Abstract: The optimization of culture medium for adventitious buds proliferation of Anoectochilus formosanus were studied by orthogonal design L9(34), using the stems of test-tube seedling as explants. The results showed that the basic medium, 6-BA, NAA, sucrose had very significant effect on adventitious bud proliferation culture. The effect for proliferation culture was in the order of sucrose＞the basic medium＞6-BA＞NAA, the optimizing medium for proliferation culture was 1/2MS + 6-BA 3.0 mg/L + NAA 0.6 mg/L + sucrose 10 g/L.

Key words: Anoectochilus formosanus, proliferation, orthogonal design

摘要： 以台湾金线莲试管苗茎段为外植体，应用L9（34）正交试验设计优选不定芽增殖培养基，结果表明，基本培养基、6-BA、NAA、蔗糖对不定芽增殖培养均有极显著影响，其作用表现为：蔗糖＞基本培养基＞6-BA＞NAA，最适宜的增殖培养基为1/2MS＋6-BA 3.0 mg/L＋NAA 0.6 mg/L＋蔗糖10 g/L。

关键词: 台湾金线莲, 增殖培养, 正交设计