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10 November 2007, Volume 36 Issue 04 Previous Issue    Next Issue

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Changes of PAL, PPO, POD Activities in Different Papaya Cultivars Infected with Papaya Ringspot Virus LI Hui-hua, Xie Zhi-nan, LAI Rui-yun, SU Ming-hua 2007, 36(04): 1-4.  Abstract ( )   PDF (184KB) ( )   Five papaya varieties with different disease resistance were infected with Papaya ringspot virus, the activities of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) in papaya leaves were determined. The results indicated that the differences of the activities of PAL、PPO、POD among five papaya varieties before infected were small, and the descending order of enzyme activities was ‘Malai-10’, ‘Mihong-3’, ‘Malai-6’, ‘Malai-2’, ‘Tainong-2’. After infected, the activities of PAL、PPO、POD of five papaya varieties were higher significantly than those of their respective control, the change of enzyme activities in ‘Malai-10’ was most significant and that of ‘Tainong-2’ was slowest. Related Articles | Metrics

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Cloning and Sequencing of 18S-26S rRNA and ITS on Canarium album in Fujian Province SONG Ya-na, CHEN Rui-qing, ZHOU Li-juan, ZHENG Wei-wen 2007, 36(04): 5-9.  Abstract ( )   PDF (357KB) ( )   Cloning and sequencing were applied to analyze 18S-26S rRNA and ITS on 14 varieties of Canarium album.The results showed that the 14 varieties of Canarium album could be partitioned to 4 groups.Taking No.1 variety as control sample, the sequence of total 634 nucleotides in ITS1, ITS2 and 5.8S was same on No.3, 4, 6, 7, 8, 9, 11 variety, and only one nucleotide differed from the control in ITS2, and No.2 variety had two nucleotides differed from the control in ITS2, and the 9 varieties above belonged to the first group.The sequences of No.5 and No.14 were same, and had one nucleotide difference in ITS1 and ITS2 respectively compared to the control, No.5 and 14 varieties belonged to the second group.The sequences of No.10 and No.12 were same, and had two different nucleotides in ITS1 compared to the control, and No.10 and No.12 varieties belonged to the third group.The last group was No.13 variety, which had one and twenty-four different nucleotides in ITS1 and ITS2 respectively compared to the control.The homology tree established by DNAMAN showed the variation among the 14 varieties of Canarium album.And the sequence of Canarium album deposited in GenBank under number DQ517524. Related Articles | Metrics

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Cryopreservation of Pueraria lobata Shoot-tip in vitro by Vitrification and Plant Regeneration HONG Sen-rong, YIN Ming-hua, SHAO Xing-hua 2007, 36(04): 10-12,15.  Abstract ( )   PDF (373KB) ( )   The cryopreservation technique of Pueraria lobata shoot-tip was studied in this paper.The results showed that the better procedure of cryopreservation by vitrification was as follows.The aseptic plantlets of H# Pueraria lobata subcultured in vitro for 30 days were treated at 4℃ for 5 days.The shoot-tips 1.5~2.5 mm in length with one or two leaf primordium of H# Pueraria lobata were cut and precultured at 4℃ for 1 day in MS supplemented with 5% DMSO+5% sucrose.They were dehydrated with 60% and 100% PVS2(PVS2:30% glycerol+15% glycol+15% dimethyl sulfoxide+13.7% sucrose) at 0℃ for 30 minutes, respectively.The shoot-tips were immersed immediately into liquid nitrogen directly and conserved for 24 hours.After rapidly thawing in a water bath at 40℃ for 90 seconds, the shoot-tips were washed two times with MS supplemented with 41.1% sucrose (10 minutes each time), then transferred and re-cultured on the MS medium supplemented with BA 2 mg/L and NAA 1mg/L in dark for 7 days and then in light.The survival rate was up to 60%.The regenerated plantlets of Pueraria lobat were the same as the normal plantlets in morphology. Related Articles | Metrics

