Top Read

Published in last 1 year |

In last 2 years |

In last 3 years |

All

Please wait a minute...


For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails

Select

New Records of Five Ferns from Guangdong Province GUO Xiao-feng, YAN Yue-hong, GU Yu-feng, WEI Xue-ying, ZHENG Xi-long, SHU Jiang-ping Subtropical Plant Science    2023, 52 (2): 167-170.   DOI: 10.3969/j.issn.1009-7791.2023.02.012 Abstract （222）      PDF（pc） （3431KB）（368）       Knowledge map    Save Five newly recorded species belonging to 4 families and 5 genera of ferns from Guangdong province were reported, they are Adiantum menglianense Y. Y. Qian, Leptochilus × shintenensis (Hayata) X. C. Zhang & Noot., Oreogrammitis adspersa (Blume) Parris, Hymenasplenium pseudobscurum Viane, Diplopterygium simulans (Ching) Ching ex X. C. Zhang. The voucher specimens are deposited in the Herbarium of Shenzhen Orchid Conservation and Research Centre (NOCC). Reference | Related Articles | Metrics | Comments（0）

Select

Additions to the Flora of Hong Kong, China ZHU Hui-ling, LIU Jin-gang, WONG Chuk-kwan, Craig WILLIAMS, HANG King-yeung, ZHANG Jin-long, Stephan W. GALE Subtropical Plant Science    2023, 52 (1): 70-77.   DOI: 10.3969/j.issn.1009-7791.2023.01.011 Abstract （192）      PDF（pc） （7599KB）（119）       Knowledge map    Save Six native and two naturalised vascular plant species are newly added to the flora of Hong Kong SAR, China. The newly recorded native species are Aphanamixis polystachya (Wall.) R. Parker, Oplismenus undulatifolius (Ard.) Roemer & Schuit., Sageretia ellipsoidea Yi Yang, H. Sun & H. Peng, Sageretia hamosa (Wall.) Brongn., Trachelospermum brevistylum Hand.-Mazz. and Zingiber hainanense Y. S. Ye, L. Bai & N. H. Xia; and the two naturalised species are Alysicarpus ovalifolius (Schumach.) J. Léonard and Melothria pendula L. This is the first time the genera Aphanamixis Blume and Melothria L. have been recorded in the territory. Notes on distribution, habitat, phenology, taxonomy and key diagnostic features are provided. Reference | Related Articles | Metrics | Comments（0）

Select

Field Investigation and Analysis of a Rare Abnormal Fruit Occurrence in ‘Guiwei’ Lychee LIU Hong-feng, HUANG Chuan, CHEN Chun-fan, CHEN Feng-xiang, LU Mei-ying, CHEN Yan-yan, DING Feng, LU Rong-qing, LI Ye-qing, LI Fang-wen, PENG Hong-xiang Subtropical Plant Science    2023, 52 (2): 93-100.   DOI: 10.3969/j.issn.1009-7791.2023.02.002 Abstract （189）      PDF（pc） （651KB）（611）       Knowledge map    Save In response to the rare “brush tip” shaped abnormal fruit problem that occurred in a large number of ‘Guiwei’ lychee (Litchi chinensis) orchards in the Qinzhou production area of Guangxi in the spring of 2022, in April (flower withering), four ‘Guiwei’ lychee orchards in Lingshan County were selected for investigation analysis and one control orchard no abnormal fruit. The aim is to further analyze and explore the process and reasons for the formation of this abnormal fruit, and provide reference for the safe use of exogenous hormones for promoting flowering and fruit preservation in lychee. The survey results indicated that the formation of abnormal fruit in lychee began from the elongation of the stigma during the differentiation of female flowers, and then evolved into a completely fleshless and empty shell shaped “brush tip” abnormal fruit. This “brush tip” shaped abnormal fruit was not easy to fall off and completely lost its commercial value; The rate of deformed fruit plants in the four orchards was 94.0%–100.0%, the rate of deformed fruit ears per plant was 20.0%–24.0%, and the rate of deformed fruit grains per ear was 60.9%–65.1%. There was no significant difference in the three indicators among the four lychee orchards. All four orchards used “Baoying” products containing phenylpeptidylamine acid during the shoot control, flower promotion, and fruit preservation period. Other control orchards that did not use phenylpeptidylamine acid to promote flower and fruit preservation did not have any abnormal fruit occurrence; It is preliminarily believed that ‘Guiwei’ lychee is sensitive to phenylpeptidylamine acid during flowering and fruiting under the climatic conditions of that year, which can cause abnormal elongation of the female flower stigma and affect pollination, fertilization, and fruit development, ultimately forming empty shell deformed fruits without edible value. Reference | Related Articles | Metrics | Comments（0）

Select

A Simple Method of Making Leaf Vein Specimens and Using Image J Software to Analyze the Characteristics of Leaf Vein QUAN Chang-bin, YAO Yu-xian, MO Guang-you Subtropical Plant Science    2022, 51 (5): 397-404.   DOI: 10.3969/j.issn.1009-7791.2022.05.011 Abstract （184）      PDF（pc） （643KB）（77）       Knowledge map    Save The leaves of 12 species of plants were treated with 5% potassium hydroxide boiling water bath, and the mesophyll and epidermis were separated by ultrasonic cleaning. The leaf vein was prepared by bleaching and dyeing. The specimens were imaged using digital camera and microscope, and the images were analyzed using the macro analysis steps of Image J software MINA tool. The density, branches density, junction density and end-point voxels density of leaf veins of 12 plants were measured, and the accuracy of the measurement results was verified by manual tracking. The results showed that the experimental method could quickly obtain clear and complete vein network structure, and use Image J software MINA tool macro analysis steps to analyze the vein. Compared with the traditional sodium hydroxide tissue transparency method, this experimental method had shorter processing time, complete and clear leaf vein network structure, and the leaf vein density could be accurately measured by Image J software. Reference | Related Articles | Metrics | Comments（0）

Select

Flora of Lycophytes and Ferns in Dawangling Nature Reserve, Guangxi LI Yue, YAO Zhi-yong, LING Cheng-li, FANG Chao-hui, WEI Yan-lin, WEI Wen-gang Subtropical Plant Science    2023, 52 (3): 258-264.   DOI: 10.3969/j.issn.1009-7791.2023.03.011 Abstract （180）      PDF（pc） （902KB）（163）       Knowledge map    Save On the basis of field vegetation survey, the species composition, floristic characteristics, endangered protection and economic value of lycophytes and ferns in Dawangling Nature Reserve, Guangxi were sorted out and analyzed according to PPG I classification system. The results showed that there were 28 families, 66 genera and 148 species of lycophytes and ferns in the reserve, the dominant families and genera were obvious and it was dominated by single species families and genera, with relatively ancient origin and relatively coherent evolutionary system; the analysis of floristic geographical components showed that the tropics and subtropics were dominant at the level of families, genus and species, and the R/T value of the genus was 8.8, showing the tropical element, and there was a significant difference with the adjacent areas. There were 8 species of lycophytes and ferns under second-class national protection and 4 species of vulnerable species under IUCN list. There were many species with high utilization value, which was mainly medicinal value. Reference | Related Articles | Metrics | Comments（0）

Select

Identification and Bioinformation Analysis of NRAMP Gene Family Based on Andrographis paniculata Genome FAN Pan-hui, WU Li-wei, SUN Wei, WANG Yu, GAO Wei, XING Jian-yong, SONG Jing-yuan, YAO Hui Subtropical Plant Science    2023, 52 (3): 175-182.   DOI: 10.3969/j.issn.1009-7791.2023.03.001 Abstract （161）      PDF（pc） （4165KB）（140）       Knowledge map    Save Natural resistance associated macrophage protein (NRAMP) is a major class of membrane transporters widely found in animals, plants and microorganisms, and play an important role in transporting and reusing heavy metal ions in plants. For exploring the biological characteristics of NRAMP gene family in Andrographis paniculata, seven NRAMP gene family members were screened and identified from the whole genome, named ApNRAMP1–ApNRAMP7, and their gene structures and physicochemical properties of the encoded proteins were analyzed. The results showed that the proteins encoded by ApNRAMPs genes ranged from 513 aa to 553 aa in length, and their molecular weight were between 56.00 kD and 60.54 kD. The subcellular localizations of all members were in the cell membrane. Phylogenetic analysis found that the NRAMP gene family was divided into two subfamilies. ApNRAMP3–ApNRAMP6 in Group Ⅰ contained 3 introns, while ApNRAMP1, ApNRAMP2, and ApNRAMP7 in Group Ⅱ contained 12 introns, and their encoded protein sequences all contained Motif1–8 sequences. Protein structure prediction analysis showed that the secondary structures of ApNRAMPs proteins were mainly composed of α-helix and random coil, and the three-dimensional structures were relatively similar. This study provides a reference for further elucidation of the molecular mechanism of ApNRAMPs genes involvement in cadmium stress response. Reference | Related Articles | Metrics | Comments（0）

Select

Subtropical Plant Science    2023, 52 (5): 0-.   Abstract （158）      PDF（pc） （1175KB）（139）       Knowledge map    Save Related Articles | Metrics | Comments（0）

