Acquisition of Friable Calli from Leaves and Establishment of the Cell Suspension Culture of Tobacco

Subtropical Plant Science ›› 2005, Vol. 34 ›› Issue (01): 63-63.

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Acquisition of Friable Calli from Leaves and Establishment of the Cell Suspension Culture of Tobacco

ZHENG Yuan, XIAO Ya-ping

College of Life Science, Shanxi Normal University, Xi'an 710062, Shanxi China

Received:2004-11-08 Online:2005-03-10 Published:2005-03-10

烟草脆散型愈伤组织获取及其细胞悬浮培养

郑媛,肖娅苹

陕西师范大学生命科学学院, 陕西西安 710062

作者简介:郑媛(1979-),女,陕西西安人,硕士研究生,从事植物组织培养研究。

Abstract

摘要： 以烟草叶片为外植体,以2/3MS+BA 0.5mg/L+NAA 0.1mg/L为愈伤组织诱导培养基,继代培养3次后,转入添加3332.48mg/L Ca(NO₃)₂的该培养基上继代,可获得脆散型愈伤组织,并由此建立细胞悬浮培养系统。

关键词: 烟草叶片, 脆散型愈伤组织, 细胞悬浮培养, 钙

CLC Number:

Q943.1

ZHENG Yuan, XIAO Ya-ping. Acquisition of Friable Calli from Leaves and Establishment of the Cell Suspension Culture of Tobacco[J]. Subtropical Plant Science, 2005, 34(01): 63-63.

郑媛,肖娅苹. 烟草脆散型愈伤组织获取及其细胞悬浮培养[J]. 亚热带植物科学, 2005, 34(01): 63-63.

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Acquisition of Friable Calli from Leaves and Establishment of the Cell Suspension Culture of Tobacco

烟草脆散型愈伤组织获取及其细胞悬浮培养

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