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Effects of Basic Medium and Plant Growth Regulator Combination on Multiplication and Differentiation of Rhizome in Cymbidium ensifolium var.susin GAO Li, LI Hong-lin, YANG Bo 2007, 36(04): 13-15.  Abstract ( )   PDF (335KB) ( )   Effects of basic medium and plant growth regulator combination on the multiplication and differentiation of rhizomes of Cymbidium ensifolium var.susin induced from seed germination were studied.The results showed that the optimum medium for multiplication and growth of rhizomes was MS medium, and the best culturing period of multiplication was 40 days.The optimum plant growth regulator combination for differentiation of rhizomes was NAA 0.2 mg/L+6-BA 2.0 mg/L, which regeneration rate of shoots reached 445%. Related Articles | Metrics

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Correlation Analysis Between Photosynthetic Rate and Some Ecological Factors of Populus deltoides CL. ‘Zhongjia 8’ WU Rui-yun 2007, 36(04): 16-19.  Abstract ( )   PDF (195KB) ( )   To analysis the correlation between net photosynthetic rate and ecological factors, using the methods of stepwise regression and analysis of path is much better than correlation analysis of duality variable.By the methods of stepwise regression and analysis of path, this paper studied the diurnal changes of zhongjia8, a crossbreed from Occident Poplar, during early summer and autumn.The results indicated that the diurnal changes of Pn showed a single-peak curve in early summer, the major factor affecting it was PAR (X1), and PAR had extremely significant correlation with Pn.The optimum equation of Pn was attained as follows:Y=17.8271+0.0108 X1-0.2185 X2(multiple correlated coefficient R=0.9954, F=215.4439, significance level p=0.0001, Pn-Y, PAR-X1, RH-X2).But in September, Pn showed a two-peak curve, the major factor affecting it was Ca(X4).The optimum equation was attained as follows:Y=5.2915-0.0030X1+0.3414X3-0.0216X4 (multiple correlated coefficient R=0.8962, F=4.0823, significance level p=0.1391, Pn-Y, PAR-X1, RH-X2, Tleaf-X3, Ca-X4). Related Articles | Metrics

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Comparison on Photosynthetic Characteristics among Three Species of Chimonanthus in Summer and Winter LU Yi-jun, HU Zhong 2007, 36(04): 20-23.  Abstract ( )   PDF (275KB) ( )   The photosynthetic characteristics of Chimonanthus praecox, C. zhejiangensis and C. salicifolius in summer and winter were measured.The transformation of the maximum net photosynthetic rate, light compensation point, light saturation point, the net photosynthesis rate, carboxylation efficiency, stomatal conductance, water use efficiency and the chlorophyll content between in summer and in winter were compared.The results showed that C. praecox, C. zhejiangensis and C. salicifolius all belonged to typical shade-tolerance species and the capacity of shade-tolerance in C. praecox was higher than those of C. zhejiangensis and C. salicifolius, and the capacities of the three species in summer were higher than those in winter. Related Articles | Metrics

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Effects of Extract Solution from the Seed of Jatropha curcas on Seed Germination and Seedling Growth of Crops HU Yuan, ZHAO De-gang 2007, 36(04): 24-26.  Abstract ( )   PDF (155KB) ( )   Jatropha curcas is one of important raw materials of biological diesel oil.The seed germination and growth of rape, fodder grass and wheat treated with the solution extracted from the seeds of Jatropha curcas were investigated to study the effects of mass cultivation of Jatropha curcas on the environment and the growth of plants.The results showed that the water solution from Jatropha curcas seeds reduced the germination percentage of seeds, the length of buds and the length of roots of these three crops.Most alcoholic extracts could reduce the germination percentage of seeds, the length of buds and the length of roots of fodder glass and wheat.But some alcoholic extracts at specific concentration could promote the growth of rape buds. Related Articles | Metrics

