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    Research Progress on the Application of Slightly Acidic Electrolyzed Water in Food Preservation
    DAI Xiao-ze, WANG Chao, LIU Qing-qing, FENG Shu-juan, MING Yan-lin, LIN Ming-you, ZHANG Yan-hui, LIN He-tong, CHEN Yi-hui
    Subtropical Plant Science    2025, 54 (1): 89-99.   DOI: 10.3969/j.issn.1009-7791.2025.01.012
    Abstract21)      PDF(pc) (1224KB)(337)       Save
    Slightly acidic electrolyzed water (SAEW), as an environmentally friendly sanitizer, exhibits characteristics such as low cost, high efficiency, robust environmental protection, strong safety, and non-corrosiveness, which have led to its widespread application in food sterilization and preservation. This paper delves into the preparation principle, key parameters, sterilization mechanism, and application advantages of SAEW. Additionally, it compiles the latest advancements made by scholars globally in recent years concerning the use of SAEW in sterilizing and preserving a variety of foods, including fruits and vegetables, aquatic products, livestock meats, and poultry meats. Furthermore, the paper outlines potential future research directions and breakthroughs in SAEW technology, aiming to serve as a reference for broadening the application scope of SAEW within the food industry.
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    Estimation of Genome Sizes of Pistacia chinensis by Flow Cytometry and Genome Survey
    ZHOU Mei-jun, YIN Yue, ZHANG Yong-hong
    Subtropical Plant Science    2024, 53 (6): 495-502.   DOI: 10.3969/j.issn.1009-7791.2024.06.001
    Abstract80)      PDF(pc) (4084KB)(306)       Save
    Pistacia chinensis, a deciduous tree of the genus Pistacia (Anacardiaceae), is an important oil plant resource for its seeds being rich in oil. It has value in wood production and landscape too. Until now, no relevant information on the whole genome of P. chinensis has been found, which limits its resource utilization and germplasm innovation. To determine the whole genome sequencing strategy of P. chinensis, flow cytometry combined with high-throughput sequencing technology was employed to estimate the genome size of this species. The results were as follows: (1) The genome sizes of P. chinensis were estimated to be approximately 480 Mb and 406 Mb by flow cytometry using Solanum lycopersicum and Arabidopsis thaliana as internal references, respectively. (2) 86 Gb of raw data were produced in next-generation sequencing and approximately 85 Gb of clean data were obtained after filtering. (3) NT database comparison found no exogenous pollution in the sequencing data, with five most math species belonging to Anacardiaceae. Pistacia vera, a congeneric species, had the highest sequence similarity with P. chinensis. (4) K-mer analysis showed that the genome size of P. chinensis was 418 Mb with a heterozygosity level of 1.89% and repetitive sequences ratio of 57.5%. The results revealed that the genome of P. chinensis was a complex genome with high heterozygosity and repetitive rates. It is recommended to combine PacBio sequencing technology and Hi-C sequencing in genome assembling with specific concern on heterozygous regions. This study provides valuable reference and important database for the genomics of P. chinensis.
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    Genome-wide Identification and Tissue Expression Analysis of the KNOX Gene Family in Altingia chinensis
    WANG An-bang, YE Xing-zhuang, ZHAO Jin-tao, CHEN Zhi-yun, WENG Hui-ying, LIN Mao, ZHANG Guo-fang
    Subtropical Plant Science    2025, 54 (1): 1-10.   DOI: 10.3969/j.issn.1009-7791.2025.01.001
    Abstract35)      PDF(pc) (2586KB)(209)       Save
    The KNOX gene family has important regulatory functions in leaf-shape growth and development in plants. This paper aims to analyze the KNOX gene family of Altingia chinensis and explore its tissue expression pattern. We used bioinformatics methods to identify and study AcKNOXs at the whole genome level of A. chinensis, and analyzed the gene location, gene structure, phyloevolutionary relationships, gene collinearity and the transcriptome expression. We identified 11 AcKNOXs from 8 chromosomes in the A. chinensis genome. Among them, AcKNOX6 and AcKNOX9 lack ELK and Homeobox KN domains, AcKNOX7 lacks ELK domain, the others have four conserved domains: KNOX 1, KNOX 2, ELK and Homeobox KN. 11 AcKNOXs are classified into Class Ⅰ (AcKNOX1, AcKNOX2, AcKNOX5, AcKNOX8, AcKNOX10, AcKNOX11), Class Ⅱ (AcKNOX3, AcKNOX4, AcKNOX7) and Class M (AcKNOX6, AcKNOX9). The AcKNOXs protein contains 144 to 444 amino acids. They were all located in the nucleus and are all acidic, unstable and hydrophilic. Through the expression pattern analysis, we found that AcKNOXs genes have tissue specificity. The majority of the members of the same subfamily showed the similar expression pattern. The main amplification mode of 11 AcKNOXs were segmental duplication, no tandem repeats were found. There were four pairs of genes with collinearity, AcKNOX3/AcKNOX4, KNOX2/AcKNOX8, AcKNOX6/AcKNOX9, and AcKNOX5/AcKNOX10, respectively. The results of this research provide the study of KNOX gene family with scientific basis, which help to do in-depth study on the genomic characteristics.
