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Subtropical Plant Science

Subtropical Plant Science ›› 2009, Vol. 38 ›› Issue (02): 15-18.DOI: 10.3969/j.issn.1009-7791.2009.02.004

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Influences of PPO Inhibitors on Browning in Phalaenopsis Explants

CHEN Dong-yin1, LAI Yan-yan1, XU Chuan-jun1,2, LI Ling1   

  1. 1. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, Guangdong China;
    2. Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China
  • Received:2009-01-19 Online:2009-06-10 Published:2009-06-10

多酚氧化酶抑制剂对蝴蝶兰叶外植体褐变的影响

陈冬茵1,赖艳艳1,许传俊1,2,李玲1   

  1. 1. 华南师范大学 生命科学学院, 广东省植物发育生物工程重点实验室, 广东 广州 510631;
    2. 福建省亚热带植物研究所, 福建 厦门 361006
  • 作者简介:陈冬茵(1987-),女,广东东莞人,本科生。
  • 基金资助:

    国家大学生创新性实验计划项目(081057411);华南师范大学2008年度大学生创新性实验项目资助

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Abstract: The effects of Polyphenol oxidase (PPO) activity inhibitors supplemented to reaction buffer at different concentrations showed that ascorbate acid and Cys complete inhibited PPO activity at 0.5 mmol/L. Adding PPO activity inhibitors to media at different concentrations, the results showed that 300 mg/L citric acid and 100-200mg/L NaHSO3 could reduce the browning of Phalaenopsis explants. Soaking explants with PPO activity inhibitors, it showed that 50 mg/L ascorbate acid gave the best result for browning inhibition and PPO activity was lower than that of control before explants browning happened.

Key words: Phalaenopsis, explants, browning, polyphenol oxidase, inhibitor

摘要： 将多酚氧化酶(PPO)抑制剂添加到酶反应液中,抗坏血酸和半胱氨酸在0.5mmol/L就完全抑制蝴蝶兰PPO活性。300mg/L柠檬酸和100~200mg/L亚硫酸氢钠分别添加到培养基中,可使蝴蝶兰外植体褐变程度降低;采用抑制剂浸泡处理外植体,对外植体褐变抑制效果最好的为50mg/L抗坏血酸,外植体在褐变发生前PPO活性低于对照。

关键词: 蝴蝶兰, 外植体, 褐变, 多酚氧化酶, 抑制剂

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