大叶藤黄种子的愈伤组织及丛生芽诱导

doi:10.3969/j.issn.1009-7791.2024.03.003

亚热带植物科学 ›› 2024, Vol. 53 ›› Issue (3): 207-213.DOI: 10.3969/j.issn.1009-7791.2024.03.003

大叶藤黄种子的愈伤组织及丛生芽诱导

杨兰^1,2，安雪姣¹，陈利钢¹，文彬^1*

(1. 中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室，云南勐腊 666303；2. 中国科学院大学，北京 100049)

收稿日期:2024-04-06 接受日期:2024-05-14 出版日期:2024-06-30 发布日期:2024-08-23
通讯作者: 文彬
基金资助:
国家自然科学基金(31971573)

Induction of Callus and Adventitious Buds of Garcinia xanthochymus Seeds

YANG Lan^1,2, AN Xue-jiao¹, CHEN Li-gang¹, WEN Bin^1*

(1. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, Yunnan China; 2. University of Chinese Academy of Sciences, Beijing 100049, China)

Received:2024-04-06 Accepted:2024-05-14 Online:2024-06-30 Published:2024-08-23
Contact: WEN Bin

摘要/Abstract

摘要： 大叶藤黄Garcinia xanthochymus种子具有全能萌发的特点，但其生理基础仍然不明确。为了研究其再生生长特性，分别以大叶藤黄成熟和未成熟种子的切片为外植体，MS为基本培养基，探讨添加外源NAA、TDZ、IAA、6-BA、2,4-D 等生长调节物质诱导愈伤组织和不定芽的效果。结果表明：(1) 愈伤组织的发生主要取决于种子是否成熟。取自成熟种子的外植体，即使不添加外源生长调节物质，诱导率也超过80%；而取自未成熟种子的外植体，仅TDZ (0.25～3 mg·L^–1)和6-BA (1.5～2 mg·L^–1)有效，且发生率低于40%。(2) 仅6-BA可稳定诱导生成不定芽，最佳方案为取成熟种子外植体在含5.5 mg·L^–1 6-BA的MS培养基上培养诱导，其发生率可达73%，外植体的平均不定芽发生数量为5.07个。该研究结果进一步证明大叶藤黄种子具有强大的再生生长能力，同时所研究的幼苗再生方案也为大叶藤黄无性繁育提供了可靠途径。

关键词: 藤黄型萌发, 全能型萌发, 种子成熟度, 组织培养, 植物生长调节剂

Abstract: Garcinia xanthochymus seeds exhibit the characteristic of totipotent germination, yet therein physiological basis remains unclear. To investigate the regenerative growth characteristics, explants from mature and immature seeds of G. xanthochymus were utilized to examine the impacts of five hormones and growth regulators (NAA, TDZ, IAA, 6-BA, and 2,4-D) on callus induction and adventitious bud formation, with MS as basic media. The results indicated that: (1) Callus formation primarily depended on the maturity of the seeds. Explants derived from mature seeds exhibited induction rates exceeding 80% even without exogenous growth regulators, while explants sourced from immature seeds only responded to TDZ (0.25–3 mg·L^–1) and 6-BA (1.5–2 mg·L^–1) with a callus induction rate below 40%. (2) Only 6-BA induced adventitious buds steadily in this study, with the optimal approach being the cultivation of explants from mature seeds on MS medium supplemented with 5.5 mg·L^–1 6-BA, resulting in a 73% incidence rate and an average of 5.07 adventitious shoots each explant. These findings further validated the strong regenerative growth potential of G. xanthochymus seeds, and the investigated plantlet regeneration protocol provided a reliable method for asexual propagation in G. xanthochymus.

Key words: garcinia-type germination, totipotent germination, seed maturity, tissue culture, plant growth regulators

中图分类号:

Q945

杨兰, 安雪姣, 陈利钢, 文彬. 大叶藤黄种子的愈伤组织及丛生芽诱导[J]. 亚热带植物科学, 2024, 53(3): 207-213.

YANG Lan, AN Xue-jiao, CHEN Li-gang, WEN Bin. Induction of Callus and Adventitious Buds of Garcinia xanthochymus Seeds[J]. Subtropical Plant Science, 2024, 53(3): 207-213.

