Evaluation of Cold Resistance of 9 Clones of Acacia mangium × A. auriculiformis

doi:10.3969/j.issn.1009-7791.2025.01.006

Subtropical Plant Science ›› 2025, Vol. 54 ›› Issue (1): 45-53.DOI: 10.3969/j.issn.1009-7791.2025.01.006

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Evaluation of Cold Resistance of 9 Clones of Acacia mangium × A. auriculiformis

LIAO Fang-yan¹, CHEN Bo-zhen^2,3, WANG Li-han^2,3, WANG Yu-xin^2,4, LI Na², ZHU Bao-zhu², ZHANG Wei-hua^2*

(1. Guangdong Forestry Affairs Center, Guangzhou 510173, Guangdong China; 2. Guangdong Academy of Forestry, Guangzhou 510520, Guangdong China; 3. College of Forestry, Hebei Agricultural University, Baoding 071001, Hebei China; 4. College of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang China)

Received:2024-09-06 Accepted:2024-12-17 Online:2025-02-28 Published:2025-05-22
Contact: ZHANG Wei-hua

马大相思9个无性系抗寒性评价

廖仿炎¹，陈泊臻^2,3，王力涵^2,3，王雨欣^2,4，李娜²，朱报著²，张卫华^2*

(1. 广东省林业事务中心，广东广州 510173；2. 广东省林业科学研究院，广东广州 510520；3. 河北农业大学林学院，河北保定 071001；4. 东北林业大学林学院，黑龙江哈尔滨 150040)

通讯作者: 张卫华
基金资助:
国家自然科学基金(32271918)

Abstract

Abstract: The seedlings of 9 clones of Acacia mangium × A. auriculiformis were used as experimental materials, and the cold environment was simulated by a large low-temperature artificial climate chamber. Physiological indexes such as relative electrical conductivity (REC), soluble protein (SP), free proline (Pro) and malondialdehyde (MDA) were determined, and the response of physiological indexes, cold resistance were analyzed under low temperature conditions, and the cold resistance of 9 clones of A. mangium × A. auriculiformis were evaluated by membership function method comprehensively. Results showed that the relative electrical conductivity and soluble protein content of leaves of different clones increased gradually, the free proline content and SOD activity increased first and then decreased, and the MDA content increased first and then decreased and then increased under low temperature stress. According to the average membership function value, the rank of the cold resistance of 9 clones of A. mangium×A. auriculiformis is No. 8 > No. 7 > No. 6 > No. 2 > No. 1 > No. 9 > No. 3 > No. 4 > No. 5. The No. 8 has the strongest cold resistance, and it can be used as a high quality cold resistance material for hybrid breeding and cultivation.

Key words: Acacia mangium × A. auriculiformis, clones, physiological index, cold resistance, membership function method

摘要： 以马大相思Acacia mangium × A. auriculiformis 9个无性系幼苗为试材，利用大型低温人工气候室模拟阶段降温寒冷环境，测定幼苗叶片相对电导率(REC)、可溶性蛋白(SP)、游离脯氨酸(Pro)、丙二醛(MDA)等生理指标，分析低温条件下马大相思生理指标的响应及抗寒性，并利用隶属函数法对马大相思9个无性系抗寒能力进行综合评价。结果表明，低温胁迫下，马大相思不同无性系间叶片相对电导率和可溶性蛋白含量均逐渐增加，Pro含量和SOD酶活性表现为先升后降趋势，MDA含量呈现先升高后降低再升高的规律。根据平均隶属函数值得到马大相思9个无性系抗寒性强弱综合排序依次为：8号>7号>6号>2号>1号>9号>3号>4号>5号。8号无性系抗寒能力最强，可作为马大相思抗寒杂交育种和栽培的优质材料。

关键词: 马大相思, 无性系, 生理指标, 抗寒性, 隶属函数法

CLC Number:

S722

LIAO Fang-yan, CHEN Bo-zhen, WANG Li-han, WANG Yu-xin, LI Na, ZHU Bao-zhu, ZHANG Wei-hua. Evaluation of Cold Resistance of 9 Clones of Acacia mangium × A. auriculiformis[J]. Subtropical Plant Science, 2025, 54(1): 45-53.

廖仿炎, 陈泊臻, 王力涵, 王雨欣, 李娜, 朱报著, 张卫华. 马大相思9个无性系抗寒性评价[J]. 亚热带植物科学, 2025, 54(1): 45-53.

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Evaluation of Cold Resistance of 9 Clones of Acacia mangium × A. auriculiformis

马大相思9个无性系抗寒性评价

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