Abstract: The effects of different type biochars such as fruit shell biochar, coconut shell biochar and straw biochar on photosynthetic characteristics and gypenosides content of Gynostemma pentaphyllum were researched. At the same time, the correlation of chlorophyll contents and chlorophyll fluorescence parameters were analyzed. The results showed that chlorophyll contents and chlorophyll fluorescence parameters of G. pentaphyllum leaves were enhanced by treatment of biochars. The chlorophyll contents, real time Yield and qP were highest at treatment of straw biochar while qN and NPQ was lowest. The patterns of the light response curve and light inductive curve were consistent at different treatments. The Yield and ETR at treatment of straw biochar was highest in light response curve and light inductive curve. Correlation analysis results showed that they had a very significant positive correlation among chlorophyll a, chlorophyll b and total chlorophyll content. Yield had a positive correlation with qP, and a very significant negative correlation with qN and NPQ. qP had a very significant negative correlation with qN, and a negative correlation with NPQ. qN had a very significant positive correlation with NPQ. Chlorophyll a and total chlorophyll content had a very significant positive correlation with Yield, and a significant positive correlation with qP, while it had a very significant negative correlation with qN. NPQ had a significant negative correlation with chlorophyll a, while it had a negative correlation with total chlorophyll content. Chlorophyll b had a significant positive correlation with Yield and qP, and a very significant negative correlation with qN, which had a negative correlation with NPQ. Gypenosides content enhanced at different treatment of biochars, the gypenosides content of straw biochar was 1.54, 1.15 and 1.35 times of that of control, fruit shell biochar and coconut shell biochar respectively.

Key words: Gynostemma pentaphyllum; biochar; photosynthetic characteristics; gypenosides

摘要： 以绞股蓝Gynostemma pentaphyllum为试材，研究果壳炭、椰壳炭和秸秆炭等类型生物炭对绞股蓝光合特性和总皂苷含量的影响，并对叶绿素含量和叶绿素荧光特性参数进行相关性分析。结果表明，栽培基质添加生物炭可有效提高绞股蓝叶绿素含量和叶绿素荧光参数，且以添加秸秆炭的叶绿素含量、实时PSII有效光化学量子产量 (Yield)和光化学淬灭系数(qP)最高，非光化学淬灭系数(qN和NPQ)最低。不同类型生物炭处理的绞股蓝叶片光响应曲线和光诱导曲线的变化规律一致，以添加秸秆炭的Yield和电子传递速率(ETR)最高。相关性分析表明，叶绿素a、叶绿素b、总叶绿素含量之间极显著正相关。实时荧光参数Yield与qP正相关，与qN、NPQ极显著负相关；qP与qN极显著负相关，与NPQ负相关；qN与NPQ极显著正相关。叶绿素a含量和总叶绿素含量与Yield极显著正相关，与qP显著正相关，与qN极显著负相关。叶绿素a含量与NPQ显著负相关，总叶绿素含量与NPQ负相关。叶绿素b含量与Yield、qP显著正相关，与qN极显著负相关，与NPQ负相关。添加生物炭提高绞股蓝总皂苷含量，添加秸秆炭的皂苷含量分别为对照、果壳炭和椰壳炭的1.54、1.15和1.35倍。

关键词: 绞股蓝；生物炭；光合特性；绞股蓝总皂苷