Abstract: Through the bacteriostatic test of extract from Eclipta prostrata, one of medical plants in the west of Hunan, the results are as follows. (1) The antibacterial substance only exists in the petiole and rhizome. (2) The minimal inhibitory concentration of the extract on Staphylococcus aureus, Escheichia coli, Bacillus subtilis, Micrococcus luteus are 0.6, 2.0, 0.8, 1.0 g/mL respectively; the minimal bactericidal concentration all are 1.0 g/mL; but it is ineffective on Aspergillus niger. (3) In order to get the extract that has the optimum antibacterial effect, the solvent should be alkaline 65% ethyl alcohol. The bacteriostatic test by different polar solvent shows that the antibacterial substance easily dissolved in non-polar solvent such as petroleum ether, ethyl ether. (4) The extract may be flavone substance.

Key words: Eclipta prostrate, extract, antibacterial activity, solvent, soxhlet extraction, ultrasonic extraction

摘要： 对药用植物墨旱莲Eclipta prostrata提取物的抑菌活性物质进行研究。结果表明：（1）通过不同部位提取物的抑菌试验，发现只有叶和根茎含有抑菌活性物质；（2）墨旱莲提取物对金黄色葡萄球菌、大肠杆菌、枯草杆菌、藤黄球菌的最低抑菌浓度分别为0.6、2.0、0.8、1.0 g/mL，最小杀菌浓度均为1.0 g/mL，对白色念珠菌有很强的抑菌作用，但对黑曲霉无效；（3）通过不同极性溶剂的抑菌试验表明，抑细菌活性物质易溶于石油醚、乙醚等非极性溶剂，具有最佳抑细菌效果的提取溶剂为偏碱性的65%乙醇溶液；（4）墨旱莲的抑菌活性物质可能为黄酮类物质。

关键词: 墨旱莲, 提取物, 抑菌活性, 溶剂, 索氏提取, 超声提取