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Extraction and Determination of Total Flavonoids from Root, Stem and Leaf of Suaeda australis
HUANG Xiao-kun, ZHU Jia-yuan, HUANG Xiao-dong, MIU Cui-yan, CHEN Jie-fang
2006, 35(04):
35-38.
The effects on the flavonoids content in roots, stem and leaves of Suaeda australis of different concentration ethanol, ratio of material to liquid, extraction temperature and time were studied by the orthogonal experiment.Extracted by the optimum extraction conditions, purified by polycaprolactan, produced color with Al(NO3)3, the flavonoids content was determined.Results showed that the optimum conditions of extraction were as follows:from root, extracted by 50:1, 45% ethanol at 80℃ for 30min;from stem, extracted by 30:1, 60% ethanol at 80℃ for 50min;from leaf, extracted by 30:1, 75% ethanol at 80℃ for 30min.The flavonoids content in root, stem and leaf of Suaeda australis was 1.50%, 0.94%, 6.35%, respectively.
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