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    Research Reports
    Phenology of the Main Landscape Plants in Urban Area of Shenzhen and Their Effects on the Vegetation Landscape
    2013, 42(02): 0-136. 
    Abstract ( )   PDF  
    Phenology showed the different morphology of plant in different of phase, formed the dynamic variety of vegetation landscape. The seasonal aspect variety of whole vegetation landscape could be controlled in a district, contributing to scenic spots program and landscape reconstruction. Based on the investigation and statistics of plant species in the main parks of Shenzhen urban area, phenology of the main landscape plants were observed and noted, finally the suitable phenological characteristics were confirmed, their effects on the vegetation landscape were also analyzed. The advices on the transform of vegetation landscape and selection of landscape plant were presented.
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    Establishment and Optimization of ISSR-PCR Reaction System in Precocious Pyrus pyrifolia
    SONG Yan-chun,LI Yong-yu,CHEN Jian-yan,HUANG Tian-yi,GONG Li,WU Shao-hua
    2013, 42(02): 91-96.  DOI: 10.3969/j.issn.1009-7791.2013.02.001
    Abstract ( )   PDF (586KB) ( )  
    To obtain 7 factors (DNA template dosage, Taq DNA polymerase dosage, primer concentration, dNTP concentration, Mg2+ concentration, annealing temperature and PCR cycles ) of ISSR-PCR Reaction System in Pyrus pyrifolia ‘Cuiguan’, a precocious cultivar of southern China was been used as template. An optimum reaction system was been established. The final total volume was 20 μL, contains 2 μL 10×PCR buffer (Mg2+ free), 60 ng DNA template, 0.5 U Taq DNA polymerase, 1 μmol/L of primer concentration, 90 μmol/L of dNTP concentration, 2.25 mmol/L of Mg2+ concentration. Amplication procedure was as follows: initial-denaturing of 5 min at 94 ℃, followed by 42 cycles of 45 s for denaturing at 94 ℃, at annealing temperature for 45 s and 1 min at 72 ℃, after that 10 min extending at 72 ℃, and then keep at 4 ℃. Use 1.5% of agarose gel electrophoresis to test the genetic polymorphism.
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    Research Reports
    Morphological Variation of Three Species in Pterocypsela Shih and Its Taxonomic Significance
    2013, 42(02): 0-173. 
    Abstract ( )   PDF  
    Based on the concept of population, morphometric and statistical analysis of the distinguishing morphological characters have been carried out on three species of Pterocypsela Shih, and its taxonomic significance has been evaluated. The results show that the character variation amplitude of the shape of leaf blade, the degree of blade split, stem with hairs or not, pod beak length is large and existences interspecific cross, so they can’t be used as identified characteristics. According to the results, we think that there are no prominent differences among P. indica, P. laciniata and P. formosana. It is recommended that P. formosana should be reduced as synonym P. indica like P. laciniata.
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    Cloning and Expression of ZDS Gene from Narcissus tazetta var. chinensis
    ZHONG Xian,JIANG Lin-yu,PAN Dong-ming,PAN Teng-fei,BIAN A-na,
    2013, 42(02): 97-103.  DOI: 10.3969/j.issn.1009-7791.2013.02.002
    Abstract ( )   PDF (667KB) ( )  
    The ZDS gene was cloned from total RNA of flower in Narcissus tazetta var. chinensis by RACE technology. The expression analysis of ZDS gene was predicted with some related software tools, and the expression of ZDS gene in different floral organ of different varieties was analysis with real-time PCR. The sequence analysis indicated that the length of ZDS is 2189 bp, it contains an ORF encoding 499 amino acid residues(Mw=63.6 kDa), as well as 5' non-coding region with 69 bp and 3' non-coding region with 395 bp, named as Ntzds (GenBank accession number EU573238). Compared with ZDSs of other plants, Ntzds amino acid sequence has 97%, 85%, 83% identity with that of Narcissus pseudonarcissus, Vitis vinifera and Citrus unshiu respectively. Expressions of Ntzds in petals and corona from N. tazetta var. chinensis, ‘Minnow’, ‘Fortissimo’ were investigated by real-time qPCR. The results showed that Ntzds gene expression was different in three varieties. The expression level in corona was higher than in petals in same variety; we concluded that the transcription level of Ntzds was developing with the deepening of flower color.
