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Subtropical Plant Science

Subtropical Plant Science ›› 2004, Vol. 33 ›› Issue (04): 29-31.

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Callus Induction of Phalaenopsis

WU Cheng-hou1,2, YE Xiu-lin2, LIANG Cheng-ye2   

  1. 1. Department of Biology, Zhangzhou Normal College, Zhangzhou 363000, Fujian China;
    2. South China Institute of Botany, Academic Sciences of China, Guangzhou 510650, Guangdong China
  • Received:2004-06-01 Online:2004-11-10 Published:2004-11-10

蝴蝶兰愈伤组织诱导研究

伍成厚1,2,叶秀粦2,梁承邺2   

  1. 1. 漳州师范学院 生物系, 福建 漳州 363000;
    2. 中国科学院 华南植物研究所, 广东 广州 510650
  • 作者简介:伍成厚(1968-),男,湖南邵阳人,讲师,博士,从事园林植物遗传育种与生物技术研究。
  • 基金资助:

    广东省重大科技专项(2003A2010401)和福建省青年科技人才创新项目基金(2004J052)资助

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Abstract: The experimental results of callus induction of Phalaenopsis showed that ovaries of Phalaenopsis after 30 days of pollination were the optimal materials for callus induction. When the ovary slices were inoculated on 1/4 MS medium supplemented with 2,4-D 1.0mg/L, 6-BA 0.1mg/L and 3.0% sucrose, the induction rate of callus was 90.0%.

Key words: Phalaenopsis, ovary, callus, induction

摘要： 对蝴蝶兰愈伤组织诱导试验结果表明,授粉后30d的蝴蝶兰子房适宜诱导愈伤组织,在1/4MS+2,4-D1.0mg/L+6-BA 0.1mg/L+蔗糖3.0%培养基上,蝴蝶兰子房切段愈伤组织的诱导率达到90.0%。

关键词: 蝴蝶兰, 子房, 愈伤组织, 诱导

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