Abstract: The effects of Mn2+-Fe2+ combined stress on physiological indexes of Rivina humilis was studied by the method of nutrient solution culture. The results showed that both the chlorophyll content and the soluble sugar content were significantly higher than those of the control and reached the maximum when R. humilis were stressed by 6 mmol/L Mn²⁺ and 0.5 mmol/L Fe²⁺. At the same time, the MDA content was extremely significantly lower than that of the control and got to the minimum. Additionally, the combined treatment of Mn²⁺-Fe²⁺ also improved the activities of SOD, POD and CAT. In a word, the suitable combined concentrations of Mn²⁺ and Fe²⁺could alleviate the toxicity of single metal to some extent, and enhanced the tolerance of R. humilis to the ferromanganese stress.

Key words: Rivina humilis, Mn2+-Fe2+combined stress, physiological indexes

摘要： 以胭脂草(Rivina humilis)为材料，采用溶液培养法，研究系列浓度的锰铁复合胁迫对其若干逆境生理指标的影响。结果表明，当锰铁复合胁迫浓度为6 mmol/L Mn²⁺＋0.5 mmol/L Fe²⁺时，叶绿素含量和可溶性糖含量均极显著高于对照，并达最大值；与此同时，MDA含量极显著降低，并达呈最小值。该组合处理能维持胭脂草较高水平的保护酶（SOD、POD、CAT）活性。由此说明，适宜浓度的锰铁复合处理，能在一定程度上缓解单一金属的毒害作用，提高胭脂草对锰铁复合胁迫环境的耐受性。

关键词: 胭脂草, Mn²⁺-Fe²⁺复合胁迫, 生理指标