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    Research Reports
    Cystorchis, a Newly Recorded Genus of Orchidaceae from Hainan Island in China
    2013, 42(02): 0-180. 
    Abstract ( )   PDF  
    Cystorchis Blume as a newly recorded genus of Orchidaceae was found in Jianfengling National Nature Reserve of Hainan Island. Hainan Island is the second habitat found in China for the genus. According to the endangered standard of IUCN Red List, Cystorchis aphylla should be protected in situ conservation with a provisional rating of Vulnerable (VU D2).
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    Pharmacognostic Identification of Clematis florida var. plena
    HUANG Ze-hao,ZHANG Yue-ling,SHEN Xian-juan
    2013, 42(02): 104-108.  DOI: 10.3969/j.issn.1009-7791.2013.02.003
    Abstract ( )   PDF (543KB) ( )  
    The roots of Clematis florida var. plena (Ranunculaceae) were usually used as an ethnic drug in Fujian province. In this paper, we described the morphology, pharmaceutical character and microscopic characteristics of this ethnic medicine to identify it and provide foundation for its further study and application.
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    Research Reports
    The Resources of Ornamental Plants in Chirita from Guangdong
    2013, 42(02): 0-140. 
    Abstract ( )   PDF  
    Plants of Gesneriaceae in China with a lot of ornamental species, especially Chirita, have much value to exploit. The resource distribution and ornamental characteristics for Chirita in Guangdong are introduced in detail, and 8 species are screened out because of their higher ornamental promising and value. Finally, the obstacles of explosion of these plants are analyzed.
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    Soil Fertility Characteristics of Road Green-land in Guangzhou
    HU Yan-hui,XIONG Yong-mei,XU Zhi-ping,WU Wei-zhan
    2013, 42(02): 109-112.  DOI: 10.3969/j.issn.1009-7791.2013.02.004
    Abstract ( )   PDF (242KB) ( )  
    According to the sampling research of soil for the green-land of 12 urban roads in Guangzhou by using principal component analysis, the soil fertility characteristics were studied and the dominant factors were screened. The results showed that the soil pH value was 5.52-8.76, in which the ratio of alkaline soil to strong alkaline soil was 78.26%; the EC value and the average value of organic matter were 0.19 mS/cm and 26.52 g/kg, respectively, which led to low water soluble salt and organic nutrient; in addition, available P was at a high level, and next levels were total N and total K, while total P, available N and available K were at low levels. The screening results showed that, the soil pH, organic matter, total N, total P, available N and available P could be selected as target on evaluating soil fertility as main factors, meanwhile, the soil total K and available K could be selected as target on evaluating soil fertility as secondary factor. Eventually, these research results provided a basis for the construction management and post maintenance of urban road green-land.
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    Effects of Soil Salinity on the Quality of Carrot
    TANG Hui-hua,HU Hong-you,YE Ming-xin,JIN Hong,LIN Xian-kun
    2013, 42(02): 113-116.  DOI: 10.3969/j.issn.1009-7791.2013.02.005
    Abstract ( )   PDF (204KB) ( )  
    A soil pot experiment with different levels of saltnity (0, 500,1500, 2000, 2500 mg/kg) were carried out to study the effects of salinity on the quality of carrot. The results showed that controlling certain salinity 500 mg/kg, could improve the quality of carrot. Because the salinity of 500 mg/kg had no effect on the chlorophyll content of carrot, and high salinity caused total chlorophyll content decreased, low salinity was conducive to the promotion of carrot synthesis of vitamin C and soluble protein,soluble sugar content of carrot was decreased by the increasing of salinity levels, Nitrate content in carrots increased at the high salinity ( 2500 mg/kg ) treatment.
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    Research Reports
    Rapid Propagation Technique in vitro of Pseudostellaria heterophylla ‘ZheShen No. 1’
    2013, 42(02): 117-120. 
    Abstract ( )   PDF  
    Leaves and stem segments of Pseudostellaria heterophylla ‘ZheShen No. 1’ were used as explants to study the rapid propagation technique in vitro. The results showed that stems with axillary buds were better for multiple shoot induction. The medium of multiple shoot induction was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 30 g/L. The proliferation medium was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 30-40 g/L. The rooting medium was MS + NAA 0.1-0.2 mg/L + sucrose 30 g/L.
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    Rapid Propagation Technique in vitro of Pseudostellaria heterophylla ‘ZheShen No. 1’
    HONG Xiao-yun,SU Ming-hua,ZHENG Cheng-le,HE En-ming
    2013, 42(02): 117-120.  DOI: 10.3969/j.issn.1009-7791.2013.02.006
    Abstract ( )   PDF (775KB) ( )  
    Leaves and stem segments of Pseudostellaria heterophylla ‘ZheShen No. 1’ were used as explants to study the rapid propagation technique in vitro. The results showed that stems with axillary buds were better for multiple shoot induction. The medium of multiple shoot induction was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 30 g/L. The proliferation medium was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 30-40 g/L. The rooting medium was MS + NAA 0.1-0.2 mg/L + sucrose 30 g/L.
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    Research Reports
    Characteristic Analysis of Ancient Tree Resources in Guangde County, Anhui Province
    2013, 42(02): 146-152. 
    Abstract ( )   PDF  
    There are 923 strains of ancient trees at more than 100 years old in Guangde County, Anhui Province, belonging to 43 species and 1 variety, 38 genera and 24 families, with Liquidambar formosana the largest number, up to 322 individuals, and Ginkgo biloba, Castanopsis sclerophylla, Zelkova schneideriana, Aphananthe aspera, Celtis sinensis, Pistacia chinensis, and Acer buergerianum all also more than 50 strains. Among them, 14 strains are classified as ClassⅠunder state protection, and 30 strains as Class Ⅱ, 879 strains as Class Ⅲ. The number of species and strains in terms of age is roughly in Pyramid-type distribution, with the overall trend is that the age higher, the family, genus, species and strains less, and only 3 strains exceed 700 years old including the oldest 820 years approximately. In these ancient trees, the big arbor species are the most, the middle arbor species secondary, the small arbor and shrub species rarely, and woody lianas the least. The ancient trees mainly distribute in village, wilderness, roadside, riverside and hillside. The ancient trees in village are primary from cultivation and wild origin, and in wilderness, roadside, riverside, hillside, are majorly from wild origin. In the floristic elements of ancient trees in the county, there are 10 tropical genera, accounted for 26.3% of total genera, and 20 temperate genera, accounted for 52.6%, which shows some transition characteristic. The species of East Asia and Chinese endemic are the dominant species evidently, with major relict, ancient and endemic elements. According to the research conclusion of ancient tree resources, the species for afforestation and greening in the future in Guangde County are discussed and suggested.
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    Rhizome Propagation of Cypripedium guttatumu Tube Seedlings
    SUN Jing-liang,DONG Xiang-nan,FU Tian-si,YANG Wen-xin,Jiang Chang-yang
    2013, 42(02): 121-123.  DOI: 10.3969/j.issn.1009-7791.2013.02.007
    Abstract ( )   PDF (225KB) ( )  
    In order to protect germplasm and realize cultivation, the tissue culture method was used to advance seedlings culture, rhizome subculture, seedling cuttings and planting research, with the rhizome of Cypripedium guttatumu as materials. The results showed that 1/2MS + sucrose 16 g/L + ABT2 10 mg/L + IAA 0.4 mg/L was the ideal medium for tube seedlings and the cinder was the ideal matrix for the subculture of tube seedlings. The cutting survival rate on greenhouse seedbed was 96.5%, which was improved by 2.2 times than transplanting. The planting survival rate of tube seedlings attained 96.8%, and the tube seedlings also maintained all botany characteristics of wild C. guttatumu.