Select

Neighbor Density and Habitat Heterogeneity Jointly Determined the Seedling Abundance in Gaoligong Mountains, Southwestern China WANG Li-ping, WU Jun-jie, CHAI Yong, YU Xiao-li, SUN Ji-wen, FENG Zhe, CHEN Feng-xian Subtropical Plant Science    2024, 53 (2): 89-99.   DOI: 10.3969/j.issn.1009-7791.2024.02.001 Abstract （156）      PDF（pc） （948KB）（130）       Knowledge map    Save To understand the crucial dependency factors of seedling abundance with a diameter at breast height (DBH) <1 cm in a mid-montane moist evergreen broad-leaved forest of 4 hm2 Gaoligong Mountains forest. The generalized linear mixed models were used to analyze whether neighbor density and habitat factors shaped seedling abundance at the community and species level, and whether the strength of them varied with temporal. The results showed that: (1) The effect of neighbor density and habitat factors on all seedling abundance were significant and gradually strengthen at the community level. The positive correlation of common species with heterospecific neighbor density increased significantly, while no correlation with environmental factors. The seedling abundance of rare species reflected non-significant with neighbor density and environmental factors. (2) At species level, the seedling abundance of Psychotria morindoides was only slightly and significantly negatively associated with elevation in 2022. From 2020 to 2023, the significant positive effects of biotic and habitat heterogeneity on seedling abundance of Myrsine semiserrata. The higher canopy openness, elevation, and soil total potassium and temperatures, the higher seedling abundance. However, these correlations were non-significant on the seedling abundance of Symplocos stellaris var. aenea in the four periods. This study indicated that the seedling abundance is driven by both neighbor density and habitat heterogeneity. The strength of neighbor densities and habitat factors differs greatly among community, species and temporals, which relative importance of variables can maintain the community diversity in the plot. Reference | Related Articles | Metrics | Comments（0）

Select

Effects of Aluminum on the Absorption of Nutrients and Enzyme Activity of Ectomycorrhizal Fungi LI Kuai-fen, LIU Hai-yan Subtropical Plant Science    2023, 52 (1): 9-17.   DOI: 10.3969/j.issn.1009-7791.2023.01.002 Abstract （152）      PDF（pc） （686KB）（58）       Knowledge map    Save Effects of treatment on biomass, pH, nutrient uptake, antioxidant enzyme activity, and MDA content of Suillus luteus (Sl), Suillus bovines (Sb) and Suillus placidus (Sp) were tested by liquid culture with different concentrations of Al3+ (0, 0.2, 0.4, 0.8, 1.2 and 1.6 mmol·L–1). The results showed that aluminum treatment significantly increased the biomass, P content, and Al content of the three strains, and decreased the pH in the culture medium. When the concentration of Al3+was 0.8 mmol·L–1, the biomass increase in the strain was the largest, and the aluminum content of the three strains treated with Al3+at various concentrations was Sp>Sb>Sl; The potassium content showed a trend of first increasing and then decreasing as the concentration of Al3+ increased. Low concentration of Al3+ (≤0.4 mmol·L–1) significantly promoted the accumulation of nitrogen in the Sl strain, and the treatment with 0.4 mmol·L–1 Al3+ increased by 42.65% compared to the treatment without aluminum. Aluminum treatment also significantly decreased the POD and SOD activities of the strain, and increased the MDA content of the strain. The biomass of the strain was significantly positively correlated with the N, P, and K content of the mycelium (P<0.01), with the highest correlation with P content (r=0.635). To sum up, the concentration of Al3+ in the environment is a key factor affecting the absorption of aluminum by ectomycorrhizal fungi. At a certain concentration of Al3+, ectomycorrhizal fungi can increase the absorption of nutrient elements N, P, and K, improve the activity of mycelial enzymes, reduce the content of MDA, alleviate aluminum toxicity, and enhance their viability under aluminum stress, providing a theoretical basis for screening excellent strains with strong resistance (or tolerance) to aluminum. Reference | Related Articles | Metrics | Comments（0）

Select

Effects on Growth and Physiological Characteristics of Camellia semiserrata and C. crapnelliana Seedlings under Different Shadings CHEN Xue-mei, LIN Wei, TU Zhi-jin, WANG Bing-qing, XUAN Zu-ying, HE Jian-qiang, TIAN Xue-qin Subtropical Plant Science    2023, 52 (2): 101-107.   DOI: 10.3969/j.issn.1009-7791.2023.02.003 Abstract （151）      PDF（pc） （542KB）（96）       Knowledge map    Save Two-year-old seedlings of Camellia semiserrata and C. crapnelliana were used as experimental materials and treated with full light (CK), 30% shading, 50% shading and 70% shading, in order to observe the effects of shading on the growth, biomass allocation and some physiological and biochemical indexes of the seedlings. The results showed that 30% shading could significantly increase the height net growth and total biomass of C. semiserrata seedlings, the contents of chlorophyll a and chlorophyll b were significantly increased, but SOD, CAT activities and Pro content were not significantly different from those of CK group, but MDA content was significantly decreased. Under 30% shading, the plant height and ground diameter of C. crapnelliana seedlings were significantly increased, while the contents of chlorophyll a, chlorophyll b, Pro and SOD enzyme activity were not different from those of the full light group, but the content of MDA was significantly decreased. The results showed that the optimal growth condition of the two Camellia seedlings under the experimental treatment was 30% shading. In addition, under 50% shading condition, although the ground diameter growth of C. crapnelliana seedlings was inhibited, its high growth was not significantly different from that of CK group, and the contents of chlorophyll a and chlorophyll b, and activity of CAT were significantly increased, while the contents of Pro and MDA were significantly decreased. The results indicated that the C. crapnelliana seedlings could adapt to the low light environment with 50% shading by adjusting chlorophyll content, CAT activity and Pro content. Therefore, C. semiserrata seedlings have a more stringent demand for light, which is controlled at 30% shading, while C. crapnelliana seedlings can adapt to a more shaded environment (50% shading) in production and greening applications. Reference | Related Articles | Metrics | Comments（0）

Select

Comparison of Physiological and Biochemical Properties of Storage Roots Between High and Low Starch Cultivars of Cassava during the Critical Growth Period CAI Zheng, SUN Jin-liang, LIU Kun-hang, LI You-zhi, FAN Xian-wei Subtropical Plant Science    2023, 52 (2): 85-92.   DOI: 10.3969/j.issn.1009-7791.2023.02.001 Abstract （150）      PDF（pc） （745KB）（284）       Knowledge map    Save This study explored the differences and dynamic changes in the main physiological and biochemical characteristics of roots in high and low starch cultivars of cassava (Manihot esculenta) at key developmental stages (storage root formation and bulking), providing a theoretical basis for cassava management and breeding improvement. Storage root samples of high starch cassava varieties ‘FX01’ and ‘KU50’ and low starch cassava varieties ‘SC124’ and ‘9I’ were selected at 120, 130, 140, 151 and 165 days after planting, respectively. The physiological and biochemical indexes of starch, soluble sugar, abscisic acid (ABA), protective enzyme and ROS were measured and analyzed. The results showed that the soluble sugar content in the cortex of cassava was always higher than that in starch storage area. The soluble sugar content of the high starch varieties increased rapidly during the root expansion period of storage, and showed higher antioxidant enzyme activity and lower ROS and MDA content. ABA content of high starch varieties was lower than that of low starch varieties. Analyzing the effects of these physiological and biochemical parameters on starch accumulation, it was found that the distribution of soluble sugar in low starch and high starch varieties was consistent with the law of starch synthesis after transportation. The starch accumulation rate in high starch varieties was always higher than that in low starch varieties. Their higher antioxidant enzyme activities and lower ROS and MDA content were conducive to the starch accumulation in cassava storage roots. Reference | Related Articles | Metrics | Comments（0）

Select

Responses of Nicotine Key Enzyme Activities and Gene Expression to Compound Fertilizer Dosage and its Proportion in Basal Fertilizer ZHOU Jun-cheng, GUO Ya-li, CHEN Fa-yuan, ZHANG Heng, ZHU Di, PENG San-xi, GAO Huan-ye Subtropical Plant Science    2022, 51 (5): 331-339.   DOI: 10.3969/j.issn.1009-7791.2022.05.001 Abstract （149）      PDF（pc） （376KB）（149）       Knowledge map    Save In order to explore the effect of tobacco-specific compound fertilizer dosage and base fertilizer ratio on the activities of nicotine metabolism key enzymes in roots, i.e. ODC, ADC, MPO, PMT and QPRT, and the expression of genes NtODC and NtADC in leaves of Nicotiana tabacum plants were carried out. In this experiment, a field test was adopted, using ‘Yunyan 87’ as the material, and 3 levels of tobacco-specific compound fertilizer dosage (6.25 kg·667 m–2, 8.25 kg·667 m–2and 10.25 kg·667 m–2) and 3 basal fertilizer ratios (50%, 60% and 70%). The results showed that the effects of different compound fertilizer amounts and basal fertilizer ratios on nicotine key enzyme activities and gene expression were different in each growth period; before the peak period, different compound fertilizer amounts and basal fertilizer ratios had different effects on the activities of ODC, ADC, PMT and QPRT. The significance analysis did not reach a significant level, while the activity of MPO before the peak period generally showed a downward trend with the increase of the compound fertilizer amount, and showed a single peak change with the increase of the base fertilizer ratio. After sprouting, the activities of ODC and MPO performed better at high compound fertilizer rates, and the more suitable basal fertilizer ratios were 50% and 60%, respectively; ADC activity was better with conventional compound fertilizer dosage, and for the same compound fertilizer rate, the ADC activity was higher when the base fertilizer ratio was 50% in general; while the PMT activity was better when the base fertilizer ratio was 70% under the conventional and high compound fertilizer dosages; The activity of QPRT decreased with the increase of base fertilizer proportion under conventional compound fertilizer, but increased with the increase of base fertilizer proportion under high compound fertilizer dosage. The expression levels of nicotine genes NtADC and NtODC in the prosperous and budding periods were generally higher under high compound fertilizer dosage and low basal fertilizer ratio, followed by conventional fertilization. Reference | Related Articles | Metrics | Comments（0）

