Subtropical Plant Science ›› 2024, Vol. 53 ›› Issue (5): 399-407.DOI: 10.3969/j.issn.1009-7791.2024.05.002

• Research articles • Previous Articles     Next Articles

Determination of 10 Phenylethanol Glycosides in Cistanche deserticola from Different Origins and 2 Species of Host Plants Based on UPLC-QQQ-MS/MS

ZHAO Fa-fa, GUO Ye-hong*, GAO Pei-wen, CHEN Jin-hui, ZHANG Wen-yao   

  1. (College of Agronomy, Gansu Agricultural University / State Key Laboratory of Aridland Crop Science, Lanzhou 730070, Gansu China)
  • Received:2024-04-16 Accepted:2024-06-07 Online:2024-10-31 Published:2025-01-25
  • Contact: GUO Ye-hong

基于UPLC-QQQ-MS/MS测定不同产地2种寄主肉苁蓉10种苯乙醇苷成分

赵法法,郭晔红*,高培文,陈锦辉,张文瑶   

  1. (甘肃农业大学农学院 / 省部共建干旱生境作物学国家重点实验室,甘肃 兰州 730070)
  • 通讯作者: 郭晔红
  • 基金资助:
    国家自然科学基金(31860349);甘肃省科技计划(18YFlNA072)

Abstract: Ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QQQ- MS/MS) was used for the simultaneous determination of 10 phenylethanol glycosides in Cistanche deserticola of 2 host species from different origins. The results showed that the linearity of the 10 components to be measured was good in their respective ranges (R2>0.9989), and the average recoveries of the controls were in the range from 94.61% to 102.71%, with the RSD values less than 3.39%, which indicated that the results of the established method were accurate. The total amount of phenylethanol glycosides of C. deserticola from Inner Mongolia was significantly higher than those of C. deserticola from Xinjiang and Gansu. The total amount of phenylethanol glycosides of Atriplex canescens – C. deserticola was in the order of Inner Mongolia>Xinjiang>Gansu, while the total amount of phenylethanol glycosides of Haloxylon ammodendron C. deserticola was in the order of Inner Mongolia>Gansu>Xinjiang. The total amount of phenylethanol glycosides of A. canescens – C. deserticola from the 3 origins was higher than those of H. ammodendron – C. deserticola. It provided a more convenient assay for the determination of phenylethanol glycosides among C. deserticola of different origins and different hosts, and also provided scientific ideas for the quality evaluation and host selection of C. deserticola.

Key words: Cistanche deserticola, UPLC-QQQ-MS/MS, producing area, host plants, phenylethanol glycosides

摘要: 采用超高效液相色谱-三重四杆串联质谱法(UPLC-QQQ-MS/MS)测定不同产地2 种寄主肉苁蓉Cistanche deserticola中 10 种苯乙醇苷类成分含量。结果表明,10 种待测成分分别在各自范围内线性关系良好(R2>0.9989),各对照品的平均回收率为94.61%~102.71%,RSD值小于3.39%,所建立的测定方法结果准确。内蒙古肉苁蓉苯乙醇苷总量显著高于产自新疆和甘肃的肉苁蓉,四翅滨藜Atriplex canescens–肉苁蓉苯乙醇苷总量由高到低依次为内蒙古>新疆>甘肃,梭梭Haloxylon ammodendron–肉苁蓉苯乙醇苷总量则依次为内蒙古>甘肃>新疆。3个产地的肉苁蓉苯乙醇苷总量均以四翅滨藜–肉苁蓉高于梭梭–肉苁蓉。研究结果为测定肉苁蓉苯乙醇苷类成分含量提供更便捷的检测方法,也为肉苁蓉质量评价与寄主选择提供依据。

关键词: 肉苁蓉, 超高效液相色谱-三重四杆串联质谱法, 产地, 寄主植物, 苯乙醇苷类

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