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    Effect of Activated Carbon on the Seed Germination and Seedling Growth of Wheat Variety Yannong 19
    YIN Xiang-bo,LIU Chen,WANG Zhi-li,MA Yun-fei
    2013, 42(02): 124-126.  DOI: 10.3969/j.issn.1009-7791.2013.02.008
    Abstract ( )   PDF (296KB) ( )  
    With garden soil for basic training materials, the germination and seedlings growth of wheat variety Yannong 19 were investigated under the conditions of different content of activated carbon. The activated carbon 0.0 (CK), 0.5, 1.0, 1.5, 2.0 g mixed evenly into 150 g of garden soil and were used to culture the seed of wheat variety Yannong 19. After germination, the wheat germination rate, seedling traits indicators of height, root length, fresh weight, and soluble sugar and chlorophyll content were measured. The results showed that within the experimental range, with the increase of the application rate of activated carbon, the wheat root length, soluble sugar content and chlorophyll content were rising firstly and then falling, but seedling height and germination rate continued to increase. So the appropriate activated carbon mixed in garden soil can effectively promote the growth of wheat.
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    An Introduction Experiment of Endangered Species Bretschneidera sinensis Afforestation at Different Altitudes
    XIONG Zi-hua
    2013, 42(02): 127-130.  DOI: 10.3969/j.issn.1009-7791.2013.02.009
    Abstract ( )   PDF (228KB) ( )  
    Afforestation trial on 4-year-old Bretschneidera sinensis were conduted at different altitudes in Jianning county, Fujian province. And the net growth of tree height and diameter on root collar (DRC) were investigated after 4 to 8 years planting. The results showed that there were insignificant differences in afforestation survival rate among the B. sinensis seedlings planted at different altitudes. But the increment of tree height and DRC among those at different altitudes differed significantly. The increments of tree height at altitudes 350, 660, 750 m were 72, 164, 162 cm, respectively. Meanwhile, the growth mass of DRC at altitudes 350, 660, 750 m were 0.93, 1.53, 1.83 cm respectively. In a word, the net growth of tree height and DRC were increased with a rise in altitude for afforestation ranging from 350-750 m. Consequently altitude was one of the most important factors for B. sinensis seedlings afforestation.
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    Phenology of the Main Landscape Plants in Urban Area of Shenzhen and Their Effects on the Vegetation Landscape
    LI Wei,HUANG Zheng,SU Dan-ping,HE Tao
    2013, 42(02): 131-136.  DOI: 10.3969/j.issn.1009-7791.2013.02.010
    Abstract ( )   PDF (385KB) ( )  
    Phenology showed the different morphology of plant in different of phase, formed the dynamic variety of vegetation landscape. The seasonal aspect variety of whole vegetation landscape could be controlled in a district, contributing to scenic spots program and landscape reconstruction. Based on the investigation and statistics of plant species in the main parks of Shenzhen urban area, phenology of the main landscape plants were observed and noted, finally the suitable phenological characteristics were confirmed, their effects on the vegetation landscape were also analyzed. The advices on the transform of vegetation landscape and selection of landscape plant were presented.
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    Investigation on Resources of Ornamental Plants in Chirita from Guangdong
    YANG Li-hua,ZHENG Zhou-xiang,ZHOU Ji-gang,XU Ping,SHU Xia-zhu
    2013, 42(02): 137-140.  DOI: 10.3969/j.issn.1009-7791.2013.02.011
    Abstract ( )   PDF (241KB) ( )  
    Plants of Gesneriaceae in China with a lot of ornamental species, especially Chirita, have much value to exploit. The resource distribution and ornamental characteristics for Chirita in Guangdong are introduced in detail, and 8 species are screened out because of their higher ornamental promising and value. Finally, the obstacles of explosion of these plants are analyzed.
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    Damage Status and Invasive Dynamic of an Alien Species Mikania micrantha in Hainan Island
    PENG Zong-bo,LI Jian-hua,JIANG Ying,WANG Chun-yan,JIANG Ju-sheng
    2013, 42(02): 141-145.  DOI: 10.3969/j.issn.1009-7791.2013.02.012
    Abstract ( )   PDF (485KB) ( )  
    For comprehensive understand the damage status and invasive dynamics of Mikania micrantha in Hainan island, and provide scientific basis for effective prevention and control on it, invasion of M. micrantha in Hainan island were investigated, the invasion dynamics were analyzed, and its distribution was predicted. Since the first appearance in Haikou and Wenchang city in 2003, M. micrantha has invaded into more than 10 cities in Hainan island after a few years. It competed with the local species for resources, influence the agriculture and forestry production. For its strong reproductive capacity, rapid growth, good adaptability and other characteristics, it has the potential ability to spread overall of Hainan island by forecast. Therefore, we must adopt comprehensive method of prevention and control, and effectively reduce the invasion and losses caused by it.
