Abstract: The aseptic seedling results of Cymbidium scinense were observed that when they were cultured on the media supplemented without any cytokinin, seeds can sprout but only protocorm and rhizome came into being, and they cannot differentiate further into seedlings. Only on the MS or Knudson C media containing different hormones can they induce shoot initiation. The medium added with 0.5~1.0mg/L 6-BA and 0.1mg/L NAA gave the best results. The MS medium added with 2.0mg/L BA and 0.4mg/L NAA can accelerate greatly shoot differentiation rate, when rhizome was transferred onto the liquid proliferation medium added with the same hormones and cultured at the stage of vibration. The medium added with 0.5% activated carbon had obvious synergy effect for shoot differentiation. MS medium added with 0.2mg/L NAA gave the best result in seedling rooting.

Key words: Cymbidium scinense, protocorm, rhizome, root hair, bud differentiation

摘要： 墨兰的无菌播种结果发现,在不添加细胞分裂素的培养基上,种子可以发芽,但只有原球茎和根状茎产生;不可能进一步分化成苗,只有在含有不同激素成分的MS或KnudsonC培养基上,才有可能诱导芽的分化,其中以附加6-BA 0.5-1.0mg/L+NAA 0.1mg/L诱导效果最佳,在附加6-BA 2.0mg/L+NAA 0.4mg/L的MS培养基中,能加速芽的增殖,根状茎转入含有相同激素成分的液体增殖培养基中振荡培养,可大大提高芽的分化速率。添加0.5%活性炭对芽的分化有明显增效作用。在附加NAA 0.2mg/L的MS培养基中;幼苗的生根效果最佳。

关键词: 墨兰, 原球茎, 根状茎, 根毛, 芽分化