Abstract: The compounds and diurnal variation of floral scent emitted from Dendrobium primulinum were studied in this paper. The floral scent components of D. primulinum flowers were detected and analyzed at six time points (6:00, 9:00, 12:00, 15:00, 18:00, 21:00) in one day by static headspace combined with GC-MS. The results showed that the ranking of total floral scent emitted was 35.26% at 6:00 > 22.78% at 9:00 > 19.35% at 12:00 > 7.86% at 21:00 > 7.59% at 15:00 >7.17% at 18:00. 202 components were detected, including esters, terpenes, alcohols, aldehydes, alkylsk, et al., of which 93 were at 6:00, 87 were at 9:00, 68 were at 12:00, 61 were at 15:00, 63 were at 18:00, and 65 were at 21:00. Twenty-one common compounds at each time points. The floral scent emitted from D. primulinum was highest at 6:00 and lowest at 18:00. The main floral scent component was octyl acetate.

Key words: Dendrobium primulinum, floral scent, diurnal variation, GC-MS

摘要： 采用静态顶空气相色谱-质谱(GC-MS)联用技术研究报春石斛(Dendrobium primulinum)花香气成分及香气释放的日变化规律，检测并分析一天内六个时间点(6:00、9:00、12:00、15:00、18:00、21:00)报春石斛花的香气成分。各时间点占总香气释放量排序为：6时(35.26%)>9时(22.78%)>12时(19.35%)>21时(7.86%)>15时(7.59%)>18时(7.17%)。共检测出202种香气物质，包括酯类、烯萜类、醇类、醛类、烷类等，其中6:00有93种，9:00有87种，12:00有68种，15:00有61种，18:00有63种，21:00有65种。各个时间点共有的化合物21种。报春石斛花香气释放量6:00最高，18:00最低，主要香气成分是醋酸辛酯。

关键词: 报春石斛, 香气, 日变化, 气相色谱-质谱联用