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    Characteristic Analysis of Ancient Tree Resources in Guangde County, Anhui Province
    FU Le-yi
    2013, 42(02): 146-152.  DOI: 10.3969/j.issn.1009-7791.2013.02.013
    Abstract ( )   PDF (326KB) ( )  
    There are 923 strains of ancient trees at more than 100 years old in Guangde County, Anhui Province, belonging to 43 species and 1 variety, 38 genera and 24 families, with Liquidambar formosana the largest number, up to 322 individuals, and Ginkgo biloba, Castanopsis sclerophylla, Zelkova schneideriana, Aphananthe aspera, Celtis sinensis, Pistacia chinensis, and Acer buergerianum all also more than 50 strains. Among them, 14 strains are classified as ClassⅠunder state protection, and 30 strains as Class Ⅱ, 879 strains as Class Ⅲ. The number of species and strains in terms of age is roughly in Pyramid-type distribution, with the overall trend is that the age higher, the family, genus, species and strains less, and only 3 strains exceed 700 years old including the oldest 820 years approximately. In these ancient trees, the big arbor species are the most, the middle arbor species secondary, the small arbor and shrub species rarely, and woody lianas the least. The ancient trees mainly distribute in village, wilderness, roadside, riverside and hillside. The ancient trees in village are primary from cultivation and wild origin, and in wilderness, roadside, riverside, hillside, are majorly from wild origin. In the floristic elements of ancient trees in the county, there are 10 tropical genera, accounted for 26.3% of total genera, and 20 temperate genera, accounted for 52.6%, which shows some transition characteristic. The species of East Asia and Chinese endemic are the dominant species evidently, with major relict, ancient and endemic elements. According to the research conclusion of ancient tree resources, the species for afforestation and greening in the future in Guangde County are discussed and suggested.
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    Exploitation and Utilization of Substituting Tea Plant Resources in Youxi County, Fujian Province
    CHEN Mao-jing
    2013, 42(02): 153-159.  DOI: 10.3969/j.issn.1009-7791.2013.02.014
    Abstract ( )   PDF (347KB) ( )  
    The substituting tea plants in Youxi county, Fujian province, were investigated through a combination method of survey on important wild fields, collecting materials and interviewing. The result showed that there were substituting tea plants 48 species (including varieties) belonging to 57 genera in 32 families. The substituting tea plants with great development and utilization value were Houttuynia cordata, Sarcandra glabra, Reynoutria japonica, Distylium myricoides, Ampelopsis grossedentata, Ginkgo biloba, Imperata cylindrica var. major, Choerospondias axillaris and some species of Lonicera, Ilex. The type of life, the parts of substituting tea, care type, efficacy, the tastes characteristics and the altitude distribution about every species of substituting tea plants were listed. The suggestions about the development and utilization of those plants were put forward.
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    Resources of Wild Ilex Plants in Provincial Nature Reserve Jiufu Mountain, Fujian Province
    CHEN Tie-fang
    2013, 42(02): 160-164.  DOI: 10.3969/j.issn.1009-7791.2013.02.015
    Abstract ( )   PDF (277KB) ( )  
    The resources of wild Ilex plants in Provincial Nature Reserve-Jiufu Mountain, Fujian Province were investigated. The results showed that there were 19 kinds of wild Ilex Plants in this area in total. The wild Ilex plants with great development and utilization value are I. pubescens, I. latifolia, I. asprella, I. theicarpa, I. centrochinensis, I. kwangtungensis, I. suaveolens, I. micrococca. The life form, life environment, florescence, colors of the flowers, fruiting, colors of the fruit, fruit diameter, medicinal parts, resource distribution and storage, the ways of development and utilization about wild Ilex plants in this area were analyzed. At the same time, countermeasures about the protection and the development and utilization of the wild Ilex plants were put forward.
