Abstract: The effects of several factors on dedifferentiation and redifferentiation of Pueraria lobata earthnut were studied. The results showed that the best disinfection way of Pueraria lobata stems with a bud was firstly treated with 70% ethyl alcohol for 30 seconds then treated with 0.1% HgCl₂ for 15 minutes, and the optimum regeneration culture medium was MS+NAA 0.5mg/L+6-BA 5.0mg/L. Liquid culture with sucrose 30g/L, pH 6.0, cultured in light at 25℃ gave a best result for high frequency plant regeneration of Pueraria lobata.

Key words: Pueraria lobata, high frequency plant regeneration, stem with a bud, tissue culture

摘要： 对野葛高频植株再生体系相关因子进行优化,结果表明,野葛带芽茎段再生体系的最佳消毒方式为70%酒精处理30s后用0.1% HgCl₂处理15min;最佳培养基为MS+NAA 0.5mg/L+6-BA 5.0mg/L;最佳培养条件为:蔗糖浓度30g/L,pH 6.0,液体培养,培养温度25℃,光照培养。

关键词: 野葛, 高频植株再生体系, 带芽茎段, 组织培养