Subtropical Plant Science ›› 2019, Vol. 48 ›› Issue (02): 201-203.DOI: 10.3969/j.issn.1009-7791.2019.02.019

• Plant protection and tissue culture • Previous Articles     Next Articles

Studies on Browning in Tissue Culture of Polygonatum kingianum

SU Juan, ZHANG Wan-qiao, WANG Xiao, LI Kun-hua, HAN Dan, YANG Dan, YUE Jian   

  1. (Yunnan Shanlihong Biotechnology Co. Ltd., Kunming 650200, Yunnan China)
  • Received:2019-01-19 Revised:2019-04-11 Online:2019-06-27 Published:2019-06-27
  • Contact: YUE Jian

滇黄精组培褐化影响因素研究

苏娟,张万巧,王晓,李昆华,韩郸,杨丹,岳健   

  1. (云南山里红生物科技有限公司,云南 昆明 650200)
  • 通讯作者: 岳健
  • 作者简介:苏娟,硕士,从事植物组织培养研究。
  • 基金资助:
    云南山里红生物科技有限公司滇黄精基础研究专项基金

Abstract: In this study, the stem buds of Polygonatum kingianum were used as explants, and the factors affecting browning during tissue culture (sterilization method, basic medium and culture temperature) were discussed. The results showed that the anti-browning effect was best when the surface was treated with 75% ethanol for 30 s and then with NaClO 12 min+HgCl2 8 min. The 1/2MS basic medium was used to effectively inhibit the browning; the low temperature environment (20 ℃) could further reduce the incidence of browning.

Key words: Polygonatum kingianum, rhizome bud, tissue culture, browning

摘要: 以滇黄精Polygonatum kingianum块茎芽为外植体,对组培过程中褐化的影响因素(灭菌方式、基本培养基及培养温度)进行探讨。结果表明,先经过75%乙醇处理30 s消毒表面,再用NaClO 12 min+HgCl2 8 min消毒的抗褐化效果最好;选用1/2MS基本培养基能有效抑制褐化;低温环境(20 ℃)能进一步降低褐化发生率。

关键词: 滇黄精, 块茎芽, 组织培养, 褐化

CLC Number: