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Subtropical Plant Science

Subtropical Plant Science ›› 2006, Vol. 35 ›› Issue (02): 63-63,65.

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Tissue Culture and Rapid Propagation of Trapa acornis

ZHAN Li-juan1, LAI Qi-xian1, ZHU Zhu-jun1, MAO Bi-zeng2, GE Zhi-ping3   

  1. 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang China;
    2. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang China;
    3. Seed Station, Bureau of Agricultural Economy and Technology of Jiaxing, Jiaxing 314000, Zhejiang China
  • Received:2005-12-30 Online:2006-06-10 Published:2006-06-10

南湖菱的组织培养与快速繁殖

詹丽娟1,赖齐贤1,朱祝军1,毛碧增2,葛志平3   

  1. 1. 浙江大学 农业与生物技术学院 园艺系, 浙江 杭州 310029;
    2. 浙江大学 生物技术研究所, 浙江 杭州 310029;
    3. 嘉兴市农业经济技术局 种子站, 浙江 嘉兴 314000
  • 作者简介:詹丽娟 (1978-),女,河南信阳人,硕士研究生,从事蔬菜生理生化研究。

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摘要： 以南湖菱(Trapa acornis Nakano)种子为外植体,在1/2 MS和Nitsch液体培养基上均可萌发成无菌苗;无菌苗带芽茎段在Nitsch+6-BA 0.5 mg/L+NAA 0.2 mg/L+0.5%活性炭的增殖培养基上,增殖倍数可达3.4;壮苗后转入1/2 MS+NAA 0.2 mg/L+1.5%活性炭的生根培养基,生根率80%以上。

关键词: 南湖菱, 种子, 无菌苗, 组织培养

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