Abstract: In order to explore the effects of light on the yield and accumulation of volatile oil components of Artemisia argyi, the tissue culture seedlings of A. argyi were treated with different light quality and intensity. The results showed that the biomass and volatile oil components of A. argyi leaves were obviously changed under different light treatments. The biomass increased obviously under high light intensity and blue light treatments, and the plant height were elongation under red and blue light treatments. The contents of volatile oil, contained 1,8-cineole and β-Terpineobe, were significantly increased under high light intensity, red light and blue light treatment. High light intensity also promoted borneol accumulation. Interestingly, terpenes, isoborneol and eucalyptol were detected under blue light treatment, which were not detected in other light treatment. Moreover, the gene expression levels of photoreceptors and light-responsive transcription factors under different light treatments were analyzed. Its expression level was significantly increased under high light intensity treatment. The correlation analysis between the expression of photoreceptor genes and the biomass and the main volatile oil of A. argyi were carried out. And there was a significant correlation between the expression levels of photoreceptor-related genes and the biomass and volatile oil components. The results showed that the biomass and volatile oil content of A. argyi were regulation under different light treatments, which maybe through the regulation of gene expression of photoreceptors. In conclusion, the biomass and metabolites of A. argyi were increased under high light intensity and blue light treatment, which could give a foundation of cultivation in A. argyi. This study found that the biomass, volatile oil accumulation and photoreceptor genes were changed under different light treatments.

Key words: Artemisia argyi, volatile oil, light, gene expression

摘要： 为探究光照对艾(Artemisia argyi)的产量及主要挥发油成分积累的影响，以蕲艾组培苗为试材，采用高光强、红光、蓝光等进行不同光强光质处理实验。结果表明，高光强及蓝光处理可促进艾生物量积累，红光和蓝光处理可促进植株伸长。高光强、红光及蓝光均可提高艾叶挥发油中1,8-桉油素、β-萜品醇含量。高光强还能促进龙脑积累，而蓝光处理下可检测到较多在其他光照条件下未检测到的化合物，如萜烯和蓝桉醇等物质。对不同光强光质处理下光受体及光响应转录因子基因表达量进行分析，在高光强处理下，所有光受体的表达量均显著升高；而响应不同光质的对应光受体基因表达量增加。光受体基因表达与艾生物量及主要挥发油的相关性分析表明，光受体基因表达对艾草生物量的影响起重要作用，且不同光处理调控可通过光受体响应并级联调控相关基因表达，调控艾叶生长及挥发油的合成。高光强和蓝光处理下，艾草的生物量及有效挥发油代谢物增加，可在艾草栽培中加以应用。

关键词: 艾叶, 挥发油, 光, 基因表达