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30 June 2018, Volume 47 Issue 02 Previous Issue    Next Issue
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Plant physiology, biochemistry and molecular biology
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Plant physiology, biochemistry and molecular biology

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Core Collection Construction of Endangered Plant Tetracentron sinense Based on ISSR Molecular Markers DUAN Fan,ZHANG Huan,LI Shan,TIAN Zhong-qiong,GAN Xiao-hong 2018, 47(02): 101-106.  DOI: 10.3969/j.issn.1009-7791.2018.02.001 Abstract ( )   PDF (5117KB) ( )   Taking 174 individuals of Tetracentron sinense 26 populations as materials, 3 sampling strategies (random sampling strategy, allele preferred sampling strategy and deviation sampling strategy) were used to construct its core collection based on ISSR markers. Genetic diversity indexes were used to determine the most suiconstruction method. 180 bands were amplified14 ISSR primers, and the observed number of alleles(Na), effective number of alleles(Ne), Nei’s genetic diversity(H) and Shannon information index(I) of original germplasm were 1.6944, 1.3912, 0.2321, 0.3509 respectively, indicating the abundant genetic diversity of T. sinense was low. Genetic diversity indexes of the core collection constructedallele preferred sampling strategy were higher than those of other two strategies. 78 core collection resources of T. sinenseallele preferred sampling strategy accounted for 44.83% initial collection, and the retention rate of genetic diversity indexes(Na, Ne, H and I) were respectively 98.69%, 100.01%, 99.27%, 98.67%, which indicated that the genetic diversity and variation were well preserved. The result demonstrated that the allele preferred sampling strategy was the most appropriate method for core collection construction of T. sinense. References | Related Articles | Metrics

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Optimization of Ultrasound-assisted Extraction of Total Polyphenols from Moringa oleifera Leaves by Box-Behnken Response Surface Methodology FU Wen-qun,SUN Yan-yan,LI Xiao-hua,SHAO Nan-jin,HUANG Gui-hong,SU Xue-yan,ZHENG Yi-mei 2018, 47(02): 107-112.  DOI: 10.3969/j.issn.1009-7791.2018.02.002 Abstract ( )   PDF (3070KB) ( )   The optimum process of ultrasound-assisted extraction of total polyphenos from Moringa oleifera leaves by response surface methodology was studied in the research. The experiment method was designed according to Box-Behnken central composite design with 4 factors and 3 levels on the basis of single-factor experiment of ethanol concentration, material-to-liquid ration, extraction time and extraction times. The relationship between influencing factors and response values was subsequently established. The results showed that the optimum extraction conditions were as follows: the extracting solvent was 62% ethanol, material to liquid ration was 1:30 (W/V), extracting time was 30 min, and extracting three times. Under these conditions, the maximal yield of total polyphenols was upto 27.32 mg·g-1, which was well matched with the predicted content. References | Related Articles | Metrics

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Optimization of Extraction Technology of Total Flavonids from Rubus reflexus YAN Pei-pei,YE Qi,MO Zhi-long,WANG Xiao-hua,YE Wei,LEI Fu-gui,WEI Dao-zhi 2018, 47(02): 113-116.  DOI: 10.3969/j.issn.1009-7791.2018.02.003 Abstract ( )   PDF (3447KB) ( )   Extraction and purification technology of total flavonoids in Rubus reflexus were investigated by single factor study and orthogonal experiment. Results showed the optimal conditions were material to liquid ratio with 1:25, 60% ethanol concentration, 70 ℃, extracting 2.5 hours and extraction times with twice. Through the best extraction technology, the yield of R. reflexus total flavonoids could be up to 6.78%. References | Related Articles | Metrics

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Nutrient Components and Active Ingredients in Fagopyrum cymosum from Qinling-bashan Mountains ZHANG Yan,WANG Qing,CHEN Chen,LI Yang,LI Yan,ZHOU Jun-hui 2018, 47(02): 117-119.  DOI: 10.3969/j.issn.1009-7791.2018.02.004 Abstract ( )   PDF (2877KB) ( )   The nutrients and active ingredients of Fagopyrum cymosum from Qinling-bashan Mountains were analyzed. The results showed that F. cymosum from Qinling-bashan Mountains was rich in active ingredient, such as (-)-epicatechin, rutin, etc. The crude protein content in F. cymosum was higher (20.03%), which could be used as a breeding material. The total amino acid content was 15.78%, including essential amino acid (5.54%) and non-essential amino acid (10.24%). F. cymosum from Qinling-bashan Mountains should be a resource plant with abundant amino acid, and could be used as a multi-purpose plant variety with excellent wild forage resources and medicinal properties. References | Related Articles | Metrics

