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Subtropical Plant Science

Subtropical Plant Science ›› 2005, Vol. 34 ›› Issue (01): 64-64.

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Primary Study on Tissue Culture of High Stalk Japanese Broccoli

LAI Zheng-feng, LIN Jia-geng, LI Hua-dong, ZHENG Jia-xie   

  1. Fujian-Taiwan Horticulture Research Center, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian China
  • Received:2004-11-01 Online:2005-03-10 Published:2005-03-10

日本高杆青花菜组织培养初探

赖正锋,林加耕,李华东,郑加协   

  1. 福建省农科院 闽台园艺研究中心, 福建 漳州 363005
  • 作者简介:赖正锋:(1975-),男,福建三明人,研究实习员,学士,从事蔬菜开发研究工作。

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摘要： 以日本高杆青花菜(スティックセニョール)茎段及带叶腋芽为外殖体,接种于MS+BA 3.0mg/L+NAA 0.5mg/L培养基上培养20d,腋芽萌发生长成2~3cm高的芽苗,而茎段则膨大后从切口生长出淡绿色愈伤组织,并分化出大量丛生芽。将幼苗转接到MS+BA2.0mg/L+NAA0.2mg/L培养基上进行增殖培养,繁殖系数为4~6,待芽长2~3cm时再分切成单株于1/2MS+NAA0.2mg/L+IBA0.1mg/L培养基上进行生根培养,25d后,生根率可达86%。在温度20~25℃条件下,移栽成活率82%。

关键词: 青花菜, 茎段, 腋芽, 组织培养

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