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Technology of Aseptic Seeding and in Vitro Propagation of Anota hainanensis PAN Xue-feng, WENG Bao-shi, WANG Xiao-jing 2007, 36(04): 27-29.  Abstract ( )   PDF (313KB) ( )   The techniques for aseptic germination of Anota hainanensis seeds were studied.The results showed that in medium of MS+6-BA 0.1 mg/L, protocorm could form from seeds of Anota hainanensis;1/2 MS supplemented with 6-BA 1.0 mg/L+NAA 0.1 mg/L was suitable for proliferation of protocorm, and the rate of proliferation was 793.3%;the medium of 1/2 MS+IBA 1.0 mg/L+0.2% active carbon+10% banana serum+10% coconut milk gave the best result for rooting of tube plants.When transplanted on coconut bran, the survival rate of tube plant reached 91.4%. Related Articles | Metrics

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Investigation on the Placement Tolerance and Replacement Period of Indoor Ornamentals:A Case Study of Changsha, Hunan Province YAN Bing-wen, YAN Yu-juan 2007, 36(04): 30-32.  Abstract ( )   PDF (149KB) ( )   The essay analyses and studies about 100 species of indoor ornamentals' placement tolerance in Changsha according to their placement tolerance standard, and primarily ensures their replaced period.It can offer scientific reference to choose appropriate ornamental indoor plants. Related Articles | Metrics

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Application of Indigenous Plants to Urban Landscape in Guangzhou WANG Zhong, OUYANG Chan-juan, LUO Yan-yan, WANG Rui-jiang 2007, 36(04): 33-37.  Abstract ( )   PDF (281KB) ( )   Our extensive investigation to the landscape plants cultivated in the urban green-lands of Guangzhou city and our comprehensive assessment to the species constitution revealed that the garden plants were very rich in terms of species number but only small part of plants were highly dominant in terms of species constitution.Moreover, the indigenous plants were poorly applied, on the contrary, the alien plants took up dominant proportion and poisonous plants were also commonly introduced in Guangzhou city.On the basis of our investigation to the wild plants in Guangzhou district, we selected 11 arboreal, 6 shrubby, and 3 herbal indigenous plants with high ornamental and applicable values and introduced their features and uses, so as to provide much valuable reference for the future selection and application of landscape plants in Guangzhou. Related Articles | Metrics

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Ornamental Ground Cover Plant Resources in Xiamen Area and Their Applications in Landscape CHEN Bi-e 2007, 36(04): 38-44.  Abstract ( )   PDF (449KB) ( )   The paper first reported the ornamental ground cover plants included 62 families, 129 genera and 166 species (including varieties) in Xiamen area.Their applications to landscape were also discussed. Related Articles | Metrics

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Studies on the Endangerment Reasons and Conservation Strategies for Dendrobium fimbriatum LIU Qiang, YIN Shou-hua, HUANG Wen, YIN Jian-tao 2007, 36(04): 45-47.  Abstract ( )   PDF (147KB) ( )   Through investigating the biological characteristics of endangered Dendrobium fimbriatum and the current status of its wild resources, we found that the endangerment of Dendrobium fimbriatum was resulting from its own habitats for growth and propagation, and also human destruction as well.Some protection strategies for this species were put forward. Related Articles | Metrics

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Investigation on Vegetation Types of Tongledashan Nature Reserve in Yunan, Guangdong Province XU Yong-fu, YU Xun-lin, ZHU Zhi-ping 2007, 36(04): 48-50,54.  Abstract ( )   PDF (242KB) ( )   Through the outdoor investigation and statistical analysis in Tongledashan Nature Reserve, the vegetation of this reserve could be divided into 5 vegetation types, 8 vegetation subtypes, 15 formations and 21 associations.Based on the distribution rules of Chinese Vegetation, it showed that the main forest type of this reserve is south subtropical broad-leaved evergreen forest, and there also exits warm coniferous forest, warm bamboo forest, south subtropical broad-leaved evergreen shrub and herb in Tongledashan Nature Reserve.Meanwhile, more detailed analysis and description on each vegetation type were also described. Related Articles | Metrics