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Effect of Drought Stress on Non-Structural Carbohydrates in Cunninghamia lanceolata Clones LAI Xu-en,HU De-huo,SU Yan,ZHANG Yi-chi,ZHENG Hui-quan 2018, 47(02): 120-123.  DOI: 10.3969/j.issn.1009-7791.2018.02.005 Abstract ( )   PDF (5036KB) ( )   This study aimed to quantify needle starch, soluble sugar content, and non-structural carbohydrates (NSC) of six Cunninghamia lanceolata clones under PEG-6000 simulated drought environment. The results showed that drought stress led to a reduction of the needle starch and an increase of soluble sugar content. All the clones tended to lower their non-structural carbohydrate concentrations in response to drought stress (except for T-cF1). Significant positive correlations (P<0.05) were found between soluble sugar and relative water content and malondialdehyde. While significant negative correlations (P<0.05) were observed for starch and NSC with catalase respectively. References | Related Articles | Metrics

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Effect of Colchicine on Chromosome Structure Variation of Onion Root Tip Cells LIU Fei-ting,LI Qin,LI Yue,GUO Hai-bin,LI Nan 2018, 47(02): 124-127.  DOI: 10.3969/j.issn.1009-7791.2018.02.006 Abstract ( )   PDF (946KB) ( )   Treated with 0.05% and 0.1% colchicine solution by controlling time, effects of different colchicine concentrations and different treatment times on onion root tip distortion were researched. The results showed that colchicine had the ability to promote cell mitosis and stagnate cell chromosome in medium term. Different fixation times had effects on the mitosis of onion root tip cells. Colchicine had a distorted effect on onion root tip cells, and the ability to induce distortion could be influenced by colchicine solution concentration and treatment time. References | Related Articles | Metrics

Plant ecology, resources and taxonomy

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Anatomical Features of Vegetative Organs of Sesbania cannabina WU Xiao,ZHOU Jin-xin,WENG Le,CHEN Xin-lu,CHEN Mo-shun 2018, 47(02): 128-133.  DOI: 10.3969/j.issn.1009-7791.2018.02.007 Abstract ( )   PDF (14737KB) ( )   The anatomical structures of Sesbania cannabina vegetative organs were studied by paraffin and tissue segregation. The results showed that there were typical secondary structure characteristics of root of S. cannabina while there were casparian strip in the endoderm, a lot of nodules on the surface of root; rhizobia were distributed in the cortex of root, and its vascular tissue was connected to form a channel network. Cuticle appeared in epidermal cell walls of stem. There was specialized collenchyma in the cortex, which consisted of parenchyma cells. Vascular tissue had crystal phloem fiber. There were 3—4 stem nodules of different size, and distributing discontinuously. The outer layer of stem was cortex while the inner layer was bacteria tissue. The leaves were bifacial. The cuticle and epidermis of leaves were thick, and palisade tissues were well-developed, nearly half of leaf thickness. As for its sponge tissue, the intercellular space was large, and there were many small stomas. Xylems were rather rich in the root and stem of S. cannabina, with a number of large aperture vessels and rich wood fibers. Thickness rate of palisade and spongy and leaf palisade tissue tightness (CTR) were high. The anatomical features of S. cannabina showed its characteristics of nitrogen fixation and ecological adaptation of salt tolerance and drought resisting. References | Related Articles | Metrics