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    Study on the Spatial Distribution Pattern and Potential Distribution Area of National Key Protected Wild Plants in Shennongjia Forest Area
    CHEN Wei, LI Chu-ting, FU Qiang, LIU Xiu-qun
    Subtropical Plant Science    2025, 54 (1): 71-81.   DOI: 10.3969/j.issn.1009-7791.2025.01.009
    Abstract20)      PDF(pc) (3788KB)(200)       Save
    In this study, according to the Ministry of Ecology and Environment’s Technical Regulations for Investigation and Evaluation of Terrestrial Higher Plant Diversity in County Areas, the whole Shennongjia Forest Area was divided into 37 working grids (10 km × 10 km), and the spatial distribution pattern, protection status and potential distribution area of national key protected wild plants in the forest area were analyzed. The study found that: (1) There are 84 species (including varieties) of national key protected plants in Shennongjia Forest Area, including 7 species (including varieties) of national first-level key protected plants and 77 species (including varieties) of national second-level key protected plants. There are 79 species (including varieties) which have detailed distribution information. Among them, at the family level, Orchidaceae have the most species. At the genus level, Paris has the most species, and the life form of plants is mostly herbaceous. (2) In the analysis of horizontal distribution pattern, the number of national key protected plant species in grid 31 (Guanmen Mountain, Xiangxiyuan ) is the largest, which may be related to its location in the middle and high mountain areas with large fluctuations and in the Daba Mountains. (3) In the analysis of vertical distribution pattern, Middle-altitude forest belt ( 1200 m≤alt≤2300 m ) had the highest species richness, with a total of 67 species (including varieties) of national key protected plants, belonging to 45 genera in 29 families. (4) Screening out the hot spot area for 31 and 32 grids, both in Shennongjia National Park, indicating that the hot spot area is basically fully protected, and there is no protection vacancy in the hot spot area. There are 54 species (including varieties) of national key protected wild plants in Shennongjia National Park, with an in-situ protection rate of 64.29%. (5) Seven kinds of typical national key protected wild plants were screened out, and their current potential distribution areas were predicted. The potential distribution areas were mainly in the three areas of Hongping Town, Muyu Town and Dajiuhu Lake. Xinhua Town and Yangri Town also have some potential distribution areas, while there are basically no suitable areas in the northeast part of Shennongjia Forest Area. Among the seven national key protected wild plants, Taxus chinensis has the largest potential distribution area in Shennongjia Forest Area, accounting for 87.02% of the total area of Shennongjia Forest Area, and the area of high suitable area is 1562.50 km2.
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    Morphological Characteristics and Progress of Floral Bud Differentiation in Cerasus campanulata
    ZHU Xuan-yi, ZHANG Yang-ting, LI Wan-ying, LIN Wei-jie, ZHAO Kai, Zhou Yu-zhen
    Subtropical Plant Science    2024, 53 (6): 554-559.   DOI: 10.3969/j.issn.1009-7791.2024.06.008
    Abstract61)      PDF(pc) (9974KB)(185)       Save
    To investigate the floral bud differentiation process in different petal types of Cerasus campanulata, flower developments of three varieties were observed by paraffin sectioning method in this study, i.e. the single-petaled type Cerasus ×kanzakura ‘Praecox’, double-petaled type C. campanulata ‘Double-flowered’ and semi-double-petaled type C. kanzakura×campanulata ‘Satis in Pink’. The results demonstrated that all three varieties underwent six distinct stages of floral bud differentiation: initial differentiation period, inflorescence primordium differentiation period, sepal differentiation period, petal differentiation period, stamen differentiation period, and pistil differentiation period. During the petal and stamen differentiation stages, the three varieties showed significant regularity. The petal differentiation period progressively lengthened in the varieties ‘Praecox’, ‘Satis in Pink’, and ‘Double-flowered’, while the stamen differentiation period gradually shortened. It is believed that the formation of double flower of C. campanulata varieties mainly comes from stamen petal, which belongs to the origin of pistil and stamen. The semi-double-petaled ‘Satis in Pink’ had a floral bud differentiation cycle of 98 days, the single-petaled ‘Praecox’ took 105 days, and the double-petaled ‘Double-flowered’ required 112 days. The differentiation process in various floral forms of C. campanulata was largely similar, with stamen petalization contributing to the development of double flowers. This study provides a morphological basis for further investigation into the causes and molecular mechanisms underlying double flower formation in C. campanulata.
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    Differential Analysis of Volatile Oil Composition of Notopterygii Rhizoma et Radix from Different Origins and Habitats
    ZHANG Jun, LI Qian
    Subtropical Plant Science    2025, 54 (1): 11-21.   DOI: 10.3969/j.issn.1009-7791.2025.01.002
    Abstract25)      PDF(pc) (1648KB)(170)       Save
    The differences in volatile oil composition of Notopterygii Rhizoma et Radix (NRR) from different origins and habitats were studied to provide scientific basis for the quality control and resource development of NRR. Twenty batches of NRR herbs from different origins and habitats were used as experimental materials to extract the volatile oil by water vapour distillation. The volatile oil yields, compositions and relative contents of Notopterygium incisum (NI) and N. franchetii (NF) were systematically analyzed by GC-MS technology. The differences in chemical composition between NI and NF were interpreted from the perspective of volatile constituents by combining with cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Results showed that the average volatile oil content of NI was higher than that of NF, whereas NF had a richer variety of volatile oil constituents. A total of 206 constituents were identified in the volatile oils from 20 batches, which were mainly hydrocarbons and alcohols. There were only 3 common constituents in 20 batches of the samples, which were all hydrocarbons. In addition, the 5 constituents with the highest contents, namely, β-pinene, (+)-α-pinene, (+)-limonene, p-mentha-1,4-diene, (-)-terpinen-4-ol were also significantly different in the different origins and habitats samples. The chemometrics analysis showed that HCA, PCA and OPLS-DA could effectively distinguish NI from NF, but could not be differentiated between samples of different habitats. A total of 23 differentiated volatile oil constituents were screened out using VIP > 1 as a judgement criterion, which can be used as an important constituent for distinguishing NI from NF. There were significant differences in the types and contents of the volatile oils of NRR from different origins, but there were no obvious differences among different habitats, indicating that origins are the dominant factors among the many factors that cause differences in the volatile compositions of NRR. The results of this study provide a theoretical basis for the the quality evaluation system of NRR.
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    Flavonoid Biosynthetic Pathways of Bupleurum scorzonerifolium and B. chinense
    CHANG Pan, LI Qian
    Subtropical Plant Science    2025, 54 (2): 158-167.   DOI: 10.3969/j.issn.1009-7791.2025.02.005
    Abstract20)      PDF(pc) (2060KB)(160)       Save
    The differences between Bupleurum scorzonerifolium and B. chinense and their flavonoid biosynthesis pathway were studied by method of transcriptomics and metabolomics. Mass spectrometry imaging was used to investigate the in situ accumulation patterns of the major metabolites and intermediates of flavonoids. Results showed that the two species of Bupleuri Radix differed significantly, in which the flavonoid differential metabolites were regulated by differential genes and showed extreme variability, and 52 differential metabolites such as rutin, myricetin, sakuranetin and xanthohumol were screened, as well as differences in the pattern of in situ accumulation in the root cross-section, with the phloem of B. chinense being richer in flavonoid accumulation, and B. scorzonerifolium presenting a more homogeneous distribution profile. The mechanism of flavonoid biosynthesis in two species of Bupleuri Radix was revealed, and FLS, CHI and DFR genes were hypothesised to be the key genes in flavonoid biosynthesis. Meanwhile, analysis from the perspective of spatial distribution revealed that the accumulation of flavonoids in the root cross-section showed the characteristic of expanding outwards with the pith as the centre of the circle.
