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10 September 2008, Volume 37 Issue 03 Previous Issue    Next Issue

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Dynamic Changes of Endogenous Hormones and Polyamine in Phalaenopsis Leaves during Floral Bud Formation ZENG Xin-ping, LIU Zhi-cheng, SU Ming-hua, CHEN Chun 2008, 37(03): 1-5.  Abstract ( )   PDF (930KB) ( )   Phalaenopsis mature seedlings were treated under 17℃/27℃(night/day) to study the dynamics of endogenous hormones and polyamine in leaves during floral bud formation. The results showed that the key time for flower formation was 15d, the weight of buds and contents of endogenous hormones and polyamine varied significantly on the 15th day after treated. Low level of GA3 and ABA, high contents of ZR and Spd, high ratio of ZR to GA, ZR to ABA, Spd to Put were better for flower bud formation. Relative high content of endogenous ZR was the key factor of floral bud formation. The correlation between the content of endogenous polyamine and hormones was also observed. Related Articles | Metrics

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Water Capacities of Soil in Different Vegetation Types in Dabie Mountains, Anhui Province ZHUANG Jia-yao, ZHANG Yong, WU Zhong-neng, HU Zhi-dong 2008, 37(03): 6-9.  Abstract ( )   PDF (866KB) ( )   The water conservation capacity of soil was studied in cultivated land, Chinese fir forest (middle aged and matured), bamboo forest and pine forest (middle aged and matured) in Shangshe catchment, Dabie Mountains of Anhui Province. The results showed that the values of water conservation capacity of soil in the forests varied from 48 129 to 69 488t/km2. They were 1 to 2 times higher than that in the cultivated land at slope. In order to hold one billion m3 water by soil dam, the area needed for forests varied from 1 439 to 2 078km2, while the area needed for the cultivated land at slope was 3 747km2. Related Articles | Metrics

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Studies on Genetic Diversity of 48 Tobacco Varieties by RAPD Analysis YANG You-cai, YU Shuo-hui, YIN Han-qi 2008, 37(03): 10-14.  Abstract ( )   PDF (1459KB) ( )   RAPD molecular-marker technique was used to detect the genetic diversity among 48 tobacco germplasms. The genomic DNA of 48 tobacco germplasms was tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 arbitrary 10-mer primers. The results showed that the 184 RAPD bands were obtained altogether with 86 bands of polymorphic ones. The percentage of polymorphic bands was 46.7%.The variation range of genetic distances was 0 to 7.81. These varieties were divided into N. tobacum group and N. rustica group by UPGMA cluster analysis based on RAPD molecular-markers data. N. tobacum group could be divided into 4 sub groups. Related Articles | Metrics

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Photosynthetic Characteristics of Banana (Musa AAA Group Cavendish Williams) Leaf in Field LIU Wan-gou, WANG Yue-xia 2008, 37(03): 15-18.  Abstract ( )   PDF (962KB) ( )   A field study on diurnal variation of net photosynthetic rate (Pn), light response curve and CO2 response curve in leaves of Musa AAA Group Cavendish Williams was conducted using Li-6400 portable photosynthetic gas analysis system. Some photosynthetic parameters were estimated using non-rectangular hyperbola model combined with nonlinear regression of SPSS software. The results indicated that diurnal variation in Pn presented a single peak curve, with no apparent midday depression, and the peak value of Pn appeared at about 10:20 am. The light saturation point (LSP), light compensation point (LCP), apparent quantum efficiency (AQY), CO2 saturation point(CSP), CO2 compensation point(CCP) and carboxylation efficiency (CE) were 778.19 μmol/m2·s, 61.09μmol/m2·s, 0.0414, 608.97μmol/mol, 72.13μmol/mol and 0.094, respectively. The main environmental factors influencing on Pn were also discussed, and it showed that under sufficient soil water supply, photosynthetic active radiation rather than stomatal conductance (Gs) limited the Pn. Related Articles | Metrics

