, 49(5): 64–67, 72.

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Effects of Exogenous Gibberellin on Rice Seed Germination and Seedling Growth under Salt Stress

外源赤霉素对盐胁迫下水稻种子萌发及幼苗生长的影响

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