Select

Chloroplast Genome Characteristics and Phylogeny of the Genus Lycoris (Amaryllidaceae) FU Wen-fo, XIAO Tao, ZHANG Yong-hong Subtropical Plant Science    2023, 52 (4): 271-286.   DOI: 10.3969/j.issn.1009-7791.2023.04.001 Abstract （144）      PDF（pc） （6727KB）（1899）       Knowledge map    Save In this study, the basic characteristics and phylogenetic relationship of chloroplast genome sequences from 17 species of Lycoris were analyzed. The results showed that the chloroplast genomes of Lycoris had a typical quadripartite circle structure with a total length of 158 335–158 761 bp and a total GC content of 37.7%–37.8%. The number of genes was 133–137, and the number of protein-coding genes, rRNA genes, and tRNA genes were identical in different species. There were 41–57 interspersed repeats and 66–74 SSR loci detected, among which single nucleotide A/T repeats were the most frequent. Codon preference analysis showed that the number of coding codons ranged from 26 448 to 26 740. The RSCU values of 31 codons were greater than 1 and the third base of 29 codons were A or U, which indicated that Lycoris plant preferred codons ended with A or U. The results of chloroplast genome alignment and collinearity analysis exhibited highly conservative with no rearrangement and inversion observed. Higher variation frequency of non-coding regions versus coding regions was detected. The IR boundary analysis showed that rpl22, rps19 and ndhF genes in some species crossed the JLB or JSB boundary due to the contraction and expansion of the IR region. Nucleotide polymorphism analysis detected 7 highly variable genes or intergenic regions, trnG-UCC, atpF-atpH, psbC-trnS-UGA-psbZ, trnG-GCC-psbZ, rpl32, trnL-UAG- rpl32-ccsA, rps15-ycf1, which could be used as molecular markers for phylogenetic analysis. The molecular phylogenetic tree showed that all Lycoris species clustered closely and formed a monophyly, which supported the independent taxonomic status of the genus. Two traditional subgenera, Symmanthus and Lycoris, didn’t form monophyly separately, although distinguished in stamen and corolla. And it was still doubtable on the intrageneric circumscription based on the relative position of stamens and perianth. In addition, the phylogenetic analysis supported the perspective of the hybrid origin of L. anhuiensis, L. houdyshelii, L. straminea and L. rosea, with potential maternal species recommended. Reference | Related Articles | Metrics | Comments（0）

Select

Effects of Combined Application of Nitrogen, Phosphorus and Potassium on the Growth Characteristics of Flue-cured Tobacco Seedlings LEI Gang, LIAN Yun, LIU Jin-liang Subtropical Plant Science    2022, 51 (5): 351-361.   DOI: 10.3969/j.issn.1009-7791.2022.05.004 Abstract （142）      PDF（pc） （465KB）（527）       Knowledge map    Save The effects of nitrogen, phosphorus and potassium fertilizers and combined application on the growth of flue-cured tobacco seedlings were studied and the fertilization technology of flue-cured tobacco seedlings was optimized in this paper. Using floating seedlings of the new flue-cured tobacco cultivar Guiyan 202 as the test material, the effects of different fertilization treatments on the growth of flue-cured tobacco seedlings were studied by N, P2O5, K2O single-factor random block test and three-factor five-level quadratic regression almost orthogonal rotation combination test. The results showed that N, P2O5, K2O and their combined application treatments had significant effects on the growth indexes of flue-cured tobacco seedlings. However, the concentrations of P2O5 and K2O had no significant effects on the number of leaves; the higher N concentration, the more significant the effect on the leaf area of tobacco seedlings. The higher N concentration, the larger leaf area in the early stage of tobacco seedlings. While the concentrations of P2O5 and K2O had little effect on the leaf area, and with the growth of tobacco seedlings, the leaf area and the concentrations of P2O5 and K2O first increased and then decreased; Compared with the concentration of N, P2O5 and K2O, it first increased and then decreased. The reasonable ratios of N, P2O5 and K2O could improve the leaf number, stem diameter, seedling height and leaf area of flue-cured tobacco seedlings, and promote the growth of flue-cured tobacco seedlings. From the above growth indexes, the combination treatment of high nitrogen and medium phosphorus and potassium had the best effect in this experiment, when the concentrations of N, P2O5 and K2O in the nutrient solution were 10.50–12.00 mmol·L–1, 4.50–5.25 mmol·L–1 and 10.50 mmol·L–1, respectively. Reference | Related Articles | Metrics | Comments（0）

Select

Pecteilis cambodiana, a New Record Species of Orchidaceae from China MA Liang, LIN Gui-can, YU Yu-yun, LIU Zhong-jian, LAN Si-ren Subtropical Plant Science    2023, 52 (1): 67-69.   DOI: 10.3969/j.issn.1009-7791.2023.01.010 Abstract （141）      PDF（pc） （1041KB）（131）       Knowledge map    Save Pecteilis cambodiana (Gagnep.) Aver. (Orchidaceae) was reported as a new record from China. Its feature description and color pictures were provided. The voucher specimens were deposited in herbarium of College of Forestry, Fujian Agriculture and Forestry University (FJFC). Reference | Related Articles | Metrics | Comments（0）

Select

Regulation of Leaf Functional Traits and Plasticity on the Growth of Hedyotis caudatifolia in Different Habitats WEI Lan-ying, ZENG Chun-yang, YANG Xiao-lan, HUANG Dao-jing Subtropical Plant Science    2024, 53 (2): 100-112.   DOI: 10.3969/j.issn.1009-7791.2024.02.002 Abstract （136）      PDF（pc） （3264KB）（1539）       Knowledge map    Save Most previous researches of plant adaptability to environmental changes were conducted using functional traits at species level. However, one species might displayed distinct ecological strategies in different habitats. Therefore, it’s important to analyze the intraspecific variation of leaf functional traits and their plasticity for understanding the environmental adaption mechanisms and survival status of plant individuals. The present study determined the plant height, biomass and leaf functional traits such as morphological, stomatal, anatomical and chemical characters of medicinal plant Hedyotis caudatifolia from four habitats (grassland, shrub-grassland, forest edge and understory) in Mt. Dayao of Guangxi. Results showed that functional traits varied apparently among different habitats. Among all leaf functional traits, potential conductance index (PCI) exhibited the highest coefficient of variation (CV), while CV of leaf carbon content, looseness of leaf spongy tissue, spongy mesophyll thickness and leaf thickness were lower. Plasticity index (PI) ranked inconsistently with CV for some traits, i.e. potential conductance index displayed the highest PI, followed by mass-based chlorophyll content, leaf area, specific leaf area and ratio of leaf palisade to spongy mesophyll thickness, etc. Principal component analysis (PCA) indicated that the variations of leaf functional traits were closely correlated with light utilization, water conductivity and nutrient strategies, dominated by light adaptation. Analysis of Variable Importance in Projection (VIP) revealed that anatomical structure contributed to the most variances of plant height and biomass, followed by stomatal characters; chemical traits accounted for the least in the contribution rates. The changes of leaf functional traits and their syndromes of H. caudatifolia facilitated itself in utilizing natural environmental resources such as light and water. Thus, it was capable of coping with disadvantages in different habitats, and finally promoted the plant growth and biomass accumulation, which enabled its individuals to compete with other plants in the community for the further survival and reproduction. The study will provide references for the assessment of survival status and safety risk of H. caudatifolia under degraded habitats. Reference | Related Articles | Metrics | Comments（0）

