Abstract: The haustorial bud explants from Musella lasiocarpa were cultured on MS medium supplemented with 6-BA and IBA at different concentration to induce regeneration plantlets. The results indicated that the haustorial buds can be induced on medium of MS+6-BA 1.0mg/L+IBA 0.5mg/L after 30 days. Proliferation medium was MS+6-BA 0.3mg/L+IBA 0.1mg/L. The reproduction coefficient was more than 2.83. The optimal medium for rooting was MS+NAA 1.0mg/L and the rooting percentage was 100%. The transplant survival percent was more than 95%.

Key words: Musella lasiocarpa, tissue culture, rapid propagation

摘要： 以地涌金莲吸芽为外植体,在MS+6-BA 1.0mg/L+IBA 0.5mg/L培养基上培养30d后产生幼芽;丛芽增殖培养基为MS+6-BA0.3mg/L+IBA0.1mg/L,增殖系数达2.83以上;生根诱导最佳培养基为MS+NAA1.0mg/L,生根率达100%。移栽成活率95%以上。

关键词: 地涌金莲, 组织培养, 快速繁殖