参考文献

[1] Wu Z Y, Raven P H, Hong D Y. Flora of China (Vol.13) (Clusiaceae) [M]. Beijing: Science Press & St. Louis: Missouri Botanical Garden Press, 2007: 1–32.

[2] Yapwattanaphun C, Subhadrabandhu S, Sugiura A, Yonemori K, Utsunomiya N. Utilization of some Garcinia species in Thailand [C]. Proceeding of International Symposium on Tropical and Subtropical Fruits, 2002: 563–570.

[3] Kalia R K, Malik S K, Chaudhury R. In vitro morphogenetic studies on three apomictic species of Garcinia [J]. Journal of Plant Biochemistry and Biotechnology, 2012, 21: 279–285.

[4] Joshi G, Kumar A N A, Gowda B, Srinivasa Y B. Production of supernumerary plants from seed fragments of Garcinia gummi-gutta: evolutionary implications of mammalian frugivory [J]. Current Science, 2006, 91: 372–376.

[5] Wang Z Y, Cao L, Yan C, Niu Y D, Chong K, Zhang Z B. Cloning capacity helps seeds of Garcinia xanthochymus counter animal predation [J]. Ecology and Evolution, 2021, 11(18): 12639–12650.

[6] Gyimah A. Effect of pretreatment methods on germination of Garcinia kola Heckel seeds [J]. Ghana Journal of Forestry, 2000, 9: 39–44.

[7] Mohan S, Parthasarathy U, Babu K N. In vitro and in vivo adventitious bud differentiation from mature seeds of three Garcinia spp. [J]. Indian Journal of Natural Products and Resources, 2012, 3:65–72.

[8] Thengane S R, Deodhar S R, Bhosle S V, Rawal S K. Direct somatic embryogenesis and plant regeneration in Garcinia indica Choiss [J]. Current Science, 2022: 1074–1078.

[9] Thengane S R, Deodhar S R, Bhosle S V, Rawal S K. Repetitive somatic embryogenesis and plant regeneration in Garcinia indica Choiss [J]. In Vitro Cellular & Developmental Biology-Plant, 2006, 42: 256–261.

[10] Qosim W A, Purwanto R, Wattimena G A. Comparison of adventitious shoot formation of Garcinia mangostana via embryogenesis and direct organogenesis [J]. Asian Journal of Plant Sciences, 2015, 14(2): 78–83.

[11] Baskaran M, Krishnan S. High frequency plant regeneration from the mature seeds of Garcinia indica [J]. Biologia Plantarum, 2011, 55: 554–558.

[12] Sirchi M H, Kadir M A, Aziz M A, Rashid A A, Rafat A, Javadi M B. Amelioration of mangosteen micro propagation through leaf and seed segments (Garcinia mangostana L.) [J]. African Journal of Biotechnology, 2008, 7(12): 2025.

[13] Malik S K, Chaudhury R, Kalia R K. Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species [J]. Scientia Horticulturae, 2005, 106(4): 539–553.

[14] Huang L C, Huang B L, Wang C H, Kuo C I, Murashige T. Developing an improved in vitro propagation system for slow-growing species using Garcinia mangostana L. (mangosteen) [J]. In Vitro Cellular & Developmental Biology-Plant, 2000, 36: 501–504.

[15] Malik S K, Chaudhury R, Abraham Z. Seed morphology and germination characteristics in three Garcinia species [J]. Seed Science and Technology, 2005, 33(3): 595–604.

[16] Sparta A, Triatminingsih R, Joni Z, Nofiarli. The effect of IAA and BAP on the increase of shoot multiplication of mangosteen (Garcinia mangostana L.) through in vitro culture [C]. Proceeding International Conference on Tropical Horticulture 2013: 549–553.

[17] Amente G, Chimdessa E. Control of browning in plant tissue culture: A review [J]. Journal of Scientific Agriculture, 2021, 5(1): 67–71.

[18] Wen B, Wang R. Pretreatment incubation for culture and cryopreservation of Sabal embryos [J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2010, 102: 237–243.

[19] Noor N M, Aizat W M, Hussin K, Rohani E R. Seed characteristics and germination properties of four Garcinia (Clusiaceae) fruit species [J]. Fruits, 2016, 71(4): 199–207.

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大叶藤黄种子的愈伤组织及丛生芽诱导

Induction of Callus and Adventitious Buds of Garcinia xanthochymus Seeds

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