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10 March 2008, Volume 37 Issue 01 Previous Issue    Next Issue

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Insect Herbivory Patterns on Leaves of 17 Plant Species in the Evergreen Broad-leaved Forests of Mt. Meihuashan, Fujian Province CHEN Ling, CAI Yong-li, LI Kai, WANG Hong-wei, TIAN Yu-peng, YE Qian 2008, 37(01): 1-5.  Abstract ( )   PDF (887KB) ( )   Herbivory pattern on leaves is a useful way to evaluate insect species and prevent insect pest. It was investigated the herbivory patterns and intensity on the leaves of 17 evergreen plant species in subtropical evergreen broad-leaved forests which grown on about 450 meters and 1 200 meters Mt. Meihuashan, Fujian Province. The results were as follows:(1) A total of 15 herbivory patterns were found, and for each tree species, the herbivory patterns ranged from 8 to 14. The frequency of each herbivory pattern on leaves of 17 plant species varied from 0.5% to 28.7%. The highest frequency was Edge Defoliation (ED) (37.01%), while that of Middle Vein Defoliation (MVD) was the lowest (0.02%). (2) The herbivory intensity on the leaves of Schima superba was the highest (9.60%), that of Castanopsis fargesii was next (6.61%), and while that of Camellia Oleifera was the lowest (0.34%). (3) Correlation analysis showed that there were significant positive correlations in six pairs of herbivory patterns and significant negative correlations in two pairs. (4) The investigation of four plant species, Castanopsis eyrei, Schima superba, Castanopsis fargesii, Camellia fraternal, growing separately in 1 200 meters and 450 meters mountain, showed that the herbivory intensity on leaves of the four plant species in 1200 meters elevation was higher than that in 450 meters elevation. And the greatest range of herbivory intensity in four plant species was Schima superba, from 13.81% to 2.93%. Related Articles | Metrics

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Study on Genetic Transformation of Pr Gene to Gerbera hybrida Through Agrobacterium YE Wen-biao 2008, 37(01): 6-11.  Abstract ( )   PDF (2299KB) ( )   We transformed the Pr gene to Gerbera hybrida via agrobacterium-mediated transformation, established an efficient system of genetic transformation for agrobacterium and obtained regeneration plantlets. PCR and GUS analysis confirmed the integration and expression of the transgene. Related Articles | Metrics

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ITS Sequence Analysis of Nuclear Ribosomal DNA of Prunus mume in Fujian Province ZHAI Huan-chen, SONG Ya-na, ZHENG Wei-wen 2008, 37(01): 12-16.  Abstract ( )   PDF (980KB) ( )   To study the ITS sequence variation of Prunus mume in Fujian province from different population, the rDNA ITS regions of P. mume varieties were amplified and cloned and sequenced, and sequence data was analyzed by DNAMAN and MEGA3.1. The complete sequence of ITS and 5.8S rDNA of 18 different P. mume population were obtained. The sequences of ITS1, 5.8S and ITS2 are 223~224 bp, 164 bp and 241~246 bp, respectively, 5.8S is more conservative than ITS. Phylogenetic tree based on ITS sequences data was conducted by UPGMA method, which shows the variation at the level of molecular biology. It provided the basis for variety identification of P. mume. And 14 rDNA ITS sequences of P. mume were deposited in GenBank under number EF523482-EF523493, EF529435 and EF529436. Related Articles | Metrics

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Effects of Spraying KH2PO4 During Period of Fruits Growth on Storage of Litchi chinensis cv. Sanyuehong Fruits ZHOU Kai-bing, XU Yuan-feng 2008, 37(01): 17-20,24.  Abstract ( )   PDF (936KB) ( )   It was conducted by spraying the solution with the concentration 0.2% and 0% of KH2PO4 (KP) every 7 days during the period from fruitlet to fruit pigmentation to study the effects of P and K on the storage of fruits. All the fruit samples were stored by mobile atmosphere control storage under a low temperature (4℃). The results were as follows. (1) During the period of storage, there was non-signifcant difference in the loss rate of weight between the treatment KP and CK before 17th day. The tendencies of the changes in the TSS in flesh of the treatment KP and CK were similar. Their curves were S-shaped. The general tendencies of the changes in the TA in flesh of the treatment KP and CK were down, and the curves were alike. The trend of the changes in the content of VitC in flesh of the treatment KP and CK were similar with mono-peak curves, and the peaks happened on 3rd day within the storage period. The tendencies of the changes in the contents of anthocyanidin in peel of the treatment KP and CK were similar with concavity-shaped curves. (2) During the period of storage, the content of soluble protein in peel is extremely higher than that in flesh. The changes in the contents of soluble protein in flesh had a significant difference from that in peel. The tendencies of the changes in the contents of soluble protein in peel of the treatment KP and CK were in common before 10th day, and from then on, the tendencies were opposite to each other. (3) During the period of storage, the activity of POD was remarkably higher in peel than in flesh. The treatment KP and CK had similar tendencies of the changes in the POD activity in flesh 3 days later and in peel before 17th day. (4) During the period of storage, the CAT activity in flesh was extremely higher than that in peel. The tendencies of the changes in CAT activity in flesh and in peel were similar to each other between the treatment KP and CK, and the two curves had mono-peak. Related Articles | Metrics

