Abstract: To investigate the antioxidant activity of total flavonoids extracts from Gnaphalium affine, the raw material of “Haocaibaba” in western Hunan, the effects of total flavonoids extracts from the plant on DPPH, ABTS free radical scavenging, reducing ability and inhibition β-Carotene fading were determined in this study. The results showed that the concentration flavonoids in the mother liquor of the total flavonoids of Gnaphalium. affine mother solution was 7.01 mg·mL–1; the total flavonoids extract had good scavenging ability on DPPH and ABTS free radicals, and its the semi-inhibitory concentration (IC50) was 16.30 mg·L–1 and 30.16 mg·L–1, respectively. The results showed that the total flavonoids extracts of Gnaphalium affine had good scavenging ability to DPPH free radicals and ABTS free radicals, and its half-inhibition concentrations (IC50) were 16.30 mg·L-1 and 30.16 mg·L-1, respectively. The time of decreasing relative absorbance of carotene to 50% was extended to 67.49 min, which also had better effects in reducing ability, delaying carotene fading and inhibiting lipid peroxidation. The total flavonoids of Gnaphalium affine had good in vitro antioxidant activity, which could provide a basis for further promotion and vigorous development of Gnaphalium G. affine as a high-quality edible plant resource.

Key words: western Hunan, “Haocaibaba”, Gnaphalium affine, total flavonoids, antioxidant activity

摘要： 为了解湘西特色食品“蒿菜粑粑”原料植物鼠麴草(Gnaphalium affine)总黄酮提取物体外抗氧化能力，采用DPPH、ABTS自由基清除实验，还原力实验和抑制β-胡萝卜素褪色实验等方法，测定鼠麴草总黄酮抗氧化活性。结果显示，鼠麴草总黄酮母液中总黄酮浓度为7.01 mg·mL–1mg/mL；总黄酮提取物对DPPH、ABTS自由基有较好的清除能力，其半数抑制浓度(IC50)分别为16.30 mg·L–1、30.16 mg·L–1，将胡萝卜素相对吸光度降为50%的时间延长至67.49 min，在还原能力、延缓胡萝卜素褪色和抑制脂质过氧化上也有较好效果。鼠麴草总黄酮提取物具有良好的体外抗氧化活性，可作为优质食用植物资源进一步开发与推广。

关键词: 湘西, 蒿菜粑粑, 鼠麴草, 总黄酮, 抗氧化活性