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    Advance on Investigation and Diagnostic Technology of Wood Decay Fungi in Urban Trees
    DENG Jia-ru, SUN Long-hua, ZHANG Jing-ai
    Subtropical Plant Science    2025, 54 (1): 100-108.   DOI: 10.3969/j.issn.1009-7791.2025.01.013
    Abstract15)      PDF(pc) (1670KB)(145)       Save
    Urban trees are an important part of urban green spaces and play important ecological functions. Wood decay fungi are one of the important threats to the health of urban trees. The occurrence of wood decay fungi in urban trees not only directly causes tree growth decline and affects the landscape, but also poses potential threats to personal and property safety due to tree decay and failure. Therefore, investigation and diagnosis of wood decay fungi have become an important part of tree risk assessment. This paper conducts a relatively systematic and comprehensive review from the aspects of domestic and foreign wood decay fungi resources investigation and diagnostic technology in urban trees. Wood decay fungi have been studied abroad for more than 200 years, but the main focus has been on research into the classification, decay mechanisms, and their role in tree risk assessment. Over the past 30 years, China has made considerable progress in research on wood decay fungi, with much of the focus on the diversity of large fungi in forest ecosystems and the systematic study of the taxonomic status of important fungal groups. Some advanced instruments such as tree radar and stress wave detection have been applied in diagnosing wood decay fungi, helping to assess the extent of decay and related risks and providing crucial technical support for the protection and management of urban trees. With the development of molecular biology, novel molecular detection techniques such as DNA barcoding, real-time PCR, and loop-mediated isothermal amplification have been widely applied, significantly improving the rapid diagnostic capabilities of wood decay fungi compared to traditional morphological identification methods. This paper aims to provide a reference for the identification and early diagnosis of wood decay fungi.
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    Identification and Expression Analysis of the TCP Gene Family in Liquidambar formosana
    ZHAO Jin-tao, YE Xing-zhuang, WANG An-bang, CHEN Zhi-yun, WENG Hui-ying, ZHANG Guo-fang
    Subtropical Plant Science    2025, 54 (2): 109-120.   DOI: 10.3969/j.issn.1009-7791.2025.02.001
    Abstract82)      PDF(pc) (3585KB)(139)       Save
    Liquidambar formosana, a representative deciduous species of the Altingiaceae, holds significant economic and ecological values. The TCP gene family plays a crucial role in plant growth, development, and adaptation to stress. In this study, we integrated the genomic and transcriptomic data of L. formosana and used bioinformatics approaches to identify the LfTCP gene family at the whole-genome level. We analyzed the physicochemical properties of the proteins, gene locations, phylogenetic and evolutionary relationships, intra- and interspecific collinearity, promoter cis-acting elements, and tissue-specific expression profiles. The LfTCP gene family consists of 22 members with protein lengths ranging from 197 to 552 amino acids. These 22 LfTCP genes are unevenly distributed across 12 chromosomes. Phylogenetic analysis indicates that there are 12 members in Class I and 10 members in Class II, including 7 members in the CIN subfamily and 3 members in the CYC/TB1 subfamily. The promoter cis-regulatory elements suggest that the LfTCP gene family may be involved in responses to light, hormones, and stress. Tissue-specific expression analysis shows that LfTCP genes exhibit distinct tissue expression patterns, with LfTCP6, LfTCP7, and LfTCP17 being specifically highly expressed in leaves. These genes can serve as important candidates for studying the growth and morphological development of L. formosana leaves. The results provide a reference for understanding the functions of the LfTCP gene family and the molecular mechanisms by which TCP regulates leaf growth and development in L. formosana.
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    Effects of Exogenous Gibberellin on Rice Seed Germination and Seedling Growth under Salt Stress
    QIAO Jiao
    Subtropical Plant Science    2024, 53 (6): 512-519.   DOI: 10.3969/j.issn.1009-7791.2024.06.003
    Abstract60)      PDF(pc) (475KB)(127)       Save
    This experiment investigated the effects of different concentrations of gibberellin (25, 50, 100 mg·L–1) on rice seed germination and seedling growth under sodium chloride stress. The results showed that during the seed germination stage, exogenous gibberellin enhances the germination of rice seeds under salt stress, with an increase in germination vigor, germination rate, and germination index, and a decrease in relative damage rate. During the growth stage of seedlings, morphological analysis showed that exogenous gibberellin alleviated the inhibition of salt stress on seedling growth, resulting in an increase in plant height, root length, root tip number, and leaf area; Physiological and biochemical analysis showed that exogenous gibberellin improved the activity of superoxide dismutase (SOD) in rice leaves under salt stress, reduced the content of malondialdehyde (MDA) in leaves, increased the content of photosynthetic pigments including chlorophyll a, b and carotenoids, enhanced photosynthesis, increased the effective quantum yield of PSⅡ [Y(Ⅱ)] and the photochemical quenching coefficient (qP), and decreased the non photochemical quenching coefficient (NPQ). In summary, exogenous gibberellin soaking treatment significantly promoted the germination of rice seeds under salt stress, the effects of sodium chloride stress on seedling growth and development was significantly reduced after spraying exogenous gibberellin, and the optimal concentration of gibberellin was 50 mg·L–1. The research results provide a theoretical basis for optimizing rice cultivation in saline soil.