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Resources of Clematis in Zhejiang Province and Its Utilization Prospect ZHANG Yin-ke, ZHOU Jian-guo, GAO Zhan 2007, 36(04): 51-54.  Abstract ( )   PDF (178KB) ( )   The landscape plant resources are abundant in Zhejiang Province.Being the important ornamental plants and medicinal plants, the Clematis has a great future in development and utilization.Based on the investigation of Clematis, the species of Clematis distributed in Zhejiang province and their distribution range were discussed, and some advices on landscaping application of Clematis were put forward. Related Articles | Metrics

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In vitro Culture and Plantlet Regeneration of Spathe of Anthurium andraenum XU Bin, WANG Guang-dong, GUO Wei-ming, WEN Fang-de 2007, 36(04): 55-55.  Abstract ( )   PDF (88KB) ( )   Related Articles | Metrics

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Studies on Tissue Culture and Plantlet Regeneration of Perilla frutescens HUANG Hai-quan, ZHANG Lei, HUANG Mei-juan 2007, 36(04): 56-56.  Abstract ( )   PDF (89KB) ( )   Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Lilium regale XIN Pei-yao, LUO Si-bao 2007, 36(04): 57-57.  Abstract ( )   PDF (90KB) ( )   Related Articles | Metrics

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The Research Progress of Biotechnology on Bromeliads LIU Hui-chun, PAN Xiao-yun, ZHU Kai-yuan, WANG Wei-yong 2007, 36(04): 58-61.  Abstract ( )   PDF (193KB) ( )   This paper described the recent advances on Bromeliads applications and basic researches.The researches on tissue culture and germplasm preservation were mainly introduced.The paper reported the general situations of genetic transformation and genetic markers on bromeliads.Some problems and perspectives of this field were also discussed. Related Articles | Metrics

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Recent Advances in Phosphorus Acquisition from Non-labile Sources in Plants with Arbuscular Mycorrhizal Interaction LIU Jin-fa, XIA Ren-xue, WANG Ming-yuan, WANG Peng 2007, 36(04): 62-66.  Abstract ( )   PDF (223KB) ( )   Many researches on phosphorus acquisition from non-labile sources in plants with Arbuscular mycorrhizal interaction are reviewed.The metabolism of phosphorus uptake are discussed from the following aspects:expanding the absorption area of plant roots, improving the micro-environment of rhizosphere and accelerating the transport of phosphorous. Related Articles | Metrics

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Advances in Genetic Engineering of Disease Resistance of Capsicum annuum LUO Qi-jun, ZENG Fu-hua 2007, 36(04): 67-71.  Abstract ( )   PDF (221KB) ( )   Capsicum annuum is an important vegetable and usually suffers from diseases caused by virus, bacteria and fungi.A new approach of breeding in Capsicum disease resistance was presented with the developing of genetic engineering.The advances of genetic engineering of disease resistance, mainly on the base of tissue culture and the transgenic strategy, are reviewed in this paper. Related Articles | Metrics

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Study on the Conservation of Orchidaceae HUANG Nuan-ai, HUANG Mian-jia 2007, 36(04): 72-76.  Abstract ( )   PDF (226KB) ( )   Most species of Orchidaceae are critically endangered or rare, and wild orchids all over the world are put in the CITES list, the proportion of which is 90%, it is the flag group in plant conservation.Consequently, it is very important to study and conserve the wild orchids throughout the world from the scientific point of view.This article focuses on the study and conservation of orchids in China as well as foreign countries in recent years. Related Articles | Metrics

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Recent Advances in Germplasm Resources of Eriobotrya and Horticultural Studies on Eriobotrya japonica HONG Lin 2007, 36(04): 77-82.  Abstract ( )   PDF (448KB) ( )   This paper summarizes the resources distribution and taxon of Eriobotrya Lindl.The research advances in the horticultural studies on cultivating, breeding, propagation, tissue culture, physiology, genetics of the common loquat are reviewed. Related Articles | Metrics