Select

Chemical Constituents and Antibacterial and Anti-inflammatory Activities of Ethanol Extracts from Cinnamomum burmanni Leaves SU Xin-ke, YAO Min, YANG Lei, WU Peng, JIN Jing-wei, XIONG Ping, HUANG Jun-ming, LI Dong-li Subtropical Plant Science    2023, 52 (5): 381-390.   DOI: 10.3969/j.issn.1009-7791.2023.05.002 Abstract （135）      PDF（pc） （833KB）（46）       Knowledge map    Save Modern separation techniques such as D101 macroporous resin, MCI macroporous resin, positive and negative silica gel, gel column chromatography and semi-preparative high performance liquid chromatography were used to separate, purify and identify the chemical components in 95% ethanol extracts of Cinnamomum burmanni leaves from Meizhou, Guangdong Province. At the same time, the antibacterial activities of the compounds were determined by microplate method, and their anti-inflammatory activities were determined by LPS-induced BV2 cells. The results showed that 25 compounds were isolated and identified from the 95% ethanol extracts of C. burmanni leaves, including flavonoids (1–3), lignans (4–6), simple phenylpropanoids (7–16), coumarins (18), benzoquinones (20–21), sesquiterpenes (22–24) and indole alkaloids (25). Except for compounds 1, 2, 5 and 18, all of them were isolated from C. burmanni for the first time. The results of antibacterial experiments showed that compounds 20 and 21 showed good antibacterial activity against Staphylococcus aureus, with MIC of 32 μmol·L–1, while MBC of compound 20 was 64 μmol·L–1, and MBC of compound 21 was 32 μmol·L–1. The results of antibacterial kinetics also showed that compound 21 had better antibacterial activity than compound 20. The results of anti-inflammatory experiments showed that compounds 5, 6 and 7 could inhibit the production of NO in BV2 cells induced by LPS, and showed good anti-inflammatory activity, the IC50 values were 7.85 μmol·L–1, 24.97 μmol·L–1 and 7.49 μmol·L–1, respectively. Reference | Related Articles | Metrics | Comments（0）

Select

Effects of 60Co-γ Irradiation on Physiological Indicators of Camellia drupifera ZHA Qian-hui, HUANG Jiu-xiang, LÜ Ming-tao, GONG Yong-jun, HUANG Yong-fang Subtropical Plant Science    2023, 52 (5): 404-407.   DOI: 10.3969/j.issn.1009-7791.2023.05.005 Abstract （135）      PDF（pc） （695KB）（30）       Knowledge map    Save In order to study the physiologic changes of Camellia drupifera seedlings after 60Co-γ irradiation, the authors did a 60Co-γ by 0 Gy, 40 Gy, 50 Gy, 60 Gy and 70 Gy irradiation of seeds from two excellent materials C. drupifera ‘Fanlong 3’ and ‘Fanlong 5’ and measured chlorophyll content, soluble sugar content, soluble protein content, peroxidase (POD), superoxide dismutase (SOD) in leaves. The result showed that the influence on physiologic index of C. drupifera of 60Co-γ irradiation by various kinds dose was different. The chlorophyll content of 50 Gy group of ‘Fanlong 3’ and ‘Fanlong 5’ were significantly lower than those of other groups (P<0.05). The change tends of soluble sugar content on ‘Fanlong 3’ and ‘Fanlong 5’ were different which showed that the 60 Gy of ‘Fanlong 3’ was relatively low and the 70 Gy of ‘Fanlong 5’ was relatively low. The change tends of soluble protein content on ‘Fanlong 3’ and ‘Fanlong 5’ were the same which showed that the soluble protein contents of 60 Gy treatment were relatively low. The change tends of antioxidant enzyme activity on ‘Fanlong 3’ and ‘Fanlong 5’ were different. The SOD activity of high dose group (60 Gy, 70 Gy) was relatively low and the POD activity of 40 Gy treatment was relatively high in ‘Fanlong 3’. The SOD activity of control group was relatively low and that of 70 Gy treatment was relatively high in ‘Fanlong 5’. The POD activity of 60 Gy treatment was relatively low and that of 50 Gy treatment was relatively high in ‘Fanlong 5’. Reference | Related Articles | Metrics | Comments（0）

Select

Physiological Characteristics of Flower Bud Differentiation in Erythrina sykesii under Drought Stress LIU Bei-ning, CHEN Fa-xing Subtropical Plant Science    2024, 53 (2): 113-119.   DOI: 10.3969/j.issn.1009-7791.2024.02.003 Abstract （135）      PDF（pc） （597KB）（79）       Knowledge map    Save In order to explore the physiological characteristics of drought stress-induced flower bud differentiation in Erythrina sykesii, the physiological response of E. sykesii to drought stress and its flowering induction characteristics were analyzed. The physiological indicators such as the contents of photosynthetic pigments, soluble sugar, malondialdehyde and endogenous hormones, and the activities of superoxide dismutase (SOD) and peroxidase (POD) in the leaves of 8-year-old E. sykesii under drought stress were determined. The results showed that the photosynthetic pigment and endogenous hormone contents and antioxidant enzyme activities in the leaves of E. sykesii exhibited a trend of first increasing and then decreasing with the increasing degree of drought stress. The contents of chlorophylls and carotenoids, starch, and soluble sugar, IAA, GA3 and ZR in the leaves reach their maximum values under mild drought stress. The SOD, POD, catalase (CAT) activities and soluble protein content reach their maximum values under moderate stress. The contents of malondialdehyde, ABA, and carbon nitrogen ratio all significantly elevated along with the increasing degree of drought stress. The above physiological indexes showed that the flowering rate of branches increased significantly under drought stress, of which the effect of the moderate stress was the most obvious. The results indicated that the plant cell damage could be alleviated by increasing ABA content and antioxidant enzyme activity, and the flower bud differentiation and flower formation rate of branches were promoted under moderate drought stress. Reference | Related Articles | Metrics | Comments（0）

Select

Quantitative Characteristics of Nitrogen, Phosphorus and Potassium in Leaves of Typical Chinese Fir Forests in Shimen National Forest Park and Their Correlation with Soil Fertility LAI Wu-ting, MA Hong-yan, ZHANG Hua, ZHU Zi-piao, MO Zhi-an, ZHANG Hui-lin Subtropical Plant Science    2022, 51 (5): 362-367.   DOI: 10.3969/j.issn.1009-7791.2022.05.005 Abstract （134）      PDF（pc） （474KB）（99）       Knowledge map    Save In order to explore the relationship between leaf nitrogen (N), phosphorus (P), potassium (K) contents and soil pH, organic matter, N, P, K contents of typical Chinese fir forest in Shimen National Forest Park of Guangzhou City, different ages and slope positions of Cunninghamia lanceolata forest were selected as the research object in the park. And the correlation between the nutrition of Chinese fir leaves and the content of corresponding nutrient elements in soil was also analyzed. The results showed that the order of N, P and K contents in different stand ages was N > K > P. The contents of N in near mature and mature forests were higher than those in middle aged forests, but K contents were lower in middle aged forests. There was no significant difference in phosphorus content among different forest ages. The N, P and K contents of Chinese fir leaves at different slope positions showed no regularity and no significant difference. The contents of N, P and K in Chinese fir leaves at the same slope position were consistent with the contents of N, P and K in soil at the corresponding slope position. The ratio of N:P of Chinese fir leaves increased with the increase of the forest age, while the N:K and P:K ratios increased first and then decreased with the increase of the forest age. There were significant differences in N:P ratio between middle-aged forests and mature forests, and significant differences in N:K and P:K ratios between middle-aged forests and near-mature forests. The N:P ratio of Chinese fir leaves at different stand ages and slope positions were all less than 14, and the growth of Chinese fir in the experimental area was limited by N but not by K. The results of correlation analysis showed that there was a significant positive correlation between total phosphorus and total nitrogen and potassium, negative correlation between soil pH value and nitrogen, phosphorus and potassium contents in Chinese fir leaves, positive correlation between soil organic matter and nitrogen, phosphorus and potassium contents in Chinese fir leaves, and positive correlation between soil nitrogen, phosphorus and potassium contents and corresponding elements content in Chinese fir leaves. Reference | Related Articles | Metrics | Comments（0）

Select

Diversity and Geographical Pattern Analysis of Wild Lamiaceae Species in China CHANG Meng-lin, CHEN Xin-yan, CHEN Shi-pin, MA Liang Subtropical Plant Science    2024, 53 (3): 234-242.   DOI: 10.3969/j.issn.1009-7791.2024.03.007 Abstract （134）      PDF（pc） （1475KB）（577）       Knowledge map    Save Based on the taxonomic database and a large amount of literature, this study takes administrative counties as the basic unit to sort out the wild Lamiaceae distributed in Chinese Mainland. The species diversity, genus flora, and distribution were analyzed, resulting in the establishment of a geographical distribution dataset for wild Lamiaceae in China. As of January 2024, 100 genera and 1,387 species (including subspecies and varieties) were recorded. Among these, only Scutellaria, a genus with more than 150 species. The genera with species numbers between 101 and 150 include Salvia with 137 species and Isodon with 106 species. Thirty-six genera contain between 6 and 50 species, totaling 733 species, representing the main component of wild Lamiaceae in China. Fifty-nine genera have 5 or fewer species, totaling 119 species, accounting for 8.58% of the total number of wild Lamiaceae species in China. Thirty-three genera are represented by only one species in China, indicating a high genus diversity but low species richness within genera. The primary genera are Scutellaria, Salvia, and Isodon, with a total of 394 species, accounting for 28.62% of the total species. Wild Lamiaceae are distributed across all provinces, autonomous regions, and municipalities in China, primarily in the eastern Himalayas, the mountains of western Hubei and eastern Chongqing, southeastern Tibet-northwestern Yunnan, and the mountains of eastern Yunnan-western Guangxi. The highest species richness is found in Yunnan Province (658 species) and Sichuan Province (532 species). The flora of the genus was diverse. According to Wu Zhengyi's classification of the distribution areas of Chinese seed plant genera, the 100 genera of wild Lamiaceae in China belong to 15 typical types and 12 variant types. Tropical and temperate floristic elements dominate. Genera related to tropical flora account for 39 genera and 548 species, showing a significant predominance. Temperate elements follow, with six genera exhibiting a Mediterranean distribution and two genera exhibiting disjunct distributions between East Asia and North America. Wild Lamiaceae in China are functionally diverse, mainly categorized into medicinal plants, aromatic plants, nectar plants, wild vegetables, ornamental plants, and oil plants. Although some Lamiaceae groups have been explored for their value, many groups remain under-researched, warranting further mechanism research and utilization. Reference | Related Articles | Metrics | Comments（0）