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Effect of Low Potassium Stress on Root Growth and Endogenous Phytohormones Contents of Different Rice Genotypes at Seedling Stage KU Wen-zhen, PENG Ke-qin, XIAO Lang-tao, ZHANG Xue-qin, SHEN Zhi-jin, HUANG Xin 2008, 37(01): 21-24.  Abstract ( )   PDF (853KB) ( )   Two low-K tolerant rice genotypes named N18 and N19, and two low-K sensitive rice genotypes named N27 and N28 were used in this experiment to investigate the effects of low potassium stress on the root growth and contents of endogenous phytohormone in roots of them at seedling stage. The results showed that under low potassium stress, root length and dry weight of all genotypes were decreased, which were longer and higher significantly of N18 and N19 than those of N27 and N28. Furthermore, low potassium stress increased the ratio of root/shoot, but the differences in it were inconspicuous among the four genotypes tested. Under low potassium stress, contents of IAA, GA1 and ZR in roots of all genotypes were decreased, which were higher in N18 and N19 than those in N27 and N28, while contents of ABA in roots of all genotypes were increased. Furthermore, under low potassium stress, the ratios of IAA/ABA, ZR/ABA and GA1/ABA in roots of all genotypes were reduced, which were higher in N18 and N19 than those in N27 and N28. Related Articles | Metrics

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Anatomy Study on Protocorm-like Bodies Formation and Development of Cymbidium hybridium WANG Yan-ping, WANG Guang-dong, WU Zhen 2008, 37(01): 25-28.  Abstract ( )   PDF (2820KB) ( )   Anatomy study on the protocorm-like bodies formation and development of Cymbidium hybridium was conducted. The results showed that callus formed firstly from shoot tip of explant, then differentiated into protocorm-like bodies. In the process of protocorm-like bodies proliferation, variegated forms of pre-protocorms formed around one protocorm-like body which developed into a plantlet at the same time. As for the controversial question of protocorm-like bodies derivation, our results showed that the origin of protocorm-like bodies was a specific regeneration process that have both characteristics of the pathways of somatic embryogenesis and organogenesis; the exact regeneration pathway differed according to genotypes and culture conditions. Related Articles | Metrics

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Optimization of Factors Related to High Frequency Plant Regeneration of Pueraria lobata HONG Sen-rong, YIN Ming-hua, SHAO Xing-hua 2008, 37(01): 29-32.  Abstract ( )   PDF (879KB) ( )   The effects of several factors on dedifferentiation and redifferentiation of Pueraria lobata earthnut were studied. The results showed that the best disinfection way of Pueraria lobata stems with a bud was firstly treated with 70% ethyl alcohol for 30 seconds then treated with 0.1% HgCl2 for 15 minutes, and the optimum regeneration culture medium was MS+NAA 0.5mg/L+6-BA 5.0mg/L. Liquid culture with sucrose 30g/L, pH 6.0, cultured in light at 25℃ gave a best result for high frequency plant regeneration of Pueraria lobata. Related Articles | Metrics

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Research on Shoot-tip Culture of Canna×generalis WANG Dan, LEI Jiang-li, WU Yan-min, LÜ Hui, YU Ji-hua 2008, 37(01): 33-36.  Abstract ( )   PDF (2070KB) ( )   The paper mainly studied on tissue culture of Canna×generalis with the stem tips as explants. The results showed that the bud inoculation medium was MS+6-BA 8.0mg/L+ TDZ 0.03mg/L; the best of clump shoot induction and differentiation medium was MS+6-BA 8.0mg/L+TDZ 0.03mg/L+NAA 0.1mg/L; using MS+6-BA 3.0mg/L+TDZ 0.03mg/L+NAA 0.1mg/L as proliferation medium, an optimal proliferation rate was obtained. When the two kinds of medium used alternatively, the effect was better. The optimum rooting medium was MS+6-BA 1.0mg/L+NAA 0.5mg/L, the rate of rooting could reach 66.67%, and cultured in this medium, the plant grew well and easy to survival. Related Articles | Metrics