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Relationship between Physiological Drop and Some Physiological and Biochemical Indexes of Citrus grandis ‘Tomentosa’ ZENG Qing-qian, CAI Yue-wen, YAN Zhen, LI Xiang-ming 2008, 37(03): 19-21.  Abstract ( )   PDF (856KB) ( )   Physiological and biochemical indexes in normal fruits and fallen fruits were determined to reveal the physiological mechanism on Physiological drop of Citrus grandis ‘Tomentosa’. The results showed that contents of N, P, K, Ca and Mg in normal fruits were higher than those in fallen fruits; Contents of total sugar in fallen fruits were declined constantly and contents of total sugar in normal fruits raised and then declined; Contents of promoting endogeny hormone including GA3、IAA、ZT in normal fruit were markedly higher than those in fallen fruits, likewise, ABA/(GA3+IAA+ZT) of fallen fruits was significantly higher than that of normal fruit; The activities of three hydrolase in fallen fruits were significantly higher than those in normal fruits. Fruit drop of Citrus grandis ‘Tomentosa’ was correlated with the contents of mineral nutrition, total sugar and endogeny hormone and hydrolase activity in fruits. Related Articles | Metrics

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Comparison of Storage Effects Between on-tree and at Low Temperature of Citrus sinensis ‘Newhall’ Fruit XU Yong-jie, XIA Ren-xue, LUO Zhi-jian, WANG Yong, DONG Tao 2008, 37(03): 22-25.  Abstract ( )   PDF (887KB) ( )   Newhall navel orange(Citrus sinensis ‘Newhall’) fruits stored on-tree and at low temperature were used to study the storage effects of different storage methods. The flesh firmness, total difference of colors, active oxygen species(AOS), content of prolin(Pro) in one gram FW, relative of electric conductivity, the content of malondialdehyde (MAD), and the activity of lipoxygenase(LOX), superoxide dismutase(SOD), catalase(CAT) were determined in the peel and pulp of fruit. We observed that during the storage the flesh firmness dropped, the total difference of colors and the activity of LOX raised, the activity of SOD, CAT descended, AOS, MDA, Pro accumulated, resulting in the injury of membrane of fruit cell, the flowage of substance in cell and rising of the relative of electric conductivity. It meant that the injury degree of fruit cells was increasing during storage of the two methods. And the injury did not result from just one factor, but the interaction of these factors. The comparison of the two methods showed that, there were no significant difference in the first 47 storage days, but during the storage of 47d to 75d, there was obvious superiority of the fruit from storage on-tree, no matter what the appearance (flesh firmness and total difference of colors) and the integrity of cell membrane. So the storage on-tree had more superiority than storage at low temperature of both economizing of cost and improvement of the fruit. Related Articles | Metrics

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A Study on Photosynthetic physiology of Poplar ‘Zhongxin 4’ in Summer YANG Wei-ping, WU Rui-yun, DAI Yue 2008, 37(03): 26-30.  Abstract ( )   PDF (915KB) ( )   The photosynthetic physiology of poplar ‘Zhongxin 4’ (Populus deltoides CL. ‘Zhongxin 4’) was studied in this paper. The result showed that the net photosynthetic rate (Pn) decreased in high temperature stress (above 32℃), and stomatal limitation was the most important factor at early stage. With the elongation of time in high temperature and reduction of relative humidity(RH), stomatal and non-stomatal limitation acted simultaneously. The heat tolerance of poplar ‘Zhongxin 4’ varied with RH. When RH decreased, the temperature stress increased, the solar energy utilization efficiency decreased. Under humid condition, Pn was positively correlated with photosynthetic active radiation (PAR). Under arid condition, Pn was positively correlated with RH. Related Articles | Metrics

