Subtropical Plant Science

The Effects of Acid and Aluminum on Ginkgo Growth under the Soilless Culture

LI Zhong-fang, YU Qiu-liang, YANG Zhi-xian, ZHU Xiu-feng, LU Fa-jia

2012, 41(02): 1-5. DOI: 10.3969/j.issn.1009-7791.2012.02.001

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The effects of Ginkgo growth was studied under the coupling treatment of acid and aluminum (Al) by applying U₂₀₇ even experimental design and soilless culture. The results showed that Ginkgo could grow normally at the concentration of Al³⁺of 0.4 mmol/L and below in the range of pH 4.5～5.5, while it could be damaged under the high concentration of Al³⁺ within 0.8~1.2 mmol/L. On other hand, different concentrations of Al³⁺caused no effect on Ginkgo growth when the pH was higher than 5.0. In a word, Ginkgo growth would be affected especially root system by the coupling treatment of high concentration of Al³⁺ and low pH with the roots got dark and the new roots reduced, even died or rotted, and the plantlet would stop to grow.

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Effects of Phytic Acid on Physiological Characteristics of Germination of Hybrid Rice Seeds

DING Jun-hui,PENG Ying-long,OU Ou,

2012, 41(02): 6-8. DOI: 10.3969/j.issn.1009-7791.2012.02.002

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Hybrid rice seeds V20B were used as experimental material to study the effect of exogenous phytic acid on physiological characteristics of germination of hybrid rice seed. The results showed that seed germination was significantly inhibited by 10 mmol/L exogenous phytic acid. Physiological and biochemical analysis indicated that α-amylases activity, soluble sugar content, and phytic acid were affected in different degrees by exogenous phytic acid during seed germination．

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Prokaryotic Expression and Optimization of Glucosyltransferases PlUGT2 from Pueraria lobata (Willd.) Ohwi

LI Jian-mei,LV Min,HU Xiao-xiang,LI Ling

2012, 41(02): 9-12. DOI: 10.3969/j.issn.1009-7791.2012.02.003

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Based on the single factor of experiment, the effects of temperature and IPTG concentration on BL21(DE3)/pProEXHTa-PIUGT2 protein production were studied. Fermentation conditions of BL21(DE3)/pProEXHTa-PIUGT2 cultured in 150 ml LB medium were optimized. The expression conditions of PlUGT2 protein was high when culture condition to be 0.75% of IPTG concentration and 20℃.

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Quality Research of Compound Prescription Cortex Phellodendri Chinensis Fluid

CHENG Li-hui,LU Li-xia

2012, 41(02): 13-18. DOI: 10.3969/j.issn.1009-7791.2012.02.004

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To establish the research method for compound prescription cortex phellodendri chinensis fluid, the berberine hydrochloride and chlorogenic acid in compound prescription cortex phellodendri chinensis fluid were tested by the method of TLC and HPLC. The results showed that spots obtained from the sample solutions had the same color as their authentic sample solutions, the blank solutions had no interference in TLC of compound prescription cortex phellodendri chinensis fluid. The content of berberine hydrochloride in the range of 0.09 to 0.45 μg had good linear relationship, the average recovery was 103.4%; the content of chlorogenic acid in the range of 0.9 to 4.5 μg had good linear relationship, the average recovery was 100.3%. In conclusion, this method can be used in the quality control of compound cortex phellodendri chinensis fluid.

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Effects of Different Cytokinins on Induction of Potato Sterile Plantlet

YU Ru-gang,DU Xue-ling,SONG Yun-xian

2012, 41(02): 19-22. DOI: 10.3969/j.issn.1009-7791.2012.02.005

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Shoot tips of ‘Highland No.7’ potato are selected as explants and 0.1% Mercuric Chloride and 75% alcohol are employed to disinfect; then through tissue culture, virus-free potato stems are obtained. In the process of enrichment culture, the paper tries to analyze the effect that 6-BA and KT with different consistency have on proliferation-inducing result of potato stems. The result indicates that it is the most effective to use 0.1% Mercuric Chloride to disinfect the shoot tips for 8 minutes, by which the disinfection rate can be up to 96.67%. The better promoting effect of potato stem proliferation made by cytokinin can be achieved when 6-BA > KT; adventitious bud induction and growth potential are the best when the consistency of 6-BA reaches 1.5 mg/L.