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    Flora of Spermatophyte in Jiufu Mountain Natural Reserve of Youxi County, Fujian Province
    LIN Sheng jian
    2013, 42(02): 165-168.  DOI: 10.3969/j.issn.1009-7791.2013.02.016
    Abstract ( )   PDF (221KB) ( )  
    Jiufu Mountain provincial natural reserve area is located in the subtropical forest ecosystem region, there are 992 species (including varieties and forma), of 491 genera, within 137 families of spermatophyte. The survey results show that the floristic elements are complex, rich in monotypical and oligotypical genus. The flora is ancient and interim clearly. According to the analysis on the geographic component of the flora, it is concluded that the nature of Jiufu Mountain flora is a typical of subtropical characteristics and transition to tropical one.
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    Morphological Variation of Three Species in Pterocypsela Shih and Its Taxonomic Significanc
    QIU Sheng,LAI Meng-meng,LING Wen-ting,LIN Zuo-zhen,ZHENG Kang,DING Bing-yang
    2013, 42(02): 169-173.  DOI: 10.3969/j.issn.1009-7791.2013.02.017
    Abstract ( )   PDF (262KB) ( )  
    Based on the concept of population, morphometric and statistical analysis of the distinguishing morphological characters have been carried out on three species of Pterocypsela Shih, and its taxonomic significance has been evaluated. The results show that the character variation amplitude of the shape of leaf blade, the degree of blade split, stem with hairs or not, pod beak length is large and existences interspecific cross, so they can’t be used as identified characteristics. According to the results, we think that there are no prominent differences among P. indica, P. laciniata and P. formosana. It is recommended that P. formosana should be reduced as synonym P. indica like P. laciniata.
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    New Materials of Plants in Fujian Province (Ⅰ)
    JIANG Qiu-yi,HOU Xue-liang,WANG Wen-qing,ZHANG Yi-hui
    2013, 42(02): 174-176.  DOI: 10.3969/j.issn.1009-7791.2013.02.018
    Abstract ( )   PDF (291KB) ( )  
    During the course of investigating the plants in Quanzhou bay, four newly recorded species and one newly recorded varieties of Angiosperm were found. They are Suaeda salsa, Crotalaria nana var. patula, Phyllanthus arenarius, Hedyotis strigulosa and Eragrostis fauriei. The voucher specimens are deposited in the Herbarium of Xiamen University (AU).
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    Cystorchis, a Newly Recorded Genus of Orchidaceae from Hainan Island in China
    YANG Hai-jun,XU Han,CUI Da-fang,LI Yi-de
    2013, 42(02): 177-180.  DOI: 10.3969/j.issn.1009-7791.2013.02.019
    Abstract ( )   PDF (336KB) ( )  
    Cystorchis Blume as a newly recorded genus of Orchidaceae was found in Jianfengling National Nature Reserve of Hainan Island. Hainan Island is the second habitat found in China for the genus. According to the endangered standard of IUCN Red List, Cystorchis aphylla should be protected in situ conservation with a provisional rating of Vulnerable (VU D2).
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    Symplocos botryantha Franch., a New Record of Wild Plants in Fujian Province
    PENG Shao-yun
    2013, 42(02): 181-181.  DOI: 10.3969/j.issn.1009-7791.2013.02.020
    Abstract ( )   PDF (223KB) ( )  
    Symplocos botryantha Franch., a wild plant, was newly recorded in Fujian province. The voucher specimens were deposited in Herbarium of Longyan Forestry Science Research Institute.
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    Research Progress in the Variations of Caffeine During the Post-Fermentation of Pu-erh Tea
    CHAI Jie,GUO Wei,YANG Chao,MA Cun-qiang,REN Xiao-ying,ZHOU Bin-xing
    2013, 42(02): 182-186.  DOI: 10.3969/j.issn.1009-7791.2013.02.021
    Abstract ( )   PDF (275KB) ( )  
    The post-fermentation processing technology of Pu-erh tea (ripe tea) is the core procedure to form the special feature. Caffeine (1,3,7-Trimethyl xanthine) as the major flavour and physiological active ingredient is one of abundant biochemical components, which has various efficacies, such as promoting excitement, stimulating cardiac, detoxication, diuresis, aid to digestion and so on. Recent years, majority of researches have demonstrated that the content of caffeine shows the tendency of addition during the post-fermentation process. This paper would systematically summarize current researches on the changes of caffeine in the post-fermentation process and expect to provide scientific foundations for caffeine theory about Pu-erh tea.
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