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Preliminary Study on Reproductive Features of Reintroduction Population of Cycas debaoensis WANG Yun-hua,GAN Jin-jia,CHEN Ting,GONG Yi-qing,LU Yan-zhao,LI Nan 2018, 47(02): 134-139.  DOI: 10.3969/j.issn.1009-7791.2018.02.008 Abstract ( )   PDF (5213KB) ( )   The reintroduction population of Cycas debaoensis in Huanglianshan nature reserve and its natural population in Fuping village of Guangxi were studied. Reproductive features, including number of flowering plant, male/female ratio, pollination, seed setting, seedling survival, were investigated. The results showed that: (1) In reintroduction and natural population, the number of flowering plants were 149 and 49, the male/female ratio were 3.96:1 and 4.44:1, the flowering plants account for 31.63% and 19.52%, and the natural seed setting rate were 60.53% and 86.53% respectively. (2) For unshed seeds, the seed germination rate were 53.13% and 42.51%, first-year seedling survival rate were 0 and 3.56%, respectively, while for the seeds spread by gravity and the external force (rainwater, wind and animal), the seed germination rate were 42.51% and 38.46% respectively, first-year seedling survival rate were 74.46% and 88.26% respectively. (3) As with Fuping natural population, the effective pollinator of C. debaoensis in Huanglianshan reintroduction population was Erotylidae beetles. In average, the amount of beetles in the reintroduction population was 23.5 per female cones while in the Fuping natural population was 32.1, the reintroduction population had less beetles than that in Fuping natural population. The following conclusions were drawn from the research: the seed setting rate of reintroduction population was lower than the natural population, which was probably due to the relatively small number of pollinators at reintroduction site. The seed dispersion by rodent and other external forces could significantly improve seedling survival rate and directly affect the planting and distribution of seedlings, and had important influence on replenishment and renewal of the population. On the whole, the reintroduction population of C. debaoensis in Huanglianshan had successfully completed the growth and reproduction process, and had the ability of self-renewal. References | Related Articles | Metrics

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Relationship Between Ravine Gradient and Plant Dominant Species in Yuanmou Dry and Hot Valley WU Shi-xiang,WANG Chen,HU Cui-hua,LIU Shou-jiang 2018, 47(02): 140-143.  DOI: 10.3969/j.issn.1009-7791.2018.02.009 Abstract ( )   PDF (446KB) ( )   With three typical plots (Shadi Village, Jinlei Village, and Julin Village) in the hot-dry valley of Yuanmou as sample areas, 48 plant ditch data about gullies and gully slope were collected, and important value methods were used, the dominant species of vegetation were discriminated and screened. The dominant species of shrubs and herbaceous were the main research objects. The relationship between dominant species of vegetation and gully slope was discussed. The results showed that: (1) The dominant species of shrubs were Dodonaea viscosa and Albizia kalkora, the important values were 0.486 and 0.342, respectively, accounting for 83% of the total shrubs. Herbaceous dominant species were Heteropogon contortus, Bothriochloa pertusa and Imperata cylindrica, the important values were 0.386, 0.140 and 0.196, respectively, accounting for 72% of the total herb. (2) Except for the significant correlation between the slope and coverage degree of I. cylindrica (P<0.05), there was no significant correlation between gully bottom slope and dominant species of shrubs or herbaceous dominant species (P>0.05). The results showed that in the dry-hot valley of Yuanmou area, the gully slope had little effect on growth of dominant species of vegetation. References | Related Articles | Metrics

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Dominant Populations and Their Age Structures of Forest Vegetation in Shimentai, Guangdong HUANG Lin-sheng,LI Yuan-qiu,DAI Ke-yuan,ZENG Yang-jin,MIAO Shen-yu,TAO Wen-qin 2018, 47(02): 144-148.  DOI: 10.3969/j.issn.1009-7791.2018.02.010 Abstract ( )   PDF (5625KB) ( )   Species diversity and age structures of the most five dominant species populations within an evergreen broadleaved forest in Shimentai National Natural Reserve of Guangdong province were analyzed. The results showed that there were 177 species (3593 individuals) belonging to 101 genera in 51 families and with diameter of breast height more than 1.0 cm. Among the 177 species, there were five arbor species with important values more than 10, the characteristic families were Theaceae, Fagaceae, and Lauraceae, etc. For the age structures of dominant species, the populations of Schima remotiserrata and Castanopsis hystrix were basically stable, while the populations of Rapanea neriifolia and Itea chinensis was at increasing stage, and the population of Schima superba were towards early declined stage. In general, the species composition of evergreen broadleaved forest vegetation of Shimentai was complex, and the structure was relative stable. References | Related Articles | Metrics

