Subtropical Plant Science ›› 2015, Vol. 44 ›› Issue (01): 13-17.DOI: 10.3969/j.issn.1009-7791.2015.01.003
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LI Bin,LI Gui,CHEN Gong-xi,LI Hui,LIANG Jun-peng,CHEN Ya
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李斌,李贵,陈功锡,李辉,梁俊鹏,陈雅
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湖南省教育厅重点项目(09A073)
Abstract: Polysaccharides (PS) from the fruits of Campnumoea lancifolia were extracted and purified by ethanol precipitation, ethyl acetate and sevage reagent with ultrasonic assistance, and the content was determined by the phenol-sulfuric acid method. The components of the polysaccharides were identified by gas chromatography-mass spectrometry (GC-MS). The results showed that the average content of polysaccharide in the fruits of C. lancifolia reached up to 45.80%, and main monosaccharide composition and relative contents were arabia sugar (5.00%), xylose (9.65%), mannose (11.55%), fructose (30.20%), galactose (33.85%).
Key words: Campnumoea lancifolia, polysaccharide, ultrasonic extraction, monosaccharide composition, GC-MS, Campnumoea lancifolia, polysaccharide, ultrasonic extraction, monosaccharide composition, GC-MS
摘要: 用超声波法提取红果参果实的多糖,并分别以乙醇提取,乙酸乙酯、sevage试剂等进行纯化,以苯酚-硫酸法测定多糖含量,以GC-MS测定其单糖组分。结果表明,红果参果实中多糖含量高达45.80%,其主要单糖组分及百分比为阿拉伯糖(5.00%)、木糖(9.65%)、甘露糖(11.55%)、果糖(30.20%)、半乳糖(33.85%)。
关键词: 红果参, 多糖, 超声提取, 单糖组成, GC-MS, 红果参, 多糖, 超声提取, 单糖组成, GC-MS, 红果参, 多糖, 超声提取, 单糖组成, GC-MS
LI Bin,LI Gui,CHEN Gong-xi,LI Hui,LIANG Jun-peng,CHEN Ya. Extraction and Composition of Polysaccharide from the Fruits of Campnumoea lancifolia[J]. Subtropical Plant Science, 2015, 44(01): 13-17.
李斌,李贵,陈功锡,李辉,梁俊鹏,陈雅. 红果参果实多糖的提取及其单糖组分研究[J]. 亚热带植物科学, 2015, 44(01): 13-17.
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