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Preliminary Report on Seed Culture in vitro of Four Wild Orchidaceous Plants ZENG Bi-yu, ZHU Geng-fa, LIU Hai-tao, WANG Bi-qing 2008, 37(03): 31-34.  Abstract ( )   PDF (1845KB) ( )   The traits of fruits, seeds, embryos and seeds culture of four wild orchidaceous plants were studied. These plants included Calanthe discolor; Liparis japonica; Pholidota chinensis; Dendrobium densiflorum. The results showed that the colours of the seeds were white, light green or yellowish brown. The seeds were spindle or ellipsoid. The seeds of L. japonica, P. chinensis, D. densiflorum had seed pores and their embryos were dense and visible. The seeds germinated early and the germination rate was high. In which, the seeds of P. chinensis and D. densiflorum germinated earliest, and it only needed 36 days. The germination rate reached 100%. But the seeds of C. discolor did not have seed pores and the embryos could not see clearly. The seeds germinated late and the germination rate was low. The seeds of four orchidaceous plants were germinated by protocorms. A group of aseptic seedlings had been achieved through subculture. The germination status was discussed in combination with the characteristics of fruits, seeds and embryos. Related Articles | Metrics

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Flavones Extraction from Longan's Aril and Identification by Ethanol Method HUANG Ai-ping, ZHENG Shao-quan 2008, 37(03): 35-37.  Abstract ( )   PDF (879KB) ( )   Based on the qualitative test and quantitative test, the ‘Chuliang’ Longan's aril was extracted by the ethanol extraction method. The studying identified that the Longan' aril contained the flavones. The maximum absorption of lutin standard was at 510nm and the standard curve was A=9.33C+0.013 (R=0.9986). The total of flavones were 15.48±0.27 (mg/100g·FW). RSD was within 1.75%. The recovery was 103.3%±3.65% and RSD was within 3.53%. The result will help to proceed with extracting flavones of Longan's aril and activity assays, and its theoretical base is established. Related Articles | Metrics

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Polyphenol Contents and Antioxidant Activity of Various Solvent Extracts from Scurrula parasitica Leaves LAI Jing-jing, CHEN Bing-hua, LIAN Jun-rui, CHEN Yu-fang, HOU Wang, HUANG Zhang-guan 2008, 37(03): 38-42.  Abstract ( )   PDF (1124KB) ( )   The antioxidant activities of the different polarity solvents extracts from Scurrula parasitica leaves were evaluated by the DPPH assay, TEAC assay, FRAP assay. In addition, the total phenolic and flavonoid contents of them were also determined. The resulted showed that the properties of the extracting solvents significantly affected the yield, the total phenolics, flavonoids and antioxidant activities of S. parasitica extracts. The three different solvent extracts showed varying degrees of efficacy in three assays in a dose-dependent manner. For three different solvent extracts, the antioxidant activities were in this order:80% acetone extract >80%methanol extract >water extract. 80% acetone extracts with the highest amount of total phenolics (276.83 mg tannic acid equivalents/g), exhibited the highest antioxidant activity with value of EC50 0.247 by the DPPH assay, the value of FRAP 115.81 by the FRAP assay, the value of TEAC 2.04 at 1.0mg/mL by the TEAC assay. Related Articles | Metrics

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A Study on the Tissue Culture of Phalaenopsis spp. LIU Liang, YI Zi-li, JIANG Jian-xiong, CHEN Zhi-yong, HUANG Li-fang 2008, 37(03): 43-45.  Abstract ( )   PDF (841KB) ( )   The experiment studied in the tissue culture of Phalaenopsis spp.. The results showed that in 1/2MS medium with 6-BA3.0mg/L+NAA0.2mg/L or with 6-BA 6.0mg/L, the induction rate of clustered shoots reached 100%. During the proliferating period of clustered shoots, the 1/2MS medium with 6-BA5.0mg/L+NAA0.5mg/L showed the best effects; in 1/2MS medium with 6-BA 15.0mg/L+NAA 1.0mg/L, the induction rate of protocorm-like body reached 20%, and in 1/2MS medium with 6-BA3.0mg/L+NAA0.5mg/L, the coefficient of reproduction is up to 7.4. It was easy for protocorm-like body differentiation to buds in 1/2MS+6-BA1.0mg/L+NAA0.5mg/L. In rooting stage, the 1/2MS medium with IBA0.3 mg/L was efficient. Transplanted them on water moor, and its survive rate reached 100% after two months. In addition, it was more conducive to the growth by water culture in the late cultivation. Related Articles | Metrics

