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10 June 1995, Volume 24 Issue 02 Previous Issue    Next Issue

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Diversity of Plasmid Profiles of Rhizobia Isolated from Eight Tropical and Subtropical Legumes Liu Hongzhou, Zou Xiaolu, Huang Weinan 1995, 24(02): 1-5.  Abstract ( )   PDF (274KB) ( )   Nine Rhizobium strains,isolated from eight species of tropical and sub-tropical legumes,were examined for plasmid content.Each of the fast-growing strain tested contained 1~6 plasmids wjth molecular weight from 32 kb to 870 kb.At least one of these plasmids was small (molecular weight was less than 100 kb),and three strainscontained only one small plasmid (molecular weight was less than 50kb).The plasmid profiles of Rhizobia from each species of the tropical and subtropical legumes were di-verse. Related Articles | Metrics

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Morphological Differentiation Period of Flower Buds on Prunus mume Varieties of Various Maturity Times Zhou Birong, Gan Liansheng, Qiu Jishui, Liang Jun, Lin Zhixiong 1995, 24(02): 6-9.  Abstract ( )   PDF (231KB) ( )   The growing points of the buds of mume (Prunus mume) varieties of various maturity times were observed under a scanning electron microscope to study the differentiation periods of their flower buds. The clear and intact pictures show that the flower buds of mume growing in Liannan county,Guangdong province begin to differentiate in the second and last ten days of July. Among them the mature period of the early variety (white powder mume) is comparatively short.It takes 3 months for flower buds to differentiate. The differentiation completes in the second and last ten days of October.,The mature periods of the medium and late varieties such as the large mume with tender twigs and May mume are longer.The differentiation continues until the second and last ten days of Deceniber,lasting 5 months. Related Articles | Metrics

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Methods for Immobilization of Pectinase Liu Xinmin, Lin Yaohui, Chen Yiliang, Shen Yufen 1995, 24(02): 10-15.  Abstract ( )   PDF (349KB) ( )   Sodium alginate,diacetate fibre and gelatin were used as carriers respec-tively for immobilization of pectinase.A comparison was made among the preparing con-ditions,i.e. the dosage of carrier,the concentration of cross linking agent,the dosage of pectinase and pH. The results showed that the recovery ratio of pectinase activity was up to 67.74% when the immobilized pectinase was prepared with 15% gelatin as carri-er,5% glutaric acid as cross linking agent and 5% pectinase at pH 3.5,and the enzyme activity retained more 80% after recovering 10 times repeatedly.This suggests that gela-tinization is a better method for immobilization of pectinase. Related Articles | Metrics

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Stepwise Discriminant Analysis of Cunninghamia lanceolata (Lamb.) Hook,Taiwania cryptomerioides Hayata and C. lanceolata (Lamb.) Hook.var. konisii (Hay.) Fujita Liang Yichi, Qiu Kuidong 1995, 24(02): 16-21.  Abstract ( )   PDF (331KB) ( )   Stepwise discriminant analysis was made from the data obtained from 9 traits of leaves (i.e. leaf length,leaf width,leaf thickness,width and thickness of vascular bundle,thickness of palisade tissue and mechanical tissue,thickness of epidermis and number of stomata),originating from 22 provenances of Cunninghamia lanceolata (Lamb.) Hook,Taiwania cryptomerioides Hayata and C. lanceolata (Lamb.) Hook.var.konisii (Hay.)Fujita. The optimized discriminant formula was developed by selecting thickness of palisade tissue (X6),leaf length (X1) and leaf thickness (X3) as three important factors,i.e. F1(Taiwania cryptomerioides Hayata)=-235.8714+17.3104X6+26.3587X1+5.9046X3 F2(Cunninghamia lanceolata (Lamb.) Hook)=-564.9259+78.4254X6+93.1923X1+0.6295X3 F3(C. lanceolata (Lamb.) Hook.var. konisii (Hay.) Fujita)=-349.4205+64.1926X6+62. 6479X1+0.8969X3 and can be used to differentiate quantitively these three species, aiming at probing into the application of stepwise discriminant analysis to the classfication of tree species. Related Articles | Metrics

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Nitrogenase and Hydrogenase Activity in the Nodules of Dalbergia balansae Xiaolu, Cai Keqiang, Huang Weinan 1995, 24(02): 22-25.  Abstract ( )   PDF (265KB) ( )   Nitrogenase and hydrogenase activities were determined and the relation-ship between them was investigated in the nodules of Dalbergia balansae under different environmental conditions,Hydrogen uptake activity was found in the nodules of D. balansae. In the presence of exogenous hydrogen,nitrogenase activity in nodules increased markedly,suggesting that hydrogenase played an important role in increasing the efficiency of nitrogen-fixation in symbiotic system. Low level of nitrate did not influence the nitrogenase and hydrogenase activities in the nodules,however,ammonium showed a negative effect on the expression of both enzyme activities. The optimum temperature for both nitrogenase and hydrogenase activities was about 25℃. Minor soil water deficit did not affect the acetylene-reducing activity,but serious soil drought markedly reduced the activities of nitrogen-fixation and hydrogen uptake. Shading reduced both nitrogenase and hydrogenase activities of nodules. Related Articles | Metrics

