Abstract: The test for passing domestication culture of Oncidium adventitious buds from PLBs showed that taking the modified 1 medium as basic medium supplemented with 100g/L coconut milk for organic additive and no plant-growth regulator were favorable for the PLBs regeneration and could promote the adventitious buds to grow healthy and good uniformity. According to the test of culture medium, organic additive, the amount of activated charcoal, illumination intensity, container and the altitude of the plantlet to fixing into the container, esc, all which relating to the culture elements of PLBs rhizogenesis plantlet strengthening were screened. Results showed that, all plantlets growing from modified 1 medium with 100g/L banana mud, 1g/L activated charcoal, no plant-growth regulator, under the strong light (2 000~3 000 lx), which promoting the growth of plantlets had better performance in each growing index, and they were favorable for the survival of the later domestication. Results showed that also the plants growing in the triangular flask had highest inoculation rate, the lowest contamination rate and better growth index. In the altitude 3 cm of the plantlet to fixing into the container, the efficiency of factory micropropagation of Oncidium was improved.

Key words: Oncidium, tissue culture, plantlet

摘要： 文心兰原球茎分化苗过渡驯化培养试验表明,改良1号+椰子汁100g/L的培养基有利于原球茎分化成苗,能促进分化苗生长健壮、整齐度好。壮苗与生根培养试验表明,以改良1号为基本培养基,加入香蕉泥100g/L、活性炭1g/L,在较强光照(2 000~3 000 lx)下培养,植株各项生长指标表现较好,有利于后期炼苗成活;以三角瓶为定瓶容器培养,植株接种效率最高、污染率最低;选取3cm高的植株定瓶,可显著提高文心兰工厂化组培育苗的工作效率。

关键词: 文心兰, 组织培养, 试管苗