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    Comparison of Salt Spray Tolerance Among 5 Varieties of Bougainvillea
    LIN Jia-zheng, CAO Jian-ting, WANG Zi-yi, ZHAN Fu-lin, LI Yan, GAN Si-quan, WANG Wen-qing
    Subtropical Plant Science    2025, 54 (2): 168-175.   DOI: 10.3969/j.issn.1009-7791.2025.02.006
    Abstract17)      PDF(pc) (1978KB)(104)       Save
    In order to screen suitable Bougainvillea varieties for coastal urban landscaping, salt spray resistant of five varieties commonly used in Xiamen were comprehensively evaluated. The seedlings of five Bougainvillea varieties, namely, B. glabra 'Mrs. Eva Mauve Variegata', B. buttiana 'Miss Manila', B. glabra 'Elizabeth Angus', B. glabra 'Magnifica' and B. glabra 'Alba' were used as experimental materials. The NaCl solution was sprayed with an ultrasonic humidification sprayer to simulate salt spray stress. The morphological changes of the plants were observed regularly, and the relative chlorophyll content, relative water content, superoxide dismutase activity (SOD), malondialdehyde (MDA), and proline content of the leaves were measured. The results showed that salt spray stress caused yellowing, scorching, and shedding of Bougainvillea leaves, and the severity increased with the increase of treatment time. 'Elizabeth Angus' and 'Magnifica' were the most affected, and the appearance of 'Mrs. Eva Mauve Variegata' was less affected. Compared with the control, the relative chlorophyll contents and relative water contents of each variety decreased significantly, with different degrees of decline. Under salt spray treatment, the MDA content of 'Mrs. Eva Mauve Variegata' and 'Elizabeth Angus' did not change significantly with the increase of time; compared with the control, the MDA content of 'Alba' showed a gradual downward trend, 'Elizabeth Angus' and 'Magnifica' gradually increased, while ‘Miss Manila’ first decreased and then increased, and 'Mrs. Eva Mauve Variegata' first increased and then decreased. Compared with the control, except for 'Elizabeth Angus' and 'Magnifica', the SOD activity of the other varieties increased to a certain extent; with the increase of time, the SOD activity of most varieties showed a trend of first increasing and then decreasing. The changing trend of proline content in each variety first increased and then decreased with the passage of time, and the increase in 'Alba' was the largest. The fuzzy mathematical membership function analysis showed that the salt spray resistance of the five varieties was ranked as follows: 'Alba'>'Mrs. Eva Mauve Variegata'> ‘Miss Manila’> 'Elizabeth Angus'>'Magnifica'.
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    Species Diversity of Lycophytes and Ferns in Huidong County, Guangdong Province
    HUANG Jun-kai, LI Xiao-ji, DONG Shi-yong
    Subtropical Plant Science    2025, 54 (2): 186-194.   DOI: 10.3969/j.issn.1009-7791.2025.02.008
    Abstract23)      PDF(pc) (2106KB)(80)       Save
    Huidong is a county of Guangdong Province in southern China, lying in the eastern region of the Guangdong-Hong Kong-Macao Greater Bay, and holds rich biodiversity. However, there is no checklist of lycophytes and ferns available for Huidong due to the lack of a comprehensive survey. Based on field surveys in recent years, the literature, herbarium specimens, and molecular phylogenetic analyses of some groups, we conducted crucial identifications of all specimens available to us from Huidong in order to document the species diversity and identify the floristic characteristics and rare and endangered species of lycophytes and ferns in this county. The main results include these five points: (1) New morphological forms were found in Selaginella heterostachys, Coryphopteris angulariloba, C. chingii, and Diplazium virescens. (2) Sphaerostephanos taiwanensis was confirmed with populations occurring in Guangdong, while Asplenium guangdongense was not supported to be an independent species from A. normale. (3) A population of Vandenboschia lofoushanensis, which had disappeared for nearly a century in the wild, was rediscovered in Huidong. (4) A total of 11 lycophyte species (belonging to 5 genera and 2 families) and 119 fern species (in 62 genera and 23 families) are recognized in Huidong. For ferns in this area, the Asia-Tropic distributing element and the East-Asian element are dominant, each accounting for about 36% species diversity, with relatively rich species in Polypodiaceae (17 spp.), Dryopteridaceae (15 spp.), and Thelypteridaceae (15 spp.). (5) Among lycophytes and ferns in Huidong, thirteen species are rare and/or endangered; eight species hold Class II national protection status in China, with Huperzia javanica and V. lofoushanensis accessed as endangered (EN) under IUCN criteria. A complete list of lycophytes and ferns in Huidong, along with the information on the distribution pattern for each taxon, is provided.
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    Determination of 10 Phenylethanol Glycosides in Cistanche deserticola from Different Origins and 2 Species of Host Plants Based on UPLC-QQQ-MS/MS
    ZHAO Fa-fa, GUO Ye-hong, GAO Pei-wen, CHEN Jin-hui, ZHANG Wen-yao
    Subtropical Plant Science    2024, 53 (5): 399-407.   DOI: 10.3969/j.issn.1009-7791.2024.05.002
    Abstract54)      PDF(pc) (947KB)(75)       Save
    Ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QQQ- MS/MS) was used for the simultaneous determination of 10 phenylethanol glycosides in Cistanche deserticola of 2 host species from different origins. The results showed that the linearity of the 10 components to be measured was good in their respective ranges (R2>0.9989), and the average recoveries of the controls were in the range from 94.61% to 102.71%, with the RSD values less than 3.39%, which indicated that the results of the established method were accurate. The total amount of phenylethanol glycosides of C. deserticola from Inner Mongolia was significantly higher than those of C. deserticola from Xinjiang and Gansu. The total amount of phenylethanol glycosides of Atriplex canescens – C. deserticola was in the order of Inner Mongolia>Xinjiang>Gansu, while the total amount of phenylethanol glycosides of Haloxylon ammodendron C. deserticola was in the order of Inner Mongolia>Gansu>Xinjiang. The total amount of phenylethanol glycosides of A. canescens – C. deserticola from the 3 origins was higher than those of H. ammodendron – C. deserticola. It provided a more convenient assay for the determination of phenylethanol glycosides among C. deserticola of different origins and different hosts, and also provided scientific ideas for the quality evaluation and host selection of C. deserticola.