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Study on Plantlet Differentiation and Growth of Oncidium WEI Cui-hua, XIE Yu, CHEN Qin 2008, 37(01): 37-40.  Abstract ( )   PDF (885KB) ( )   The test for passing domestication culture of Oncidium adventitious buds from PLBs showed that taking the modified 1 medium as basic medium supplemented with 100g/L coconut milk for organic additive and no plant-growth regulator were favorable for the PLBs regeneration and could promote the adventitious buds to grow healthy and good uniformity. According to the test of culture medium, organic additive, the amount of activated charcoal, illumination intensity, container and the altitude of the plantlet to fixing into the container, esc, all which relating to the culture elements of PLBs rhizogenesis plantlet strengthening were screened. Results showed that, all plantlets growing from modified 1 medium with 100g/L banana mud, 1g/L activated charcoal, no plant-growth regulator, under the strong light (2 000~3 000 lx), which promoting the growth of plantlets had better performance in each growing index, and they were favorable for the survival of the later domestication. Results showed that also the plants growing in the triangular flask had highest inoculation rate, the lowest contamination rate and better growth index. In the altitude 3 cm of the plantlet to fixing into the container, the efficiency of factory micropropagation of Oncidium was improved. Related Articles | Metrics

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A Study on Cultivar Pre-identification Technology of Drought Resistance in Flue-cured Tobacco LI Yong-liang, ZHOU Ji-heng, LI Yong-ping, YANG Hong-qi, LU Xiu-ping, MA Wen-guang 2008, 37(01): 41-44.  Abstract ( )   PDF (840KB) ( )   The technology of tissue culture on five flue-cured tobacco varieties (Hong da, K326, NC82, G28, RG11) which were screened and evaluated for drought resistance using the average of subordinate function value based on morphological indexes and physiological indexes closely related to drought resistance were studied. The results showed that plant fresh weight, taproot length, total length of lateral root, root system vigor, chlorophyll content and protoplasmic membrane penetrable of a tobacco variety under normal conditions differed remarkably from those of the same variety under osmotic stress treatment, and fresh weight, total length of lateral root and permeability of plasma membrane were the sensitive indexes of drought resistance of flue-cured tobacco. In addition, the study indicated that drought resistance in different tobacco varieties was highly variable, and the descending order of drought resistance was NC82, RG11, Hong da, G28, K326. Meanwhile,this techniques could be used in screening and evaluation of varietal resistance of tobacco germplasm resources Related Articles | Metrics

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Drought Tolerance Index of Four Shrub Ground Cover Plants WU Lin-sen, FAN Xiao-mei, TANG Xun-yuan 2008, 37(01): 45-47.  Abstract ( )   PDF (811KB) ( )   The drought tolerance of four species of shrub ground cover plants was studied. The results showed that the descend order of drought tolerance was Distylium buxifolium, Pyracantha fortuneana, Loropetalum chinense var. rubrum, Gardenia jasminoides var. radicans. Distylium buxifolium could survive under drought stress and be used as important color plant in city landscaping. Related Articles | Metrics

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Ultrasonic Degradation of Extracellular Polysaccharide from Porphyridium cruentum LIU Mei, CHEN Bi-lian, CHEN Wei-ping 2008, 37(01): 48-50.  Abstract ( )   PDF (830KB) ( )   The effect of ultrasonic degradation of extracellular polysaccharide from Porphyridium cruentum was studied by determining the viscosity of solution. Uniform design method was used to optimize the parameter of ultrasonic, i.e. amplitude, irradiation time and pulse on the degradation of extracellular polysaccharide from Porphyridium cruentum. The optimal conditions for the viscosity were as follows:amplitude 39%, irradiation time 245s and pulse 9.5s. Under the optimal condition, the experimental value of the viscosity of the extracellular polysaccharide achieved to 2.98 mm2/s, compared to the calculated value, the error was 0%. Quadratic polynomial of DPS software was used to analyze and fit the result of the experiment, and the significance of model and regression coefficients was tested in order to establish a regression equation aimed at the viscosity of extracellular polysaccharide. Related Articles | Metrics

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Antimicrobial Activities of Polysaccharide Extracts from Flos lonicerae and Ilex kudingcha LIN Xiong-ping, CHEN Xiao-qing, SU Yu-cai, CHEN Hui-bin 2008, 37(01): 51-53.  Abstract ( )   PDF (830KB) ( )   The crude polysaccharides were extracted from flos lonicerae and Ilex kudingcha by hot water. These crude extracts were tested against seven food spoilages using the agar disc diffusion method. The result showed that polysaccharide extracts from flos lonicerae and Ilex kudingcha have varying amount of activities against bacteria and fungi, and the antibacterial activities are stronger than antifungal activities. The minimum inhibition concentrations (MIC) of polysaccharide extracts of Ilex kudingcha against Staphylococcus aureus was 5mg/ml. The results provided scientific base for exploiting this peculiar resources. Related Articles | Metrics

