Abstract: With citric acid, sodium pyruvate, sodium acetate, L-tyrosine, L-phenylalanine, lysine as precursors in tissue culture of Tripterygium wilfordii, the rooting of explants and the content of triptolide was determined after 30 d. The results showed that 3.0 mg稬-1 pyruvic acid in illumination condition and 1.0 mg稬-1 sodium acetate in dark condition could get the maximum amount of adventitious roots, while 1.0 mg稬-1 lysine in illumination condition and 1.0 mg稬-1 tyrosine in dark condition could get the highest concentration of triptolide.

Key words: precursor substance, Tripterygium wilfordii, tissue culture, adventitious root induction, triptolide content

摘要： 在雷公藤Tripterygium wilfordii组织培养生根培养阶段以柠檬酸、丙酮酸钠、乙酸钠、L-酪氨酸、L-苯丙氨酸、赖氨酸为前体物质，添加到培养基中，培养30 d后，观测外植体生根情况并测定甲素含量。结果表明，丙酮酸钠3.0 mg·L-1在光照条件及乙酸钠1.0 mg·L-1在黑暗条件培养中可获得最大的不定根量，赖氨酸1.0 mg·L-1在光照条件及L-酪氨酸1.0 mg·L-1在黑暗条件中培养可获得较高含量甲素。

关键词: 前体物质, 雷公藤, 组织培养, 不定根诱导, 甲素含量