Select

Variation Analysis of Leaf Phenotypic in Different Provenance of Castanopsis hystrix LI Na, YANG Yuan-mu, LI Zi-yue, WEI Rui-yan, XU Fang, CHEN Xin-yu, ZHU Bao-zhu, ZHANG Wei-hua Subtropical Plant Science    2023, 52 (4): 318-326.   DOI: 10.3969/j.issn.1009-7791.2023.04.006 Abstract （133）      PDF（pc） （750KB）（80）       Knowledge map    Save The leaf phenotypic variation characteristics and geographical variation rules of different populations of Castanopsis hystrix were studied in this paper. A total of 229 clones of 17 representative populations in the germplasm gene bank were selected as the research objects. Five phenotypic traits of leaves were selected for variance analysis, correlation analysis and cluster analysis to explore the morphological variation of leaves in different populations of C. hystrix and its relationship with geographical and climatic factors. The variation ranges of leaf area, leaf length, leaf width, leaf index and petiole length of different populations were 10.9–17.9 cm2, 7.5–9.3 cm, 2.2–2.9 cm, 3.3–3.6 and 0.39–0.67 cm, respectively, and the coefficient of variation among characters was 3.2% – 15.6%. There were significant differences between populations and within populations, and the mean variance component percentage within populations (48.404%) was significantly higher than that between populations (11.423%). The variation within populations was the main source of phenotypic variation of C. hystrix leaves. Leaf area, leaf length and leaf width were positively correlated, and leaf width, leaf area and petiole length were negatively correlated with leaf index. Leaf length, leaf width and leaf area were significantly or extremely significantly negatively correlated with longitude, and were significantly positively correlated with altitude. At Euclidean distances 15, 17 populations could be clustered into 3 groups. The variation of leaf phenotypic traits in C. hystrix was abundant, and leaf length, leaf width and leaf area showed a geographical variation pattern dominated by longitude and altitude. With large longitude and low altitude, the leaves are relatively short and small. Reference | Related Articles | Metrics | Comments（0）

Select

Studies on Diversity of Lycophytes and Ferns in the Chebaling National Nature Reserve, Guangdong Province ZHANG Ying-ming, WANG Ting, DENG Shuang-wen, CUI Yu-wen, TAN Hai-rong, CHEN Hong-feng Subtropical Plant Science    2024, 53 (3): 243-256.   DOI: 10.3969/j.issn.1009-7791.2024.03.008 Abstract （129）      PDF（pc） （5121KB）（1170）       Knowledge map    Save In order to comprehensively document the plant diversity within the Chebaling National Nature Reserve in Guangdong and to provide data support for the long-term monitoring, conservation management, and public education regarding biodiversity within the reserve, we conducted field surveys and specimen collection of ferns and lycophytes using transect methods from June to August 2023. A statistical analysis of the species composition, floristic characteristics, and resource status of ferns and lycophytes within the reserve was carried out by integrating literature data. The results indicated: (1) A total of 231 species (including infraspecific taxa) of ferns and lycophytes belonging to 75 genera and 25 families distributed in the nature reserve, among them 64 species from 11 families and 28 genera were new records in the area. (2) There were six monotypic families and 38 monotypic genera, accounting for 24.00% and 50.67% of the total families and genera, respectively, which reflected the ancient and primitive nature of the ferns and lycophytes flora in the area. (3) Ferns and lycophytes composition in this area are derived from multiple origins. The tropical component ranked at the family (60.00%) and genus (68.00%) levels, while at the species level, the subtropical component (54.98%) held prevalence, followed by the tropical component (29.87%). It aligns with the characteristics of subtropical flora, demonstrating a certain degree of features associated with tropical flora. (4) Compared with adjacent nature areas, Chebaling Nature Reserve shows the closest relationship with Qingyunshan Provincial Nature Reserve in Guangdong province. The similarity indices of species, genera, and families (Jaccard/Krober coefficients) stand at 92.00/96.00, 73.42/85.33, and 42.42/62.86, respectively. This suggests a probable connection to their geographical proximity and similar climatic features. (5) Within the nature area, ferns and lycophytes resources are abundant, including six edible species, 145 medicinal species, 141 ornamental species, and 32 species with industrial value. These resources provide rich opportunities for the future rational development and effective utilization of plants. Reference | Related Articles | Metrics | Comments（0）

Select

Effects of Meteorite Water on Seed Germination and Physiological Characteristics of Pea Seedlings LIU Bei-ning, LI Liu-min, CHEN Fa-xing Subtropical Plant Science    2023, 52 (2): 119-124.   DOI: 10.3969/j.issn.1009-7791.2023.02.005 Abstract （128）      PDF（pc） （789KB）（139）       Knowledge map    Save The effects of meteorite water on the seed germination and seedling growth and physiological characteristics of pea were studied. The results showed that the meteorite contained 10 essential mineral elements and 2 beneficial elements for plant growth. Meteorite water could promote the growth and development of pea seedlings. The germination rate of peas in the meteorite boiling water treatment group reached 79.2%, significantly higher than that in the soaking water treatment and control; The plant height and root length of pea seedlings treated with meteorite boiling water increased by 24.03% and 22.13%, and the fresh weight of aboveground and underground parts increased by 11.23% and 24.22% compared to the control, respectively; Meteorite boiling water significantly increased the content of soluble sugar, protein, total chlorophyll, peroxidase activity of pea seedlings, and also significantly increased the B, Fe, V, Sr, Sn content of pea sprouts. Meteorite boiling or soaking could alter the physical and chemical properties of water, and the effects of boiling water on seed germination and seedling growth was obviously better than soaking water. Reference | Related Articles | Metrics | Comments（0）

Select

Community Types and Species Diversity of Picrasma quassioides Communities in Guangxi LI Jin-hua, PAN Xiu-lian, LIU Qin, WEI Qiu-lan, ZHU Chang-san, CHEN Rong, HUANG Hua-xi Subtropical Plant Science    2023, 52 (2): 135-142.   DOI: 10.3969/j.issn.1009-7791.2023.02.007 Abstract （128）      PDF（pc） （560KB）（1253）       Knowledge map    Save A sample plot survey was used to investigate the communities of Picrasma quassioides, and the habitat characteristics, species composition, floristic composition and species diversity were analyzed. The results showed that 255 species in 207 genera and 85 families of vascular plants were found in 10 plots, and there are abundant species in the P. quassioides community. The main dominant families were Euphorbiaceae, Fabaceae, Gramineae, Moraceae, etc. The floristic types of seed plants in the community were divided into 14 types, with pantropic and tropical Asian elements as the main components, with tropical elements accounting for 72.73% of the total genera, and the community had obvious tropical properties. The community was divided into four types. P. quassioides grew vigorously in community Ⅱ and community Ⅳ, and was the dominant species in the tree layer. Community Ⅱ and community Ⅳ were the ideal habitat. The Margalef index, Shannon Wiener index and Pielou index showed that the shrub layer was larger than the tree layer. The three diversity indexes of tree and shrub layer in community Ⅱ were the highest, while Margalef species richness index of tree layer in community Ⅳ was the higher, while Shannon-Wiener diversity index was the lowest. The root budbreak ability of P. quassioides is stubborn, and the closure measures have positive significance for the restoration of P. quassioides population. The results can provide basic information for the protection and cultivation of P. quassioides resources. Reference | Related Articles | Metrics | Comments（0）

Select

Differences in Microbial Communities among Ganpu Tea from Different Years HONG Pei-yu, WANG Ya-yu, WU Hong Subtropical Plant Science    2024, 53 (3): 191-200.   DOI: 10.3969/j.issn.1009-7791.2024.03.001 Abstract （127）      PDF（pc） （5289KB）（666）       Knowledge map    Save Ganpu tea is made by removing the flesh of fresh Citrus reticulata 'Chachiensis' and filling them with Yunnan Pu-erh tea. It has both the aroma of Pericarpium Citri Reticulatae 'Chachiensis' and the fragrance of Pu-erh tea. In this study, Ganpu teas were separated into tea leaves and peels with different aging times for microbiome analysis. It was found that the microbial composition of the peels and tea leaves of Ganpu tea was more similar in the early aging stage, while the differences became more pronounced in the later aging stage. In tea leaves of Ganpu tea aged at different times, the dominant bacterial genera included Bacillus, Staphylococcus, and Blastobotrys. In the peel of Ganpu tea aged at different times, the dominant bacteria aged for 0 to 1 year include Staphylococcus, while the dominant bacteria aged for the other three times are Acinetobacter. In the fungal community, the dominant fungal genera in aged 0, 1, and 5 year old peels, as well as five different aged Ganpu tea leaves, are all Blastobotrys. The dominant fungal genus in aged 7 and 9 year old Ganpu tea peels is Xeromyces. Among the tea leaves or peels of Ganpu tea at different aging times, the majority of samples (over 75% of the abundance) shared the same Amplicon Sequence Variants (ASVs). Correlation analysis based on these shared ASVs revealed a significant positive correlation between Aspergillus and other microorganisms in the network, although its abundance was not particularly high in Ganpu tea at different aging times. Reference | Related Articles | Metrics | Comments（0）