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Rapid Propagation System of 4 Genotype of Brassica napus HONG Sen-rong, YIN Ming-hua, WANG Ai-ping, XU Wei-hong, SHI Wen-juan, WANG Yu 2008, 37(03): 46-48.  Abstract ( )   PDF (1508KB) ( )   Single factor test and plant tissue culture methods were applied to establish the aseptic system of Brassica napus and study the proliferation and rooting of stem with a bud. The best disinfection way of Brassica napus seed was using 70% ethyl alcohol for 30 seconds then using 0.1% HgCl2 for 8~10 minutes. MS+6-BA 2mg/L+NAA 1~2mg/L gave the best result for proliferation of Brassica napus stem with a bud and MS+NAA 2~5mg/L was best for rooting. Related Articles | Metrics

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Effects of Uniconazole on Potato Plantlets in vitro ZHOU Zu-fu, LI Zhao-an, YANG Mei-chun, ZHOU Qi-wei 2008, 37(03): 49-50.  Abstract ( )   PDF (803KB) ( )   0.001~0.005mg/L of Uniconazole retarded the growth of potato plantlets in vitro effectively, made the plant height lower, the stem diameter wider, the plant weight heavier and the green color of leaves thicker, and increased the adventitious root number and diameter. 0.003mg/L of Uniconazole yielded the best result, while 0.005mg/L of Uniconazole caused serious dwarfing of the plantlets. Related Articles | Metrics

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Technique for Micro Propagation of ‘Aweilan’ Rose CHEN Yong-kuai, ZOU Hui, JU Yu-dong 2008, 37(03): 51-54.  Abstract ( )   PDF (884KB) ( )   ‘Aweilan’ rose was cultured on MS medium supplemented with different concentrations of hormones (6-BA and NAA). The induction and growth of plantlets were observed and the effects of the concentration of hormones and active carbon on the differentiation of ‘Aweilan’ rose were studied. The results indicated that culture medium MS with 6-BA 2.0mg/L and NAA 0.3mg/L was good for induction of accessory bud, MS medium with 6-BA 2.0mg/L and NAA 0.1mg/L was favorable for bud-differentiation,and medium 1/2 MS with NAA 0.5 mg/L gave the best result for rooting. Related Articles | Metrics

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The Alien Invasive Plants in Guilin City, Guangxi Zhuangzu Autonomous Region CHEN Qiu-xia, WEI Chun-qiang, TANG Sai-chun, PU Gao-zhong, PAN Yu-mei 2008, 37(03): 55-58.  Abstract ( )   PDF (884KB) ( )   69 species of invasive plants belonging to 53 genera and 20 families are identified and recorded in Guilin based on investigation and referring to the related literatures. Among them, Compositae is the most consisting of 21 species, and Gramineae and Amaranthaceae are the second comprising respectively 9 species. Some of them are quarantined weed in China, such as Xanthium italicum and Sorghum halepense. Their origins, habitats, invasive approaches as well as damage degree are also investigated and analyzed. Some suggestions for preventing and controlling the alien plant invasion in Guilin are also discussed. Related Articles | Metrics

