Abstract: In this paper, eight species of Dendrobium nobile were crossed and 13 hybrid combinations were formulated. Their F1 generation seeds were used as test material to investigate the effects of different capsule maturity and medium on the germination. And then the hormone concentrations of protocorm induction were optimized. The results showed that capsule maturity of 180-200 days was optimal for non-symbiotic germination of Dendrobium nobile hybrid seeds, as the short sprouting time and good sprouting at this stage. The germination rates of hybrid seeds in descending order on 5 different culture media are as follows: MS, N6, 1/2MS, KC, B5. MS medium was the most suitable medium with a germination rate up to 90.27%. After seed germination, the differentiation of protocorms were good, and seedlings were formed quickly. The best medium for the differentiation of protocorm of Dendrobium nobile hybrid seeds was MS+ NAA 0.2 mg·L^–1 + 6-BA 1.0 mg·L^–1, and the culture was transferred at 35-45 days.

Key words: Dendrobium nobile, hybrid seeds, non-symbiotic germination

摘要： 对8个春石斛品种进行杂交，配制杂交组合13个，以杂交F1代种子为试材，研究不同蒴果成熟度、培养基对春石斛杂交种子萌发的影响，并对后续原球茎生长分化条件进行优化。结果表明，蒴果成熟180～200 d为春石斛杂交种子非共生萌发最佳状态，此阶段萌发时间短，萌发效果好；5种培养基的春石斛杂交种子萌发率从高到低依次为MS、N6、1/2MS、KC、B5，MS培养基为最适培养基，其萌发率可达90.27%，种子萌发后原球茎分化效果好，成苗快；春石斛杂交种子原球茎分化最佳培养基为MS + NAA 0.2 mg·L^–1 + 6-BA 1.0 mg·L^–1，培养35～45 d时转接，是春石斛杂交种子原球茎分化的最佳时期。

关键词: 春石斛, 杂交种子, 非共生萌发