Select

Effects of Exogenous Strigolactone on Axillary Bud Elongation and Expression of Genes Related to the Strigolactone Metabolic Pathway in Tobacco TIAN Hui-yuan, TANG Bo-xi, WANG Yuan-xiu, LIU Fan, GUO Kai-yang, LIU Guo-qin Subtropical Plant Science    2023, 52 (5): 369-380.   DOI: 10.3969/j.issn.1009-7791.2023.05.001 Abstract （125）      PDF（pc） （6086KB）（1208）       Knowledge map    Save The effects of strigolactone (SL) on the elongation of tobacco axillary buds and the expression of genes related to its metabolic pathway were carried out, to explore the relationship between SL and the growth of tobacco axillary buds and the expression changes of SL-related genes during the elongation of tobacco axillary buds, which could establish a theoretical basis for the molecular regulation mechanism of SL in regulating the growth of tobacco axillary buds. This study investigated the developmental changes of tobacco axillary meristematic tissues after decapitation, and observed the various genes associated with SL metabolic pathway of tobacco axillary buds by treatment with GR24, an artificial synthetic analogue of SLs, using paraffin sectioning and transcriptome technique. The results showed that the axillary meristem of tobacco was composed of three cell regions: central blast region (CM), peripheral meristem (PM) and costal meristem (RM), among which the CM was located at the top of the axillary meristem, which could form PM and RM through the division of basal and lateral cells, the PM was located on both sides of the axillary meristem, and the RM was located below the CM region and the inner side of the PM region. The cell volume and arrangement of the three cell regions were different, indicating that there were differences in cell division in each part of the meristem. After axillary meristem development to form axillary buds, it was found that GR24 could inhibit the elongation of axillary buds at 1–3 nodes by exogenous application of different plant growth regulators, while BR could promote the elongation of axillary buds. Transcriptome analysis revealed that SL could affect the expression of genes related to its own metabolic pathway, SL induced the expression of D27, D14, DAD2 and SMAX1-LIKE4 compared to CK and TIS-108 [a triazole-type strigolactone(SL)-biosynthesis inhibitor] treatment, whereas, D27, D14 and DAD2 were down-regulated by TIS-108 treatment, the expression of SMAX1 was inhibited by SL, while TIS-108 treatment induced the expression of SMAX1. The axillary buds of tobacco are developed from three hyphal regions of the axillary meristem, while different plant growth regulators have different effects on the axillary buds of tobacco. BR can promote the elongation of the axillary buds of tobacco, while SL can inhibit the elongation of the axillary buds, and the genes related to the metabolic pathway may play an important regulatory role in the elongation of the axillary buds of tobacco. Reference | Related Articles | Metrics | Comments（0）

Select

Species Composition and Floristic Characteristics of Eucalyptus spp. Plantations in Guangxi, South China HUO Chun-lin, ZHANG Zhong-hua, HU Cong, XU Chao-hao, HU Gang Subtropical Plant Science    2023, 52 (5): 424-432.   DOI: 10.3969/j.issn.1009-7791.2023.05.008 Abstract （125）      PDF（pc） （766KB）（819）       Knowledge map    Save Through integrating field surveys and literature data, this study analyzed the species composition and floristic characteristics of vascular plants in Eucalyptus plantation in Guangxi. The results showed that there were 584 species from 369 genera and 120 families of vascular plants in the Eucalyptus plantation of Guangxi. Among them, ferns consisted of 49 species from 28 genera and 15 families, gymnosperms included 6 species from 4 genera and 4 families, and angiosperms comprised 529 species from 337 genera and 101 families. These values account for 38.83%, 18.35%, and 6.37% of the total number of vascular plant families, genera, and species in Guangxi, respectively, indicating relatively low plant diversity in Eucalyptus plantation. Shrubs (201 species) dominated the vegetation, while lianas (91 species) were the least represented. At the family and genus level, this flora was dominated by plurimotypic families, mesotypic family, oligotypic genera and monotypic genera. The vascular plant genera and species tended to be concentrated in plurimotypic families, oligotypic genera and monotypic genera, indicating that the flora had a certain differentiation potential and the composition of families and genera was relatively scattered. The abundant primitive taxa of dominant families, genera, and species indicate that the floristic components had a certain degree of antiquity. There were a total of 14 distribution types and 15 subtypes in the genus distribution area. The ratio of tropical to temperate genera (R/T value) was 5.37, with tropical components being the main component, with pan tropical components being the most dominant. Only 2 genera and 2 species were endemic to China in the flora. Overall, the flora of Eucalyptus plantation in Guangxi exhibited complex composition, apparent tropical characteristics, ancient origins, but lacked unique elements, and had relatively low differentiation. These research findings could be utilized to guide the analysis of plant diversity and floristic characteristics in Eucalyptus plantation in Guangxi. Reference | Related Articles | Metrics | Comments（0）

Select

Effects of Drought Stress on Physiological and Biochemical Characteristics and NtDEGP5 Gene Expression SHU Yan-qi, LUO Jia-jun, JIA Rong-li, DUAN Li-li, MO Ze-jun, LIU Ren-xiang Subtropical Plant Science    2023, 52 (1): 1-8.   DOI: 10.3969/j.issn.1009-7791.2023.01.001 Abstract （124）      PDF（pc） （859KB）（247）       Knowledge map    Save To study the effects of drought stress on physiological and biochemical characteristics and the differential expression of NtDEGP5 gene in different tobacco cultivars, drought resistant variety NC82 and drought sensitive Yunyan 87 were used as materials, and PEG-6000 was used to treat seedlings with drought stress. Fv/Fm, SOD activity, POD activity, MDA content and NtDEGP5 relative expression of two tobacco cultivars were determined after treatment, so as to provide a basis for further research on the function of NtDEGP5 gene. The results showed that drought stress had effects on Fv/Fm, SOD activity, POD activity and MDA content of tobacco seedlings. As the drought continued, Fv/Fm showed a gradual decline trend, while POD, SOD activity and MDA content showed a trend of first increasing and then decreasing. The response of different varieties to drought stress was different. Under drought stress for 0 to 9 hours, the Fv/Fm of Yunyan 87 seedlings decreased more than that of NC82, while the POD, SOD activity and MDA content increased less than that of NC82. The difference was not significant after 12 hours of drought stress. Under drought stress, the expression of NtDEGP5 was different among varieties and organs. The expression levels in roots, stems and leaves of Yunyan 87 seedlings were very low, and that in roots of NC82 seedlings was also very low, but it was high in leaves and stems after treatment for 9–12 h. NtDEGP5 expression in leaves of NC82 was positively correlated with Fv/Fm, POD activity and MDA content. The results showed that Fv/Fm, SOD activity, POD activity and MDA content could be used as drought resistance evaluation indicators of tobacco seedlings. NtDEGP5 gene expression was significantly correlated with drought resistance evaluation indicators. It was speculated that NtDEGP5 had drought resistance function. Reference | Related Articles | Metrics | Comments（0）

Select

Morphological Development of Fruits and Seeds of Aucuba chinensis in Badagongshan National Nature Reserve, Hunan Province LU Xiao-rong, LIU Jiang-tao, YU Ping, LI Long, CHEN Jin-hua, GU Zhi-rong, HE Gang, TIAN Xiang-rong Subtropical Plant Science    2023, 52 (5): 413-423.   DOI: 10.3969/j.issn.1009-7791.2023.05.007 Abstract （124）      PDF（pc） （6736KB）（27）       Knowledge map    Save Using Aucuba chinensis from Badagong Mountain National Nature Reserve as a model, the regularity of fruit and seed development were elucidated basing on monthly observation on fruit/seed length, width, color and embryonic/endosperm morphology. On this basis of observation, the relationship between morphological developmental and ecological factors including annual average temperature and rainfall were analyzed. The results showed that: (1) The total development of fruit took 13 months, which means the process from fruit abscission to falling lasted from April to next April. (2) The variation of fruit length, width and peel thickness manifested a tendency which include four stages as “slow-fast-slow-fast”, and which just like a typical double “S” shaped curve. And the most obvious development of fruit shape index appeared in stage Ⅱ, whose value varied from 2.63 to 1.57. (3) In the developmental process, fruit color kept green in the early and lasted for a long time, and then turn red very quickly, which should attribute to the rapid value decreasing of blue and green color. (4) Embryo and endosperm growth were synchronized with fruit development, and growth stage Ⅰ was the critical stage for embryonic and endosperm development, in which the embryo length/width ratio increased from 1.83 to 2.56; the endosperm developed as a cellular phenotype without a typical pasty stage. (5) At 13 months after corolla drop, about 77% of the embryos were still torpedo shaped. (6) There were significant positive correlation in fruit shape index/embryo ratio and temperature/rainfall (P<0.05), but fruit shape index was more dependent on precipitation (P<0.01) and embryo rate was more dependent on annual average temperature. Based on the above, the fruit development of A. chinensis was characterized by the obvious phenomenon of simultaneous flowering and overlapping fruiting periods. And the limitation of hydrothermal conditions was probably the main reason for the slow development of fruit and seed and the impact on seedling establishment. At the same time, most of the seed embryos were not fully differentiated when the fruits were abscised, which meant that the seed development had to undergo after-ripening, and therefore the seeds were morphologically dormant. Reference | Related Articles | Metrics | Comments（0）