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Characteristics of Machilus versicolora Community Life-form in Mangdangshan, Fujian Province HUANG Zi-liang 2008, 37(03): 59-62.  Abstract ( )   PDF (894KB) ( )   The species of Machilus versicolora community are rich in Mangdangshan Natural Reserve of Fujian Province. There exists 133 species of vascular plants belonging to 102 genera in 69 families. The phanerophytes are absolutely dominated in the community, which possess 103 species and occupy 77.4 percent in all species. Among them, therophytes of the highest peak, a total of 37 species, which is 27.8 percent in all species. There are 25 lianas, which occupy 18.8 percent in all species. The characteristic of evergreen, microphyll, single, herbaceous and non-entire leaf dominated the community. The flora was characterized with obvious middle subtropical evergreen broad-leaved forest. Related Articles | Metrics

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Study on the Tree Species Evaluation of Urban Forest in Xiamen YE Zhi-yong 2008, 37(03): 63-66.  Abstract ( )   PDF (847KB) ( )   The masson pine ecological forest was rebuilt on the island of Xiamen. 44 kinds of broadleaf trees interplanting in the forest were investigated to evaluate their adaptability, growth characteristics and resistance. The results indicated that Schima superba, Acacia mangium and other 13 species were of high adaptability, Eucalyptus grandis×E. urophylla, Acacia mangium and so on 7 species were of good growth characteristics. Combining the visual effects, aesthetics and other aspects of a comprehensive evaluation, Liquidambar formosana, Schima superba, Ficus lacor, Acacia mangium, Cinnamomum japonicum, Ficus microcarpa var. pusillifolia, Elaeocarpus sylvestris, Castanopsis hystrix, Cinnamomum camphora, Eucalyptus grandis×E. urophylla, Myrica rubra were selected as backbone species for forest form rebuilding. Related Articles | Metrics

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In vitro Propagation of Adiantum reniforme var. sinense LI Hong-lin, GAO Li, YANG Bo 2008, 37(03): 67-67.  Abstract ( )   PDF (767KB) ( )   Related Articles | Metrics

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Tissue Culture of Sesbania cannabina GUO Tao, YANG Xiao-bo, LI Dong-hai, LONG Wen-xing, CAO Shi-wei, LI Shi-wei 2008, 37(03): 68-68.  Abstract ( )   PDF (768KB) ( )   Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Lilium rosthornii WANG Yuan-zhong, SHA Ben-cai 2008, 37(03): 69-70.  Abstract ( )   PDF (954KB) ( )   Related Articles | Metrics

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Reactive Oxygen Species and Phytohormone Signaling Transduction Pathways LIU Can, LI Ling 2008, 37(03): 71-75.  Abstract ( )   PDF (922KB) ( )   Plants can accept environment stimulation, specifically modulate gene expression, induce physiological responds, and adapt environment by complicated signal transduction. Reactive oxygen species (ROS) are versatile molecules mediating a variety of cellular responses in plant cells as an important signal molecule. ROS have been implicated as second messengers in several plant hormone responses. In this article, we review the role of ROS in phytohormone signal transduction. Related Articles | Metrics

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A Review of Antimicrobial Substances of Marine Algae LIN Xiong-ping, ZHENG Yi, CHEN Xiao-qing 2008, 37(03): 76-80.  Abstract ( )   PDF (1059KB) ( )   The chemical compounds of antimicrobial substances from marine algae in recent years were reviewed and the research prospect of antimicrobial activities from marine algae was given. Related Articles | Metrics

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Hydraulic Signal and Root-to-shoot Communication Signaling of Water Stress in Plants LI Xing-juan 2008, 37(03): 81-84.  Abstract ( )   PDF (975KB) ( )   The shoot response to limited soil water supply is not affected by the ABA generated in the root, but by the hydraulic signal generated in the shoot. The hydraulic signal communicates root-to-shoot signaling of water. Water stress in the soil results in hydraulic response in the shoot, then ABA signal is generated, and stomata close. Attenuating hydraulic signal can prevent the long-distance communication of water status in plants, and stomata can't close. Related Articles | Metrics