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Weed Control Effect of Herbicide Metolach to Tobacco Fields GU Mei-ling, GUO Fang-jun, TAN Jun 2008, 37(01): 54-56.  Abstract ( )   PDF (799KB) ( )   In order to study the effect of herbicide Metolach in tobacco fields, the experiments of different periods and different concentration of application were studied in 2006. Compared with TA (Treatment A:applying Metolach 7 days before transplanting tobacco seedling, film mulching), the weed control effect of TB (Treatment B:applying Metolach 25 days after transplanting tobacco seedling, uncovering film and hilling up) was more significant. Among the different concentration,Ta (Treatment a:spraying at a dilution of 400+)and Tb (Treatment b:spraying at a dilution of 550+) gave better results than Tc (Treatment c:spraying at a dilution of 800+). So it's recommended to apply herbicide Metolach at a dilution of 550+ when uncovering film and hilling up (25 days after transplanting) for weed control of tobacco fields. Related Articles | Metrics

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Identification and Conservation of Familiar ‘Coptis’ in Gaoligong Mountain, Yunnan Province SHI Xiao-chun, WANG Jian-yun, MU Qi-ai 2008, 37(01): 57-59.  Abstract ( )   PDF (829KB) ( )   In this paper, the morphological identification, ecology and utilization of local plants, which usually called ‘coptis’, including 2 families 6 genera in Gaoligong Mountain area, were discussed. And some useful measures of utilization and conservation were put forward. Related Articles | Metrics

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Germplasm Resources and Development of Wild Vacciniam spp. in Guizhou Province NIE Fei, AN Ming-tai 2008, 37(01): 60-62.  Abstract ( )   PDF (874KB) ( )   According to the field investigation and data documents, the species (varieties), distribution, habitat and utilization of wild Vacciniam spp. in Guizhou Province were described in this paper. Some suggestions for development of the wild resources were also proposed. Related Articles | Metrics

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Embryo in Vitro Culture and Plantlet Regeneration of Cocos nucifera WU Yi, WU Yao-ting, MA Zi-long, ZHOU Shi-ye 2008, 37(01): 63-64.  Abstract ( )   PDF (1047KB) ( )   Related Articles | Metrics

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Propagation of Amorphophallus konjac Callus in Bioreactor WU Jin-ping, XIANG Fa-yun, SONG Zhi-hong, GU Yu-cheng, HU Zhong-li 2008, 37(01): 65-66.  Abstract ( )   PDF (799KB) ( )   Related Articles | Metrics

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ABA Regulation of Lateral Root Development in Plants GUO Dong-liang, LI Ling 2008, 37(01): 67-69,75.  Abstract ( )   PDF (875KB) ( )   It is summarized the advance of lateral root development regulated by ABA. ABA inhibits the expression of some gene involving in lateral root initiation and activation for lateral meristem during lateral root formation. Lateral root development is inhibited by nitrate in growth medium and drought stress, while ABA alleviates the inhibition. The mechanism of ABA and auxin interacting during the formation of lateral root is complex. Related Articles | Metrics

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Research Progress of Psammophilous Vegetation on Coasts in China LUO Tao, YANG Xiao-bo, HUANG Yun-feng, YE Fan, DANG Jin-ling 2008, 37(01): 70-75.  Abstract ( )   PDF (913KB) ( )   The article reviews the present research situation of the psammophilous vegetation on coasts in china from six respects, including the distribution, habitats, composition, succession, adaptability and function. Related Articles | Metrics

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Recent Advances in Biosynthesis and Regulation of Legume Galactomannan WEI Xiao-chun, ZHENG Qun, LIU Jun-jie 2008, 37(01): 76-81.  Abstract ( )   PDF (940KB) ( )   The hypotheses of biosynthesis pathway and regulation of legume galactomannan are reviewed in this paper. Based on these, the references for vitro-synthesis, regulation and the site of biosynthesis of legume galactpmannan are provided. The perspective of the development of galactomannan is also involved. Related Articles | Metrics

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Research Advances in Ficus microcarpa HAN Na, PAN Dong-ming 2008, 37(01): 82-84.  Abstract ( )   PDF (808KB) ( )   The status of cultivation techniques, diseases and insect pests management, post harvest storage, transport and export of Ficus microcarpa are summarized, and some suggestions for further study are put forward. Related Articles | Metrics