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    Complete Chloroplast Genome Structural Characterization of Paludella squarrosa and Comparative Analysis with Its Alliance
    HUANG Ding-xu, MA Huai-fu, LIANG Sheng, HE Qin-qin, ZHANG Ting-yue, KONG Ling-xiong, LIU Bang-you, KANG Kai-li, PENG Tao
    Subtropical Plant Science    2025, 54 (2): 121-131.   DOI: 10.3969/j.issn.1009-7791.2025.02.002
    Abstract21)      PDF(pc) (4438KB)(73)       Save
    Paludella squarrosa is primarily distributed in wetland environments and is one of the key bryophyte species in wetland ecosystems, playing a unique role in wetland ecology and plant evolutionary studies. In this study, the chloroplast genome of P. squarrosa was assembled and annotated using Illumina next-generation sequencing technology, and its basic features were analyzed. The results showed that the chloroplast genome of P. squarrosa is 124 879 bp in size, containing a large single-copy region (LSC, 86 458 bp), a small single-copy region (SSC, 18 529 bp), and two inverted repeat regions (IRa and IRb, both 9946 bp), with a GC content of 28.9%. The genome contains 122 genes, including 77 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. Sequence analysis revealed 49 dispersed repeat sequences, predominantly palindromic repeats, and identified seventeentypes of simple sequence repeats (SSRs), with A/T repeats being the most common. Additionally, comparative genomic analysis with species of the order Splachnales from GenBank revealed high similarity between P. squarrosa and Tayloria sp. in genome size, gene content, and sequence composition. Phylogenetic analysis based on the rps4 and trnL-F genes indicated that P. squarrosa is closely related to Amblyodon dealbatus. As a representative species of Meesiaceae, the chloroplast genome study of P. squarrosa provides new insights into the evolutionary history, genomic characteristics, and ecological adaptation mechanisms of Meesiaceae plants.
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    Phenotypic Characteristics and Key Identification Points of Common Tabebuia and Handroanthus Species in Shenzhen
    ZHANG Xiao-feng, LUO Dong, YUAN Feng-jun, XU Gui-hong, TANG Jing-wen, LEI Jiang-li
    Subtropical Plant Science    2025, 54 (2): 195-200.   DOI: 10.3969/j.issn.1009-7791.2025.02.009
    Abstract24)      PDF(pc) (1109KB)(68)       Save
    Tabebuia and Handroanthus (Bignoniacaea) species are famous woody plants in the world, and are widely cultivated in Shenzhen. However, some species are phenotypically similar, making them difficult to distinguish. Additionally, there is considerable confusion in the use of their Chinese names and Latin names. The article investigated and described in detail the types, phenotypes, and phenological characteristics of Tabebuia and Handroanthus species cultivated in the urban greenland from Shenzhen. It also conducts comparative analyses by reviewing relevant data and literature, aiming to provide scientific basis for the identification of phenotypic traits and germplasm evaluation of Tabebuia and Handroanthus species. The results showed that among the urban greenland in Shenzhen, six species from two genera (Handroanthus and Tabebuia) were primarily cultivated. Among these, there were three species with red flowers: Handroanthus impetiginosus, H. heptaphyllus, and Tabebuia rosea; and three species with yellow flowers: H. chrysanthus, H. chrysotrichus, and T. aurea. Among these species, H. impetiginosus and H. heptaphyllus, H. chrysanthus and H. chrysotrichus are extremely similar in flower color. However, there are differences in terms of leaf serration, petiole characteristics, calyx shape, and pubescence, which can be used as distinguishing traits between the species.
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    Diversity Analysis of Phenotypic Traits of Cinnamomum burmannii from Different Provenances in China
    XU Ze-ning, LAN Cai-jia, LI Lu, CHEN Hua
    Subtropical Plant Science    2025, 54 (1): 54-61.   DOI: 10.3969/j.issn.1009-7791.2025.01.007
    Abstract17)      PDF(pc) (3385KB)(67)       Save
    This study investigated the phenotypic characteristics and diversity of Cinnamomum burmannii plants from various provenances across the country. Eight provenances were selected as test materials, and twenty-two biological phenotypic traits, including crown shape, leaf structure, branch formation, and plant height, were measured. Phenotypic variations were analyzed through correlation and difference analysis. The results indicated significant differences among the twenty-two biological phenotypic traits, particularly in plant height, ground diameter, petiole length, and leaf length. Notably, the provenances from Shaoguan and Jian'ou exhibited the highest growth increments, which aligns with their strong growth potential. Furthermore, the analysis of the primary phenotypic trait growth increments across different provenances revealed that the Gangzhou provenance showed the largest growth increment in leaf width, demonstrating significant variation. The Shaoguan provenance recorded the highest growth increment in leaf length, again with significant differences noted. Overall, the growth increment and potential of the Shaoguan provenance were found to be the most pronounced. Additionally, cluster analysis of C. burmannii from the eight provenances, based on biological phenotypic trait indicators, suggested that the similarity of phenotypic traits was somewhat correlated with geographical location. The findings of this research will provide quantitative baseline data on relevant phenotypic traits, contributing to the conservation and utilization of C. burmannii resources.
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    New Records of Seed Plants from Guangdong Province Ⅵ
    XIONG Qin-dai, XUE Jian-jun, LUO Wu-hong, ZENG Qiu-gen, WANG Shi-yang, LAI Xiao-chao, DENG Jun, KANG Ning, FAN Qiang
    Subtropical Plant Science    2025, 54 (2): 209-216.   DOI: 10.3969/j.issn.1009-7791.2025.02.011
    Abstract35)      PDF(pc) (2327KB)(56)       Save
    Six new records of seed plants in Guangdong Province are reported, namely Pinus fenzeliana Hand.-Mazz., Stemona japonica (Bl.) Miq., Scutellaria yunnanensis H. Lév., Ehretia densiflora F. N. Wei & H. Q. Wen, Elatostema pycnodontum W. T. Wang and Rubus jianensis L. T. Lu &Boufford. These new records enrich the plant diversity in Guangdong Province, which are of great significance for the study of the flora of seed plants in Guangdong Province.