Select

Analysis on Geographical Components and Garden Application Potential of Aromatic Plants in Guangdong Province LIANG Dan, CHEN Jun-ting, WANG Zhen-zhen, JIANG Piao, LI Zhuo-ran, WENG Shu-fei Subtropical Plant Science    2023, 52 (2): 157-163.   DOI: 10.3969/j.issn.1009-7791.2023.02.010 Abstract （123）      PDF（pc） （359KB）（79）       Knowledge map    Save Aromatic plants are important functional plants in urban gardens. This paper analyzes the geographical components of families and genera on the basis of collecting the list of aromatic plants in Guangdong province, and investigates their landscape application. The results show that there are a total of 122 families, 334 genera and 703 species (including varieties and subspecies) of aromatic plants in Guangdong province. The dominant families are Lauraceae, Rutaceae, Zingiberaceae, Labiatae, Compositae, Myrtaceae, Leguminosae and Magnoliaceae. From the perspective of geographical components of plants, 63 families belong to tropical components, accounting for 70.79% of the total number of families. There are 183 genera belonging to tropical components, accounting for 58.84% of the total genera. The analysis of family and genera geographical components show that the tropical components of aromatic plants in Guangdong province are obvious, which is consistent with the geographical location of Guangdong province in the subtropical monsoon region. Among aromatic plants, there are 529 native plants, accounting for 75.25%, and 174 exotic species, accounting for 24.75%, indicating that native plant resources in aromatic plants are abundant. There are 455 kinds of aromatic plants in garden application, but the aromatic characteristics are not clear. It is suggested to further screen excellent aromatic plants, increase the garden application of aromatic woody liana species, and introduce local plants such as Compositae, Labiatae, Umbelliferae, Rutaceae to expand the scope of use of aromatic plants. Reference | Related Articles | Metrics | Comments（0）

Select

Three New Records of Angiosperms to Guangdong Province JIANG Yuan-yuan, LIU Zhong-cheng, PENG Xiu-li, ZHAO Wan-yi, LIAO Wen-bo Subtropical Plant Science    2022, 51 (5): 389-393.   DOI: 10.3969/j.issn.1009-7791.2022.05.009 Abstract （123）      PDF（pc） （445KB）（98）       Knowledge map    Save Three taxa, i.e. Fimbristylis dipsacea var. verruciferai (Maxim.) T. Koyama (Cyperaceae), Camellia?pilosperma S. Y. Ling ex Chang (Theaceae) and Rhomboda?moulmeinensis (E. C. Parish & H. G. Reichenbach) Ormerod (Orchiacaeae) were reported as new records to Guangdong province. The morphological description, and habitat and distribution of above three species were also provided. Reference | Related Articles | Metrics | Comments（0）

Select

Does Phylogenetic Relatedness Affect the Neighborhood Effects among Neighbors in Plant Communities? WANG Li-ping, CHEN Feng-xian, FENG Zhe, YU Xiao-li, SUN Ji-wen, WU Jun-jie Subtropical Plant Science    2023, 52 (3): 242-252.   DOI: 10.3969/j.issn.1009-7791.2023.03.009 Abstract （123）      PDF（pc） （1337KB）（546）       Knowledge map    Save Phylogenetic relationships among neighbors are significant predictors of density dependence in plant communities. However, there is no consistent conclusion about the effect of phylogenetic density dependence . We conducted a comprehensive literature search for the effect of phylogenetic density dependence using the Web of Science, Google Scholar and CNKI by keywords from 1 January 1980 to 1 May 2022. We screened titles, abstracts and full texts of the papers, and 35 publications were selected. The strength of the influence of phylogenetic distance on the interactions between neighboring trees in each study was extracted. There are three effects of phylogenetic relationships on neighborhood effects: phylogenetic negative density dependence (PNDD), phylogenetic positive density dependence (PPDD) and no phylogenetic density dependence, the numbers of whose articles decrease in turn. PNDD was mainly caused by associated competition resources, natural enemies and host-specific pathogens. PPDD was associated with small habitat heterogeneity, habitat filtering and symbiosis with mycorrhizal fungi. However, there are no phylogenetic density dependence due to absence of phylogenetic signal in functional traits and no direct interaction between early plant neighbors. The main reasons for the different results caused by various environmental heterogeneities, research scales, study types, target species and the life history stages of plants in different studies. Therefore, the influence of these factors should be considered as far as possible in the study of phylogenetic density dependence in the future. Reference | Related Articles | Metrics | Comments（0）

Select

Investigation and Analysis of the Diversity of Self-Generated Herbaceous Plants in Three Universities in Lanzhou City AN Qi, LIU Wen-lan Subtropical Plant Science    2024, 53 (3): 257-270.   DOI: 10.3969/j.issn.1009-7791.2024.03.009 Abstract （120）      PDF（pc） （11976KB）（47）       Knowledge map    Save Autogenic plants are essential plant materials for constructing low-cost, low-maintenance, and sustainable conservation-oriented landscapes. To comprehend the current status of autogenic herbaceous plant resources in colleges and universities within Lanzhou City and to apply them more effectively in gardens, the grid sampling approach was adopted to carry out monthly investigations of autogenic herbaceous plants on the campuses of Gansu Agricultural University, Lanzhou University, and the old campus of Lanzhou University of Technology from April to October 2023. The habitats were classified into 4 types, totaling 146 sampling points and 438 sampling plots. The species composition and diversity characteristics were analyzed through calculation methods such as the Shannon-Wiener index and Ducan test. The results indicated that a total of 103 species belonging to 87 genera and 32 families of autogenic herbaceous plants were surveyed in the three universities, among which 61 were native plants, accounting for 59.22%; 21 were invasive alien plants, constituting 20.39%. The life form was predominantly perennial herbs, with a total of 63 species, and 40 were annual and biennial herbs. The top 3 species in terms of species frequency were Taraxacum mongolicum (67.63%), Viola prionantha (55.76%), and Trifolium repens (48.32%), which were abundant in all sampling plots and had certain growth advantages. The dominant families were Compositae and Poaceae, with 44 species belonging to 34 genera. Compositae plants accounted for the highest proportion; among them, Taraxacum mongolicum held significant values within the top 5 in three types of sampling plots such as shade, grassland, and road, and ranked first in the shade sampling plot of Gansu Agricultural University, with an important value of 42.21. From the perspective of spatial pattern, there were more species in shade and grassland habitats, with 59 and 47 species respectively, and their Shannon-Wiener index was in the order of shade > grassland > road > waterside; the Shannon-Wiener index demonstrated a trend of initially rising, then falling, and subsequently rising over the months, with the highest values in June and October, averaging 6.41 and 6.17 respectively. The diversity of each habitat sampling plot was at a relatively high level, which was related to the climate, human disturbance, and management level. The autogenic herbaceous plants on campus exhibit good ecological adaptability and plant landscape effects. Scientific utilization and planning can shape garden plant landscapes with distinctive features and ecological significance. Reference | Related Articles | Metrics | Comments（0）

Select

Acute Toxicity and Genotoxicity Test Study of Extracts from Millettia speciosa Roots ZHAO Yu-jie, CHEN Mei-fen, LI Xin, ZHANG Zi-hong, HUANG Jin-wen, HUANG Jun-ming, ZHANG Kun, LI Dong-li Subtropical Plant Science    2023, 52 (3): 197-202.   DOI: 10.3969/j.issn.1009-7791.2023.03.004 Abstract （119）      PDF（pc） （350KB）（238）       Knowledge map    Save This study evaluated the acute toxicity and genotoxicity of Niudali (Millettia speciosa roots) extracts. The limit method was used in acute toxicity test. The plate incorporation method was used in bacterial revert mutation test, and five strains of Salmonella typhimurium mutant strains TA97a, TA98, TA100, TA102 and TA1535 with histidine nutritional deficiency were counted back. The proportion of bone marrow polychromatic erythrocytes in total red blood cells and the proportion of micronucleus-containing polychromatic erythrocyte were counting in mammalian erythrocyte micronucleus test. Chromosomal aberration test was carried out through Chinese hamster lung (CHL) cell culture technology, the chromosome number and structural changes of CHL cell lines infected with samples were observed. The results showed that the tested samples were practically non-toxic, and the results of bacterial reversion mutation test, erythrocyte micronucleus test and chromosome aberration test were all negative. No acute toxicity or genotoxicity were observed within the dose range of this test for Niudali extracts. Reference | Related Articles | Metrics | Comments（0）

