An Efficient Plant DNA Extraction and PCR Amplification System

doi:10.3969/j.issn.1009-7791.2018.03.002

Subtropical Plant Science ›› 2018, Vol. 47 ›› Issue (03): 207-210.DOI: 10.3969/j.issn.1009-7791.2018.03.002

• Plant physiology, biochemistry and molecular biology • Previous Articles Next Articles

An Efficient Plant DNA Extraction and PCR Amplification System

XIAO Shuai, LI Lin-xia, LI Hai-ou

(1.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, Hunan China; 2.Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Changsha 410128, Hunan China)

Received:2018-08-17 Revised:2018-09-05 Online:2018-09-30 Published:2018-09-30
Contact: LI Hai-ou

一种高效的植物DNA提取和PCR扩增体系建立

肖帅，李鳞霞，李海鸥

(1.湖南农业大学生物科学技术学院，湖南长沙 410128；2.湖南农业大学植物激素与生长发育湖南省重点实验室，湖南长沙 410128)

通讯作者: 李海鸥
作者简介:肖帅，硕士研究生，从事植物分子生物学研究；李鳞霞，硕士研究生，从事水稻育种研究。
基金资助:
湖南省自然科学基金项目(2016JJ2065)

Abstract

Abstract: A simple and efficient system of plant DNA extraction and PCR amplification was established. The system only need one step to extract DNA, and good amplification results of target genes could be obtained by prolongation PCR predenaturation time. The system had strong experimental stability and universal species applicability. It would greatly improve the efficiency of plant molecular biology experiment and plant breeding experiment based on molecular marker, and decrease scientific research cost.

Key words: DNA extraction, PCR amplification, plant

摘要： 报道一种高效简易的植物DNA提取和PCR扩增体系。该体系仅需一步即可提取DNA，扩增时延长PCR预变性时间便能获得较好的目的基因扩增结果。本体系具有较高的实验稳定性和较好的物种适用性，将大大提高植物分子生物学实验和基于分子标记辅助的植物育种实验效率，节省科研成本。

关键词: DNA提取, PCR扩增, 植物

CLC Number:

Q943.2

XIAO Shuai, LI Lin-xia, LI Hai-ou. An Efficient Plant DNA Extraction and PCR Amplification System[J]. Subtropical Plant Science, 2018, 47(03): 207-210.

肖帅，李鳞霞，李海鸥. 一种高效的植物DNA提取和PCR扩增体系建立[J]. 亚热带植物科学, 2018, 47(03): 207-210.

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An Efficient Plant DNA Extraction and PCR Amplification System

一种高效的植物DNA提取和PCR扩增体系建立

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