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    Effects of Drought Stress on Root Morphological in Seedlings of Five Species of Garden Plants
    CHEN Xiao-xi, ZHANG Yan-jing, LAI Can, WU Jia, TAN Can-can, GUO Wei, ZHAN Guo-qiang, SUN Yan-jun
    Subtropical Plant Science    2024, 53 (6): 520-526.   DOI: 10.3969/j.issn.1009-7791.2024.06.004
    Abstract65)      PDF(pc) (2269KB)(55)       Save
    This study investigated the effects of drought stress on the root morphological of urban trees. Two-years-old seedlings of Delonix regia, Spathodea campanulata, Camphora officinarum, Terminalia neotaliala and Ceiba speciosa were used as research subjects. And the changes in root morphology were compared across the different levels of drought stress. The results showed that with the increase of drought stress intensity, the total root length and average connection length of C. officinarum under CK treatment were significantly reduced compared to M and S treatment, and the branch density was significantly increased (P<0.05); The average connection length of T. neotaliala significantly decreased, and the branch density significantly increased. Under drought stress, most root architecture indicators showed a highly significant correlation (P<0.01). As drought stress increased, the correlations among root traits also intensified, particularly under severe drought conditions, where a strong correlation was observed between total root length and the number of root forks. This indicated that under drought stress, tree resource allocation patterns may change significantly, affecting the interrelationships among various root traits. Using principal component analysis (PCA) and maximum variance rotation, the primary structural indicators identified were the number of root forks, total root length, and root crossings. The comprehensive scores of the species in response to drought stress were ranked in descending order as follows: Terminalia neotalialaDelonix regiaCamphora officinarumSpathodea campanulataCeiba speciosa.
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    Evaluation of Cold Resistance of 9 Clones of Acacia mangium × A. auriculiformis
    LIAO Fang-yan, CHEN Bo-zhen, WANG Li-han, WANG Yu-xin, LI Na, ZHU Bao-zhu, ZHANG Wei-hua
    Subtropical Plant Science    2025, 54 (1): 45-53.   DOI: 10.3969/j.issn.1009-7791.2025.01.006
    Abstract33)      PDF(pc) (638KB)(54)       Save
    The seedlings of 9 clones of Acacia mangium × A. auriculiformis  were used as experimental materials, and the cold environment was simulated by a large low-temperature artificial climate chamber. Physiological indexes such as relative electrical conductivity (REC), soluble protein (SP), free proline (Pro) and malondialdehyde (MDA) were determined, and the response of physiological indexes, cold resistance were analyzed under low temperature conditions, and the cold resistance of 9 clones of A. mangium × A. auriculiformis were evaluated by membership function method comprehensively. Results showed that the relative electrical conductivity and soluble protein content of leaves of different clones increased gradually, the free proline content and SOD activity increased first and then decreased, and the MDA content increased first and then decreased and then increased under low temperature stress. According to the average membership function value, the rank of the cold resistance of 9 clones of A. mangium×A. auriculiformis is No. 8 > No. 7 > No. 6 > No. 2 > No. 1 > No. 9 > No. 3 > No. 4 > No. 5. The No. 8 has the strongest cold resistance, and it can be used as a high quality cold resistance material for hybrid breeding and cultivation.
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    Recovery Characteristics of Species Diversity and Community Structure in Subtropical Mixed Plantations after 15 Years of Natural  Succession
    PAN Li-jun, Du Jian, YE Xiao-ping, XU Han, LI Yan-peng, CHEN Zhao-cong
    Subtropical Plant Science    2025, 54 (1): 62-70.   DOI: 10.3969/j.issn.1009-7791.2025.01.008
    Abstract14)      PDF(pc) (502KB)(53)       Save
    The resilience of species diversity and community structure are important indicators to judge the success of mixed plantation construction. This study was conducted in the subtropical mixed plantations formed after the renovation of pure forest of Cunninghamia lanceolata in 2005 in Yunyong forest farm, Foshan City, Guangdong Province. Recovery characteristics of species diversity and community structure were analyzed based on the 6.48 hm2 plot which was set up in the typical areas of the above plantations in 2020. The results were as follows: (1) 169 woody plant species belonging to 121 genera in 55 families colonized the community after 15 years of natural restoration, indicating the subtropical mixed plantations had a strong conservation ability of species diversity. (2) Seven of the top ten species in terms of importance value were artificially introduced species, indicating that early introduced species still had an important effect on the community structure of subtropical mixed plantations after 15 years of natural succession. (3) The average diameter at breast height (DBH) of all individuals was 8.60 cm, and the DBH distribution was an inverse-J shape, indicating the community had normal regeneration ability. (4) Early artificially introduced species could also have normal regeneration ability, although their DBH shapes mainly showed a bell curve due to the short recovery time. The results revealed the subtropical mixed plantations had strong conservation ability of species diversity and community structure. These findings could provide a theoretical basis for optimizing forest restoration strategies in subtropical areas.
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    Auxin Signaling Pathway-Mediated Regulation of Lateral Root Formation of Maize Plants by Arbuscular Mycorrhizal Fungus
    ZHU Feng-wa, CHEN Wei-li, ZHANG Wei, CHEN Mei-zheng, LIU Xiao-di, YAO Qing
    Subtropical Plant Science    2025, 54 (2): 132-143.   DOI: 10.3969/j.issn.1009-7791.2025.02.003
    Abstract45)      PDF(pc) (3238KB)(45)       Save
    Arbuscular mycorrhizal (AM) fungi have the capacity to alter plant lateral root (LR) formation, a process that is highly dependent on phosphorus (P) levels and plant hormones. This study using maize (Zea mays) as test plants, Rhizophagus irregularis was inoculated in soils with no P application (soil available P of 20.5 mg·kg–1, namely low P level) and additional P application of 30 mg P·kg–1 as KH2PO4 (namely high P level). Plant biomass, LR number, the contents of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in roots were determined, and the expression of LR formation-related genes (ZmKRP2, ZmPSK1, and ZmPSK3) and auxin signaling-related genes (ZmNAC1, ZmARF1, ZmTIR1, ZmPIN1b, and ZmAUX1) was also quantified. At 35 days after sowing, AM fungal significantly increased maize biomass at both phosphorus levels compared with no inoculation. AM fungal inoculation significantly increased the numbers of 2nd LR and 3rd LR by 53.5%–237.8%, while the effect of P levels on LR number was relatively weak. AM fungal inoculation significantly enhanced the expression of ZmPSK3, and P levels enhanced the expression of ZmPSK1, ZmPSK3, and ZmKRP2. Additionally, IAA contents and signaling pathways were significantly affected by both P levels and AM fungal inoculation. When inoculated with AM fungus, IAA content at 35 days after sowing at low P level was significantly increased by 48.4% than that at high P level. AM fungal inoculation significantly upregulated the expression of auxin signaling-related genes. Compared with 1ow P level, high P level significantly increased the expression of ZmNAC1, but decreased the expression of ZmAUX1. The findings indicated that ZR content was not affected by P level and AM fungus, while the auxin signaling pathway was involved in the LR formation mediated by AM fungal inoculation, whose effect magnitude was more pronounced that that of P levels.