Select

Seed Germination of 14 Wild Functional Ornamental Herbaceous Species from the Northwest Sichuan YANG Ma-jin, HUANG Wen-juan, LI Zhen, TANG Xiao-hua, HAN Ju-lan, YE Chang-hua Subtropical Plant Science    2023, 52 (3): 228-241.   DOI: 10.3969/j.issn.1009-7791.2023.03.008 Abstract （119）      PDF（pc） （398KB）（47）       Knowledge map    Save By different storage methods and different seed treatments, 14 wild functional ornamental herbaceous species occurring in the Northwest Sichuan were used as experimental materials to carry out seed germination, and based on the germination characteristics of each species to explore the suitable storage methods and dormancy removal methods of seed breeding in low altitude areas. The results indicated that: ① Lilium duchartrei, Cynoglossum amabile, Anemone cathayensis, Primula sikkimensis, Aster dolichopodus maintained high vigor and strong germination ability before and after storage, and the seed germination abilities of Primula nivalis, Sinopodophyllum hexandrum, Meconopsis integrifolia were obviously enhanced after storage; ② The effects of different storage methods on seed germination rate of 14 species were significant (P＜0.01), sand storage was superior to dry and low temperature storage, and the germination rate of seeds under sand storage was the highest at low temperature of 3 ℃; ③ The effects of different seed treatments on seed germination rate of 14 species were significant (P＜0.01), and the seed germination rate was most significantly different deal with the treatment of gibberellin solution and 6-BA solution. Gibberellin solution promoted the seed germination, while the 6-BA solution had opposite effect, and mechanical peeling and sulphuric acid treatment significantly reduced the germination rate; ④ The germination process of most plant seeds was shortened obviously by methods of low temperature of 3 ℃ in sand storage, Gibberellin immersion, water immersion and ultrasonic shock. Cynoglossum amabile, Anemone cathayensis, Salvia przewalskii, Anemone rivularis and Aster dolichopodus seedlings were adapted to the natural environment of Chengdu and showed strong adaptability in summer. Reference | Related Articles | Metrics | Comments（0）

Select

Quality Evaluation of Public Welfare Forest in Yingde City TAN Jun-jie, HUANG Hua-rong Subtropical Plant Science    2022, 51 (5): 368-373.   DOI: 10.3969/j.issn.1009-7791.2022.05.006 Abstract （118）      PDF（pc） （739KB）（19）       Knowledge map    Save This study used the analytic hierarchy process to evaluate the forest quality of public welfare forests in Yingde City, Guangdong Province. According to the productivity status, stand stability, and soil conditions, nine indicators such as average diameter at breast height, average tree height, and canopy density were selected, and a public welfare forest quality evaluation index system was established and graded. The results showed that the comprehensive quality grade of the public welfare forest in Yingde was good, and the productivity was excellent. The overall age group of public welfare forests were dominated by young and middle aged forests, and the area of near mature and over mature forests with good ecological benefits were relatively low. The comprehensive forest quality evaluation of public welfare forests in all age groups increased with the increasing of age composition maturity. From the weight of evaluation indicators, the productivity status was the most important indicator to evaluate the impact of public welfare forests. The canopy density, forest community structure and litter thickness were important indicators to evaluate the impact of productivity, stand stability and soil conditions on public welfare forests. It was suggested that in the future construction and management of public welfare forests, the forest structure of public welfare forests should be optimized, and the forest types and age groups should be strategically adjusted. Under the condition of not changing the existing vegetation coverage and canopy density as far as possible, appropriate thinning, replanting, and replanting should be carried out to improve the quality of forests. Long term continuous monitoring of public welfare forests should be carried out to master the dynamic change law of the quality of public welfare forests. Reference | Related Articles | Metrics | Comments（0）

Select

Diversity and Protection Suggestions of National Key Protected Wild Plants in Jingning, Zhejiang Province HE Hai-rong, WU Yao-cheng, JIN Min-zhong, LIN Jian, CHENG Li-bo, XIA Li-min Subtropical Plant Science    2023, 52 (4): 336-342.   DOI: 10.3969/j.issn.1009-7791.2023.04.008 Abstract （116）      PDF（pc） （519KB）（58）       Knowledge map    Save The project team completed the investigation and analysis of national key protected wild plant resources and their diversity in Jingning County by means of literature review and field investigation. According to the investigation, there are 51 species of wild plants in 38 genera and 28 families under state key protection in Jingning, including 1 species of first class and 50 species of second class. The composition and distribution characteristics of the family, genus and species of the state key protected plants were studied, and the endangered status and causes of endangerment were analyzed. At the same time, according to the actual situation of Jingning, the corresponding protection measures were proposed such as strengthening publicity and education to enhance the public protection awareness, strengthening law enforcement to improve the level of protection, strengthening resource investigation to understand the dynamic changes, strengthening the management of natural protected areas to improve the effectiveness of protection. Reference | Related Articles | Metrics | Comments（0）

Select

Photosynthetic Characteristics and Photosynthetic Carbon Sequestration and Oxygen Release Capacity of 11 Mangrove Species ZHANG Liu, ZHAN Qiao-si, GUO Wei, LAI Can, YANG Qiong, ZHANG Hui Subtropical Plant Science    2023, 52 (6): 465-474.   DOI: 10.3969/j.issn.1009-7791.2023.06.001 Abstract （116）      PDF（pc） （880KB）（577）       Knowledge map    Save Mangrove forest is an important part of the global carbon cycle, and the study of the photosynthetic characteristics of different mangrove plants and the difference in carbon sequestration and oxygen release capacity is an important basis for the cumulative assessment and protection and restoration of carbon pool of mangrove biomass. The daily dynamics of photosynthesis rate of mangrove trees and leaf area index were determined by using Li-6800 Portable Photosynthesizer and TOP-1300 Canopy Analyzer, to analyze the daily capacity of carbon sequestration and oxygen release of the target species. Carbon sequestration per unit land area was used as a measure of the CO2 absorption capacity of mangrove plants for cluster analysis to further analyze the main factors affecting the oxygen sequestration capacity of plants. The results showed that: (1) The net photosynthetic rate of six species was unimodal curve, including Cerbera manghas, Kandelia obovata, Heritiera littoralis, Acanthus ilicifolius, Hibiscus hamabo and Volkameria inermis, and the remaining five species showed a bimodal curve due to photosynthetic lunch break, among which Cerbera manghas was the lowest and Volkameria inermis was the highest. (2) In terms of carbon sequestration and oxygen release capacity per unit land area, 3 species of shrubs (Acanthus ilicifolius, Hibiscus hamabo, Volkameria inermis ) and 4 species of trees (Aegiceras corniculatum, Excoecaria agallocha, Heritiera littoralis, Sonneratia apetala ) performed better. (3) The results of the clustering of daily carbon sequestration per unit land area of 11 mangrove species were divided into three categories, the first category included Hibiscus hamabo and Volkameria inermis with the highest daily carbon sequestration, the second category was Aegiceras corniculatum, Excoecaria agallocha, Heritiera littoralis, Acanthus ilicifolius and Sonneratia apetala with medium daily carbon sequestration, and the third category was Cerbera manghas, Thespesia populnea, Bruguiera gymnorhiza and Kandelia obovata with the lowest daily carbon sequestration. (4) The main influencing factors of daily carbon sequestration and oxygen release per unit land leaf area were transpiration rate (Tr) and stomatal conductance (Gs). Reference | Related Articles | Metrics | Comments（0）

Select

Effects of Effective Accumulated Temperature on Antioxidant Enzyme Activities and Accumulation of Functional Substances in Alisma orientale and A. plantago-aquatica LANG Pei-lei, HAN Yun, TIAN Rong, GU Wei, QIU Rong-li, MA Qi-han, WU Wen-qing, LIU Meng-xue, JU Rui-xin Subtropical Plant Science    2023, 52 (3): 183-190.   DOI: 10.3969/j.issn.1009-7791.2023.03.002 Abstract （115）      PDF（pc） （568KB）（49）       Knowledge map    Save The variation range and distribution pattern of effective accumulated temperature in the field growth period were analyzed based on the meteorological data of Jian Zexie (Alisma orientale) and Chuan Zexie (A. plantago-aquatica) in Jian’ou, Fujian Province and Pengshan, Sichuan Province in recent 40 years. We simulated the changes of effective accumulated temperature in two genuine producing areas, and compared the effects of effective accumulated temperature on the activities of antioxidant enzymes and the accumulation of functional substances in Jian Zexie and Chuan Zexie. The results showed that: (1) The effective accumulated temperature range of Jian Zexie and Chuan Zexie were 1044–1340 ℃·d and 602–962 ℃·d, respectively, and there were significant differences in the effective accumulated temperature required at different growth stages; (2) Under the condition of effective accumulated temperature in genuine producing areas, Jian Zexie and Chuan Zexie were more resistant to oxidation and had higher alisol B 23-acetate content in tubers, and the rapid accumulation period appeared on the 105–120 d, while the accumulation of 23-acetyl alisol C in two types of Jian Zexie and Chuan Zexie was opposite, and the content of 23-acetyl alisol C in genuine producing areas was low. These results showed that the effective accumulated temperature could significantly affect the antioxidant capacity and the accumulation of functional substances of Jian Zexie and Chuan Zexie. This study provided a theoretical basis for investigating the genuine causes of Jian Zexie and Chuan Zexie and standardized cultivation and management. Reference | Related Articles | Metrics | Comments（0）