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Investigations on Medicinal Plant Resources in Xuwen County, Guangdong Province LIU En-tong,FANG Chu-nan,WU Xiao-dan,LIN Wei,LI Zhi-jie,WU Tian 2018, 47(02): 149-152.  DOI: 10.3969/j.issn.1009-7791.2018.02.011 Abstract ( )   PDF (440KB) ( )   To get a better knowledge on the present status of herbal resources in Xuwen county (Guangdong province) for providing reference to the utilisation of herbal resources, multiple investigations on medicinal plants through wildlife appraisal, sample collection and evaluation were carried out. The results indicated that there were 469 species of medicinal plants belonging to 100 families. Among them, there were 56 key species, and 28 speciality species for Chinese medicines in the Guangdong province. Given the limited distribution of wild herbal resources in Xuwen, emphasis should also be exerted upon protection of existing species. References | Related Articles | Metrics

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Vascular Plants Flora of Sanqu Stone Forest in Changshan County of Zhejiang Province YU Li-hong,LIU Guo-jun,ZHANG Jun-hui,YU Li-fang 2018, 47(02): 153-158.  DOI: 10.3969/j.issn.1009-7791.2018.02.012 Abstract ( )   PDF (448KB) ( )   Sanqu stone forest is a typical infant Karst landform. There were 472 species of vascular plants, belonging to 310 genera in 119 families. The vascular plants were not abundant and mainly distributed in small genera and small families. Herbaceous plants were dominated and accounted for 51.17%. The geographical elements of surveyed vascular plants consisted of 14 distribution patterns. The pan tropical, the north temperate and the east Asia distribution were dominated, accounting for 27.55%, 16.98%, 11.32% respectively. Compared with the adjacent area, tropical elements were highlighted. References | Related Articles | Metrics

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Analysis of Liverworts Diversity in Jinping Fenshuiling National Nature Reserve, Yunnan, China JIANG Ya,LI Ting-ting,YU Zhi-yong,LIANG Zong-li,MA Wen-zhang,PENG Tao 2018, 47(02): 159-170.  DOI: 10.3969/j.issn.1009-7791.2018.02.013 Abstract ( )   PDF (497KB) ( )   A liverwort (and hornwort) inventory for Jinping Fenshuiling National Nature Reserve was presented based on historical specimens and recent collections from 2015—2018. Totally, 203 species (including subspecies and varieties) from 60 genera in 34 families were recorded. Lejeuneaceae (57 species) and Plagiochilaceae (34 species) were the most species family, and smooth mat was the dominant life form among all liverworts. The flora of liverworts showed a clearly tropical characteristic (40.4%), which was considered as tropical elements. References | Related Articles | Metrics

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Chiastocaulon fimbriatum——A Newly Record of Liverworts from China JIANG Ya,LI Ting-ting,PENG Tao 2018, 47(02): 171-172.  DOI: 10.3969/j.issn.1009-7791.2018.02.014 Abstract ( )   PDF (9948KB) ( )   One species of liverworts, Chiastocaulon fimbriatum S. D. F. Patzak et al., was reported as a new record to China. Specimens were deposited in herbarium of Kunming Institute of Botany, Chinese Academy of Sciences (KUN). References | Related Articles | Metrics

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New Records of Ferns from Zhejiang Province WANG Zong-qi,XU Yuan-ke,LIN Jian,HOU Jian-hua,LI Qiao 2018, 47(02): 173-175.  DOI: 10.3969/j.issn.1009-7791.2018.02.015 Abstract ( )   PDF (8665KB) ( )   Four species of ferns were reported as new records from Zhejiang, China, Hymenopyhllum exsertum Wall. ex Hook., Vandenboschia cystoseiroides (Christ) Ching and Vandenboschia kalamocarpa (Hayata) Ebihara of Hymenophyllaceae, and Ctenitis dingnanensis Ching of Dryopyteridaceae. Specimens were deposited in herbarium of Jingning Forestry Bureau, Zhejiang Province. References | Related Articles | Metrics