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    Current Status of Molecular Markers and Genomics of Anoectochilus Herba
    LIN Shu-qing, MA Xiang-li, XU Wen, HUANG Ze-hao
    Subtropical Plant Science    2024, 53 (5): 478-486.   DOI: 10.3969/j.issn.1009-7791.2024.05.013
    Abstract109)      PDF(pc) (442KB)(35)       Save
    With the development of molecular biotechnology, significant progress has been made in the molecular pharmacognosy research of Anoectochilus herba. This paper summarized the molecular pharmacognostic research on molecular identification, transcriptome sequencing, functional gene cloning and synthesis of Anoectochilus herba, and discussed the problems and prospects of molecular marker biotechnology in the genetic analysis, molecular identification and functional gene discovery, in order to further promote the protection, development and utilization of Anoectochilus herba resources.
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    Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High Temperature and Drought Stress
    ZHOU Hong-hui, GE Cong, HUANG Xiao-bing, ZHANG Long, LU Cong-hui, ZHANG Yu-ping, WANG Ping
    Subtropical Plant Science    2024, 53 (5): 389-398.   DOI: 10.3969/j.issn.1009-7791.2024.05.001
    Abstract98)      PDF(pc) (1709KB)(34)       Save
    The effects of inoculation with Arbuscular mycorrhizal fungi (AMF) on the photosynthesis and antioxidant capacity in the leaves of Citrus reticulata ‘Ponkan’ under high temperature and drought stress were investigated. This study would provide Ponkan a production measure for resisting high temperature and drought stress. The experimental treatments were designed as follows: (1) Ponkan without AMF inoculation under high temperature and drought stress; (2) Ponkan with AMF inoculation under high temperature and drought stress; (3) Ponkan without AMF treatment under normal environmental conditions (control). In this study, AMF infection rate in the root and phenotype of Ponkan were observed, and the photosynthetic parameters, reactive oxygen species (ROS) content and antioxidant enzyme activities were measured in the leaves. The results showed that the leaves of Ponkan without AMF inoculation were obviously yellowed and wilted under high temperature and drought stress compared with the control. The net photosynthetic rate, stomatal conductance, transpiration rate, water use efficiency, maximum photochemical efficiency, actual photosynthetic efficiency and photosynthetic electron transport efficiency in the leaves of Ponkan without AMF inoculation were significantly decreased under high temperature and drought stress, while the intercellular CO2 concentration was increased compared with those of control. However, the net photosynthetic rate and stomatal conductance were increased, the intercellular CO2 concentration was decreased, and the photosynthetic capacity was enhanced in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. In addition, the contents of O2܋, H2O2 and malondialdehyde (MDA) in leaves of Ponkan without AMF inoculation were increased while the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) were decreased under high temperature and drought stress compared with those of control. However, the contents of O2܋, H2O2 and MDA were decreased and the activities of the antioxidant enzymes were increased in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. The results demonstrated that Ponkan growth was inhibited, and the photosynthetic and antioxidant capacities was reduced in the the leaves of Ponkan without AMF inoculation, while the capacities were effectively improved in the leaves of Ponkan with AMF inoculation under high temperature and drought stress. Therefore, we conclude that the higher photosynthetic and ROS scavenging capacities in the leaves of Ponkan with AMF inoculation alleviate the physiological damage of the plants under high temperature and drought stress.
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    Effects of Salicylic Acid Treatment on Physiology Quality of Postharvest Longan Fruit
    WANG Hui-ling, SUN Jun-zheng, LIU Qing-qing, MING Yan-lin, LIN He-tong, CHEN Yi-hui
    Subtropical Plant Science    2024, 53 (6): 527-536.   DOI: 10.3969/j.issn.1009-7791.2024.06.005
    Abstract57)      PDF(pc) (802KB)(34)       Save
    Longan (Dimocarpus longan) fruit has high nutritional value. However, the fruit matures during the season of high temperature and high humidity, from July to September. The postharvest physiological metabolism of longan fruit is vigorous. After 3 or 4 days of storage at room temperature, the endocarp exhibited browning, and the pulp was breakdown, which finally caused deterioration. In this study, ‘Fuyan’ longan fruit was used as material to evaluate the effects of salicylic acid (SA) treatment on postharvest physiological quality of Phomopsis longanae-inoculated longan fruit stored at 28 ℃. The results showed that SA treatment decreased the indices of pericarp browning, pulp breakdown, and fruit weight loss percentage. On the 5th day of storage, the pericarp browning index, pulp breakdown index, and fruit weight loss rate in the treatment group of SA+P. longanae were 7.65%, 15.91%, and 24.57% lower than those of the control group of P. longanae. Meanwhile, SA treatment delayed the decrease in L* value, and contents of chlorophyll, carotenoid, anthocyanin, flavonoids, and total phenolic in the pericarp, maintained higher contents of total sugar, sucrose, total soluble solids (TSS) and vitamin C in the pulp of longan fruit, and delayed the increase of titratable acids (TA) and reducing sugar contents in longan fruit. This study proved that SA treatment could maintain better physiological quality and improve the storability of harvested longan fruit.