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The Study on Callus Inducement and Differentiation of Draba nemorosa

ZHU Ting-ting,YAN Wei-yu,LI Xiang-fen,NING Shu-xiang,JIANG Chang-yang

2012, 41(02): 23-26. DOI: 10.3969/j.issn.1009-7791.2012.02.006

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In order to preserve the wild resources and meet the needs of cultivation, with the method of tissue culture, tender stems of Draba nemorosa were used as material to study on callus inducement and differentiation, rooting of adventitious buds, rooting multiplication of tube seedlings, replantation and field planting. The results showed that MS+6-BA 0.4 mg/L+2,4-D 2.5 mg/L was the ideal medium for inducing callus and callus multiplication; MS+6-BA 0.6 mg/L+NAA 0.1 mg/L was suitable for callus differentiation and multiplication of adventitious buds; the best medium for rooting of adventitious buds and rooting multiplication was 1/3MS+0.6 mg/L; the transplanting survival rate of tube seedlings was 86.8% and stable planting survival rate was 96.4%; the seedlings planted in field were maintained all traits of the wild Draba nemorosa.

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Rapid Propagation and In vitro Conservation of Dendrocalamus yunnanicus

LI Chun-fang,CHEN Zhi-ying,YANG Jun-bo,XIONG Zhi

2012, 41(02): 27-31. DOI: 10.3969/j.issn.1009-7791.2012.02.007

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The caryopsis of Dendrocalamus yunnanicus, which had been stored for 150 days at -20℃ and RH 15%, were used as the experimental materials, and aseptic clones were induced from them then used for propagation and in vitro conservation. The results showed that the best media for tufted-bud proliferation were MS + 6-BA 2 mg/L + NAA 0.2 mg/L, both sucrose and glucose contributed to the proliferation culture, and the best concentration was 3%. The most effective medium for rooting was MS + IBA 1 mg/L + NAA 1 mg/L, a single bud rooting was better; the best medium for buds in vitro conservation was MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L. The subculture cycle of shoots was 60 days at 25±3 ℃, which could change to 160 days and 240 days at low temperature preservation 20±3 ℃ and 12 ℃, respectively. Albino seedlings or variant of leaf colors were found during tissue culture.

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Study on the Growth Characteristic of Young Trees of Bretschneidera sinensis

GUO Xiang-quan,ZHOU Li-hua,XION Zi-hua,SUN Xiao-dong,GUO Xiang-dui,WU Zai-zhang,LIN Jian-li,ZHENG Jing-chi,PEN Xiao-yong

2012, 41(02): 32-36. DOI: 10.3969/j.issn.1009-7791.2012.02.008

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The growth characteristics of height, root collar diameter and lateral branch of 5-year-old Bretschneidera sinensis trees were investigated. The results indicated that the averages, max, minimum of height and root collar diameter were respectively 2.0 m, 3.4 m, 0.5 m and 3.2 cm, 9.5 cm, 0.5 cm.The growth distributions of height and root collar diameter kept to the normal distribution. The growth of height and root collar diameter were evenly differentiated in treelet group. The current growth of height and root collar diameter of 1 to 5-year-old trees showed that the growth of height was lower in 3-year ago and higher at the 4 and 5-year. The growth of root collar diameter was bigger in the first year. The young trees which height were less than 2.0 m were not found lateral branch growth and their crown diameter was narrow and height growth was slowly.

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The Influence of Planted Slope and Soil Preparation Time on the Growth of Catalpa bungei Seedlings

LIU Tong-gao

2012, 41(02): 37-39. DOI: 10.3969/j.issn.1009-7791.2012.02.009

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Adopting a random area set survey method, the relationship of annual Catalpa bungei seedlings growth rate and different slope, different soil preparation time in Fujian northeast area was studied. The results showed that annual Catalpa bungei seedlings were the survival rate of 98.2%, the seedling height of 0.97 m and the ground diameter of 1.65 cm. Catalpa bungei seedlings were suitable for growth of south slope, and the seedlings growth would be inhibited at north slope. The soil preparation time would be chosen in late autumn and early winter.

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Effect of Different Thinning Intensity on the Growth of Cunninghamia lanceolata

LIN Hua-zhang

2012, 41(02): 40-42. DOI: 10.3969/j.issn.1009-7791.2012.02.010

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In this paper, the influences of different thinning intensity on the growth of Cunninghamia lanceolata plantations were investigated in Taoyuan National Forest Farm, Fujian province. The results indicated that the net growth of plant height, diameter at breast height (DBH) and individual volume was increased by 5.7%~37.1%, 12.5%~112.5% and 15.0%~129.8% respectively in C. lanceolata plantation treated by different thinning intensity of 30%, 45%, 60% compared with the control plantation after thinning of six years. Obviously, the plant height, diameter at breast height, individual volume of the plantation with thinning intensity of 60% were increased most significantly among them. However, with the increase of thinning intensity, the stand total volumes had a little decrease. On the basis of these, some suggestions were put forward to big, medium, small diameter timber of C. lanceolata, including thinning number and time, and the cultivation period.