Plant protection and cultivation

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Identification and Evaluation of Resistance of Amorphophallus spp. to Erwinia carotovora subsp. carotovora WU Xu,YANG Min,LIU Jia-ni,CHEN Ze-bin,WANG Ding-kang,ZHAO Jian-rong,ZHONG Yu,WU De-xi,YU Lei 2018, 47(02): 176-180.  DOI: 10.3969/j.issn.1009-7791.2018.02.016 Abstract ( )   PDF (9539KB) ( )   Soft rot disease of Erwinia carotovora subsp. carotovora in konjac is the most important diseases. The resistence to soft rot disease of Erwinia carotovora subsp. carotovora among 12 konjac (Amorphophallus spp.) cultivars were evaluated by different inoculation methods and fields disease survey. The results showed that A. konjac and A. albus were susceptible cultivars (S), A. bulbifer from Dehong city and Lincang city were highly resistant materials (HR), A. bulbifer from Myanmar was resistant materials (R), while A. muelleri was immune resistant (I). The results confirmed to fields disease survey. References | Related Articles | Metrics

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Comparative Study on Three-Dimensional Forest Cultivation of Dendrobium officinale LIANG Qiong-fang,CHEN Ting 2018, 47(02): 181-186.  DOI: 10.3969/j.issn.1009-7791.2018.02.017 Abstract ( )   PDF (6888KB) ( )   A comparative study about the survival rate, multiplication coefficients(MCF) and weight coefficient(WCF) of Dendrobium officinale under three-dimensional forest cultivations with planting pattern, the tree body and its parts as research object was carried out. The results showed that the survival rate of D. officinale under different planting patterns as follows: suspended tree segment (STE)(98.55%)> tree body(95.86%)> rock(92.67%)> seedbed (72.97%), and there was a significant difference between STE and other planting patterns. The survival rate of D. officinale in different trees was Artocarpus heterophyllus(96.43%)> Dimocarpus longan (96.22%)> Mangifera indica(95.37%) > Litchi chinensis(93.54%)> Pinus massoniana(32.93%), and there was a significant difference between previous three trees and others. The survival rate of the fork was the highest(86.89%) in different tree parts. The comparison of MCF and WCF under different planting modes found that the two coefficients of the epiphytic types were higher than seedbed, and significant difference existed between them. The coefficients of STE were the highest in all epiphytic types(MCF=3.50 and WCF=4.55 after three years), and there was significant difference from other epiphytic types. Therefore, the cultivation of D. officinale should mainly use STE pattern, and make the most of evergreen broad-leaved arbor and rock as the carrier to cultivate in stereo planting pattern. References | Related Articles | Metrics

Reviews

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Research and Application of SNP Molecular Markers in Ligneous Plants Genetics and Breeding ZHOU Lin,DUAN Yu,WEN Bo,MA Yuan-chun,ZHU Xu-jun,WANG Yu-hua,FANG Wan-ping 2018, 47(02): 187-193.  DOI: 10.3969/j.issn.1009-7791.2018.02.018 Abstract ( )   PDF (5086KB) ( )   Research progress of ligneous plants was restricted for their long life cycle, high heterozygosity, large genome and unclear genetic background. With the development of modern biotechnology, DNA molecular markers were applied in research of ligneous plants. Single nucleotide polymorphism (SNP), the third generation of molecular markers, has many advantages, such as high efficiency, rapidity, stability, reliability and so on, so it has been widely used. In order to provide a reference for better application of SNP technology in ligneous plant research, SNP markers characteristics, development methods, detection methods and its applications in genetic diversity and genetic relationship analysis, cultivar identification, genetic linkage map construction and molecular assisted breeding for ligneous plants were summarized. References | Related Articles | Metrics

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Reviews on Varieties and Genetic Breeding of Grapefruit DONG Mei-chao,LI Jin-xue,ZHOU Dong-guo,YANG Fan,GUO Li-na,LIU Hong-ming,GAO Jun-yan 2018, 47(02): 194-198.  DOI: 10.3969/j.issn.1009-7791.2018.02.019 Abstract ( )   PDF (435KB) ( )   The researches on grapefruit origin, distributing, variety evolution, introduction and cultivation status, variety selection and molecular genetics were reviewed in this paper. Besides, the survey of resources research of grapefruit and its utilizable value were also summarized. References | Related Articles | Metrics