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Biological Characteristics and Cultural Practices of Nervilia plicata Chen Zhongren, Zhang Yongtian 1995, 24(02): 26-30.  Abstract ( )   PDF (293KB) ( )   The biological characteristics and cultural practices of the medicinal plant Nervilia plicata were studied.One young leaf emerges from each of the terminals of underground tubers or elongate rhizomes once a year.The underground parts of the plants develop with symbiotic fungi.Their young leaves arise in spring and die in late autumn,so the life period lasts about 169 days, Flower buds appear in mid May and fruits mature in mid June. It may take 30 days for flowering.N. plicata usually grows underthe bush-wood and it is adaptive to acid moist red soil or sandy loam with pH 5.5~6.5, loose and well drained.It is important to prevent pests and diseases during cultiva-tlon. Related Articles | Metrics

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Determination of Ginsenoide Rbl in Gynostemma pentapyllum by Thin Layer Chromatographic Scanning Liu Hongzhou, Tong Qingxuan, Wu Liangcai 1995, 24(02): 31-34.  Abstract ( )   PDF (226KB) ( )   Ginsenoside Rb1 was determined in MethOH extraction of Gynostemma pentaphyllum by HPTLC-scanning.Standard ginsenoside Rb1 was applied as the refer-ence curve,the linear range was 0.025~0.50 mg/ml,the correlation coefficient was 0.9993.The content of ginsenoside Rb1 was about 0.20%,which was higher in leaves than in stems,Ginsenoside Rb1 prepared by TLC and standard ginsenoside Rb1 were ap-plied at the same silica G plate and were hydrolysed with hydrogen chloride vapor for 25 min at 60℃. The combined sugars of both saponins were glucose,corresponding to that of the reference. Related Articles | Metrics

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Population Structure of Plant Nematodes in the Rhizosphere of Fruit Trees Zhang Shaosheng, Cai Xueqing 1995, 24(02): 35-40.  Abstract ( )   PDF (351KB) ( )   Ten genera of plant nematodes,i.e. Aphelenchoides,Aphelenchus,Tylenchus,Paratylenchus,Pratylenchus, Helicotylenchus,Rotylenchus,Hemicriconemoides,Cricomemoides,Trichodorus were identified from the roots and rhizosphere soil of longan (Euphoria longana), Chinese olive (Canarium album),loquat (Eriobotrya japonica) and grape (Vitis spp.) trees, which were growing at a given location on Jiuhua Farm, Putian city,Fujian province. The investigative results show that the kinds of fruit tree influence the genera or species and the population structure of plant nematodes. There are sixgen-era of plant nematodes on longan, the nematode populations consist of 2~4 genera,the dominant nematode is Trichodorus. There are 7 genera of nematodes on Chinese olive,the populations consist of 1~7 genera,the dominant nematode is Paratylenchus. Thereare 6 genera of plant nematodes on loquat,the populations consist of 2~5 genera,the dominant nematode is Paratylenchus.There are 5 genera of plant nematodes on grape,the populations consist of 2~5 genera,Helicotylenchus widely distributes but the popu-lation of Pratylenchits is of highest density. Related Articles | Metrics

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Introduction and Cultivation of Dipterocarpus tuberoulatus YangQing, Xiao Laiyun, Pu Zhenghe 1995, 24(02): 41-45.  Abstract ( )   PDF (330KB) ( )   Diptercarpits tuberculatus was introd uced from Thailand into Xishuang-banna,Yunnan Province in 1980.Thirteen-year cultivation showed that the plants grew fastar than in some areas of their native habitat.The flowering and fruiting of the plants were normal.A group of seedlings were grown by seeds. The determination of fallen leaf amount and undersoil nutrient showed that the plant might serve as a species for conservation of soil and water as well as amelioration of soil.The feasibility of large scale development of D. tuberculatus in tropical area of China is also evaluated in regard to the utilization value of the species, environmental requirements of afforestation and the conditions of its external cultivation and economic benefit as well. Related Articles | Metrics

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The Use of Common Palmsin Landscape Gardening Chen Hengbin, Zhou Xinyue 1995, 24(02): 46-50.  Abstract ( )   PDF (322KB) ( )   The palms have beautiful shape,they are used in landscape gardening as scenic trees of paths,parks, factories and courtyards as well as potted plants. The ways of using palms in the future are discussed. Related Articles | Metrics

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Revision of Heteropanax chinensis (Dunn) Li Wu Darong, ji Xiangmin 1995, 24(02): 51-53.  Abstract ( )   PDF (196KB) ( )   According to the characteristics of morphology and distribution of Heteropanax chinensis (Dunn) Li and H. brevipedicellatus Li,the two species should be revised as one species. Related Articles | Metrics

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Postharvest Physiology of Carambola Fruits Li Jinyu, Huang Weinan 1995, 24(02): 54-58.  Abstract ( )   PDF (318KB) ( )   The main nutritients,respiration,ethylene production and main physiological diseases of carambola fruits are outlined.The effects of various storage conditions or treatments on postharvest physiological and biochemical changes in the fruits are also summarized. Related Articles | Metrics

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Sesbania rostrata and Other Stem-modulated Plants Chen Jinbiao, Hong Li, Lou Wujiand, Fan Qingsheng 1995, 24(02): 59-64.  Abstract ( )   PDF (464KB) ( )   In symbiosis with Rhizobium sp,most leguminous plants form nitrogen-fixing nodules on their roots,Recent progress has been made in the research of stem-nodulated plants whose dual nodulation confers upon them an unusually high nitrogenfixing potential.They mainly belong to three genera:Sesbania,Aeschynomene and Neptunia.The object of this paper is to review the available informaton on stem nodule and its application,with special emphasis on Sesbania rostrata. Related Articles | Metrics