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Studies on Hole Tray Cutting Propagation of Nerium indicum ‘variegata’

ZHANG Ling,WANG Tian,WANG Hua,YE Hui

2012, 41(02): 43-47. DOI: 10.3969/j.issn.1009-7791.2012.02.011

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The orthogonal design L₉(3⁴) was conducted to study the effects of cuttings types (A), three different growth regulators(B) and their concentrations(C) on rooting of Nerium indicum ‘variegata’ cuttings and their physiological characteristics. The results showed that the main influencing factors were cuttings types and growth regulator species, and the best combination was A₁B₁C₁ that using the lesser degree of lignification of upper branches as cuttings treated by naphthalene acetic acid (NAA) with the concentration of 200 mg/L.

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SHI Jian-yu

2012, 41(02): 48-50. DOI: 10.3969/j.issn.1009-7791.2012.02.012

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The effects on rooting of Osmanthus fragrans var. semperfloren cutting by different cuttage matrix, branch maturity and leaves remaining were researched in this paper. The result showed that the effects on rooting of O. fragrans var. semperfloren cutting by different cuttage matrix and branch maturity had significant differences. However, the effect on rooting of O. fragrans var. semperfloren cutting with one leaf and with two leaves was unobvious. Therefore, it was the best conditions for rooting of O. fragrans var. semperfloren cutting that the branch was half-lignification, the cuttage matrix was 2/3 peat soil and 1/3 pearlstone, and the leaves remaining was one leaf or two leaves.

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The Investigation on Tree Species of Magnoliaceae Resources and Its Flowering and Fruiting Period in Nanyue Mountain

XIA Lin-lin,XIA Jiang-lin,LIU Yu,YANG Zhi-long,WANG Xu-fu

2012, 41(02): 51-54. DOI: 10.3969/j.issn.1009-7791.2012.02.013

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The investigation on the flowering and fruiting period and the species statistics of tree species of Magnoliaceae in Nanyue Mountain were carried out. The results showed that there were 7 genera and 60 species in Nanyue Mountain including the grade of infra-species. Among them, there were 42 species belonging to 6 genera blossomed and 33 species belonging to 6 genera were observed to fruit. Some species flowered twice a year and florescence over long periods, and many flowers of them smell sweet. These investigations provided basic materials to the landscape application of tree species of Magnoliaceae.

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Additional Materials on Wild Resources of Fruit Plants in Jiufu Mountain, Fujian Province

WEI Kai-ju,CHEN Qiao-ming

2012, 41(02): 55-58. DOI: 10.3969/j.issn.1009-7791.2012.02.014

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On the basis of the former references on wild fruit plants in Jiufu Mountain, Fujian Province, some additional species of wild fruit plants in the mountain were reported in this paper. The investigation showed that there were 8 varieties and 55 species belonging to 46 genera and 30 families wild fruit plants in this area, which hadn’t been mentioned in former studies. At the same time, their family name, scientific name, characteristic, the fruiting period, the type of fruit, the usage of fruit, the habitat and the altitude of distribution were listed. Some suggestions about the development and utilization of these wild fruit plant resources were put forward in this paper.

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New Distribution Record of Angiosperm in Fujian Province (Ⅱ)

YANG Cheng-zi,LIU Xiao-fen,HUANG Ze-hao,FAN Shi-ming,SHEN Xian-juan,WU Jin-zhong

2012, 41(02): 59-61. DOI: 10.3969/j.issn.1009-7791.2012.02.015

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According to the investigation on the vegetation in Fujian Province, 5 newly recorded species, Euonymus alatus, Mazus procumbens, Rungia densiflora, Siraitia siamensis, Eulophia graminea of Angiosperm were found. The voucher specimens were deposited in the Herbarium of FJTCM.

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The Introduction of Five Varieties of Spathiphyllum

CHEN Han-xin,WAN Xue-feng,KANG Yue-hui,ZHANG Liang-shui,WU Wei-hua

2012, 41(02): 62-64. DOI: 10.3969/j.issn.1009-7791.2012.02.016

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This paper deals with the description of the morphological characters and growth habits of five varieties belonging to Spathiphyllum, and the techniques of cultivation and management were mentioned in here.

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