Abstract: To screen out germplasm resources of Forsythia suspensa with high coumarin content and determine the main environmental factors affecting its coumarin metabolism, UPLC-MS/MS was used to analyze the differences of 25 coumarin metabolites in F. suspensa from 10 populations and their correlations with environmental factors. The results showed that the contents of 25 coumarin metabolites were significantly different among 10 populations. Specifically, in both Luanchuan (LC) and Pingshun (PS) populations, the contents of 6 coumarin metabolites were significantly higher than those in other populations. Among them, ostruthin was significantly positively correlated with temperature bio1, bio5, bio8, bio10. Esculin and daphnin were significantly positively correlated with temperature bio4, bio7, bio2. Dimethyllimettin was significantly positively correlated with temperature bio9, bio11. Fraxetin glucose glucoside was significantly negatively correlated with temperature bio6, bio9, bio11. Dimethyllimettin, coriandrin, 4',5'-dihydropsoralen, 5,8-dihydroxypsoralen, 6-hydroxy-4-methylcoumarin were significantly positively correlated with precipitation bio17, bio19. In conclusion, the LC and PS populations exhibited high levels of coumarin, a bioactive component in F. suspensa. This could be attributed to local adaptation to environmental heterogeneity, particularly in terms of temperature and precipitation, rather than altitude. The research findings hold reference value for the selection and breeding of superior germplasm resources of F. suspensa, as well as for its introduction, cultivation, and production management.

Key words: Forsythia suspense, coumarin metabolism, environmental heterogeneity, association analysis

摘要： 为筛选出连翘Forsythia suspensa药效成分香豆素含量高的种质资源，并确定影响其香豆素代谢的主要环境因子，采用UPLC-MS/MS分析10个种源连翘的25种香豆素代谢物差异及其与环境因子相关性。结果表明，不同种源连翘25种香豆素代谢物差异显著，栾川(LC)、平顺(PS)种源均有6种香豆素代谢物含量显著高于其他居群。其中，王草质与年均温、最暖月最高温、最湿季度平均温度、最暖季度平均温度等温度指标呈显著正相关；秦皮甲素、6,7-二羟基香豆素-6-O-葡萄糖苷和瑞香苷与温度季节性变化标准差、年均温变化范围、昼夜温差月均值等温度指标呈显著正相关；二甲基柠檬油素与最干季度平均温度、最冷季度平均温度等温度指标呈显著正相关；梣皮素二葡萄糖苷与最冷月最低温、最干季度平均温度、最冷季度平均温度等呈显著负相关；二甲基柠檬油素、芫荽异香豆精、4',5'-二氢补骨脂素、5,8-二羟基补骨脂素、6-羟基-4-甲基香豆素与最干季度降水量、最冷季度降水量呈显著正相关。综上，LC和PS种源为连翘香豆素含量高的居群，可能是对温度和降水而非海拔的环境异质性的局域适应。研究结果对选育连翘优良种质资源及引种栽培和生产管理有参考价值。

关键词: 连翘, 香豆素代谢, 环境异质性, 关联分析