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Study on H2-oxidizing System in Bacteroid from Rhizobia arachis
Xu Liangshu, Zhang Fengzhang, Long Minnan, Huang Nanzhong
1994, 23(02):
1-7.
An investigation was conducted to identify electron transport carriers that participated the oxidation of H2 in bacteroids of Rhizobium arachis.H2-reduced mi-nus O2-oxidized absorption difference spectra in bacteroids showed peaks at 424,500,520,550,560,585 and 595 nm,indicating the involvement of c-,b-,a and o-types of cytochromes,The absorption peaks at 520,550,560 nm were diminished a lot in the pres-ence of CN- This showed that some of c- and b-type cytochromes were CN- sensitive and therefore might play a role as terminal oxidases in H2 metabolism. H2-uptake was in-hibited by antimycin A,HONO,CN-,N3- and DBMIB,not by rotenose,Our resultsindicate that ubiquinone and some types of cytochromes are involved in the oxyhydro-gen reaction which is a complex,branched transport system.
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