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Subtropical Plant Science

Subtropical Plant Science ›› 2008, Vol. 37 ›› Issue (01): 33-36.

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Research on Shoot-tip Culture of Canna×generalis

WANG Dan1,2, LEI Jiang-li2, WU Yan-min3, LÜ Hui2, YU Ji-hua1   

  1. 1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu China;
    2. Shenzhen Institute of Landscape Gardening, Shenzhen 518003, Guangdong China;
    3. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2007-10-25 Online:2008-03-10 Published:2008-03-10

大花美人蕉茎尖组织培养技术研究

王丹1,2,雷江丽2,吴燕民3,吕慧2,郁继华1   

  1. 1. 甘肃农业大学农学院, 甘肃 兰州 730070;
    2. 深圳市园林科学研究所, 广东 深圳 518003;
    3. 中国农业科学院生物技术研究所, 北京 100081
  • 作者简介:王丹(1982-),女,辽宁本溪人,硕士研究生,从事植物生物技术研究。
  • 基金资助:

    深圳市科技和信息局基金资助项目

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Abstract: The paper mainly studied on tissue culture of Canna×generalis with the stem tips as explants. The results showed that the bud inoculation medium was MS+6-BA 8.0mg/L+ TDZ 0.03mg/L; the best of clump shoot induction and differentiation medium was MS+6-BA 8.0mg/L+TDZ 0.03mg/L+NAA 0.1mg/L; using MS+6-BA 3.0mg/L+TDZ 0.03mg/L+NAA 0.1mg/L as proliferation medium, an optimal proliferation rate was obtained. When the two kinds of medium used alternatively, the effect was better. The optimum rooting medium was MS+6-BA 1.0mg/L+NAA 0.5mg/L, the rate of rooting could reach 66.67%, and cultured in this medium, the plant grew well and easy to survival.

Key words: Canna×, generalis, shoot-tip, tissue culture

摘要： 以大花美人蕉(Canna×generalis)根茎茎尖为外植体进行组织培养技术研究,筛选出芽诱导适宜的培养基为MS+6-BA 8.0mg/L (单位下同)+TDZ 0.03;MS+6-BA 8.0+TDZ 0.03+NAA 0.1培养基能较好地诱导分化出丛生芽,继代增殖培养中与MS+6-BA 3.0+TDZ 0.03+NAA 0.1培养基交替使用可减少畸形芽,增殖系数达1.67;适宜的生根培养基为MS+6-BA 1.0+NAA 0.5,生根率达66.67%,且植株生长健壮,移栽易成活。

关键词: 大花美人蕉, 茎尖, 组织培养

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