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    Pathogenicity of Corynespora cassiicola in Different Tobacco Cultivars and Optimization of Culture Conditions
    HUANG Chun-yang, YANG Xiang, ZHOU Hong-jiang, SHI Cai-hua, WANG Han-cheng, HU Jing-rong
    Subtropical Plant Science    2025, 54 (1): 22-28.   DOI: 10.3969/j.issn.1009-7791.2025.01.003
    Abstract16)      PDF(pc) (1264KB)(32)       Save
    In order to elucidate the pathogenicity of Corynespora cassiicola and determine its optimization of cultivation conditions, this study conducted inoculation experiments using six common tobacco varieties inoculating with C. cassiicola strain HBU230811. The findings revealed varying levels of infection across all tested varieties; notably, Guizhou No.5 exhibited pronounced resistance against this strain. The ability for growth was observed across multiple culture media including PDA, MS, OA, KBA, LB, and TLA; although there were no discernible differences in colony diameter between KBA and OA, the size remained significantly larger compared to other media. On OA, mycelium appeared thinner whereas it displayed denser characteristics when grown on KBA, resulting in shorter spore-forming times. Moreover, the strain demonstrated normal growth within a pH range from 4 to 12, yet optimal conditions were observed at a pH level between 7 and 9. Additionally, various factors such as sugar sources, duration of light exposure, and temperature exerted significantly influences on the growth of C. cassiicola strain HBU230811. Notably, sorbitol as a sugar source combined with cultivation at 28 ℃ under complete darkness yielded the most favorable growth conditions. These findings contribute to establishing a theoretical foundation for understanding the pathogenic ecological characteristics of C. cassiicola strain HBU230811.
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    Ultrasound-Assisted Deep Eutectic Solvent Extraction of Gymnema sylvestre Polysaccharides and Its Activity Determination
    HAO Sheng-jie, HOU Hai-qing, YIN Jie, LI Hong-sen, DU Yu-xin, JIN Feng-yang, LI Qian, CHEN Gui-lin
    Subtropical Plant Science    2024, 53 (6): 545-553.   DOI: 10.3969/j.issn.1009-7791.2024.06.007
    Abstract51)      PDF(pc) (3283KB)(30)       Save
    Optimization of ultrasonic-assisted deep eutectic solvents (DESs) extraction process for Gymnema sylvestre polysaccharides was conducted to investigate its biological activity, to provide theoretical support for the development of G. sylvestre products. Using the polysaccharide extraction yield as the evaluation index, the Box-Behnken response surface method was applied to optimize the extraction process based on single-factor experiments. Additionally, the antioxidant activities of the polysaccharides were evaluated by their ability to scavenge DPPH, ABTS+, FRAP, and H2O2 radicals, while the inhibitory effects on α-amylase and α-glucosidase, as well as antibacterial activity against several bacteria strains, were also studied. The results showed that the DESs synthesized from choline chloride and citric acid achieved the highest extraction rate. After optimization using the response surface method, under conditions where the molar ratio of choline chloride to citric acid was 1:1.02, water content was 39.32%, liquid-to-material ratio was 20.21:1, ultrasonic time was 39.23 min, ultrasonic temperature was 41.35 ℃, and ultrasonic power was 412 W, the extraction yield of G. sylvestre polysaccharides reached 29.23%, which was nearly 15 times higher than the traditional water extraction method. In vitro activity experiments demonstrated that G. sylvestre polysaccharides exhibited high antioxidant activity, broad-spectrum antibacterial capacity, and enzyme inhibition activity, showing great potential for developing novel anti-diabetic products. DESs, being environmentally friendly, efficient, and reusable as extraction media for natural products, significantly improved the extraction efficiency of G. sylvestre polysaccharides.
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    Cheirostylis sherriffii, a Newly Recorded Species of Orchidaceae from China
    FU Xiao-ying, XIONG Chi
    Subtropical Plant Science    2024, 53 (5): 471-473.   DOI: 10.3969/j.issn.1009-7791.2024.05.011
    Abstract60)      PDF(pc) (1076KB)(30)       Save
    Cheirostylis sherriffii N. Pearce & P. J. Cribb was reported as a newly recorded species of Orchidaceae from China. The report included characteristic descriptions and color photos. This species was similar to C. glandulifera (Aver.) J. M. H. Shaw, but could be distinguished by its erect stems, short rhizome, 3-6 flowered inflorescence, and pale green sepals and petals. Voucher specimens were deposited in the Herbarium of Guangxi Institute of Botany (IBK).
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    Moliniopsis, a Newly Recorded Genus of Poaceae in Guangdong Province
    TONG Yi-hua, ZENG Yun-bao
    Subtropical Plant Science    2025, 54 (1): 85-88.   DOI: 10.3969/j.issn.1009-7791.2025.01.011
    Abstract25)      PDF(pc) (5695KB)(28)       Save
    This paper reported a newly recorded genus of Poaceae in Guangdong, viz. Moliniopsis Hayata. This genus is monotypic with the type species, M. japonica (Hackel) Hayata, distributed in Russian Far East, Japan, South Korea and Southeast China (Anhui, Zhejiang, Jiangxi, Fujian). Recently, a population of this species was found in Conghua District, Guangzhou City, Guangdong Province, which is the southernmost population of this species. This new finding is of great significance for the future study of the geographical distribution and the origin and the spread of M. japonica. The vouchers are deposited in the herbarium of South China Botanical Garden, Chinese Academy of Sciences (IBSC).
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    New Records of Seed Plants in Xizang, China
    XI Hou-cheng, SHEN Jian-yong, JIANG Li-ju, MA Xing-da, WANG Wen-guang
    Subtropical Plant Science    2025, 54 (1): 82-84.   DOI: 10.3969/j.issn.1009-7791.2025.01.010
    Abstract14)      PDF(pc) (1796KB)(27)       Save
    Amomum raoii var. oblongum, a new recorded variety from China and Phlogacanthus gomezii, a new record species of Xizang found in Medog County, Xizang, are reported in this paper. Supplementary descriptions of morphological characters are provided based on the protologues and voucher specimens. The vouchers are kept in the herbaria of Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences (HITBC) and Fairy Lake Botanical Garden, Shenzhen and Chinese Academy of Sciences (SZG).
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    New Records and New Form of Spermatophyte Distributed in Anhui Province
    SUN Xiang-yang, SHEN Lang, ZHU Hao, MA Hai-jun, NI Wei-yong, FANG Hong-ming, LI Xiao-hong, SHAO Jian-wen, LIU Kun
    Subtropical Plant Science    2024, 53 (5): 474-477.   DOI: 10.3969/j.issn.1009-7791.2024.05.012
    Abstract74)      PDF(pc) (1749KB)(27)       Save
    Based on field survey and specimen examination, three species and one form are reported as new records in Anhui Province, viz. Elsholtzia argyi Lévl f. albiflora X. Y. Sun & Kun Liu , Euonymus dielsianus Loes. ex Diels, Goodyera velutina Maxim, Ardisia alyxiifolia Tsiang ex C. Chen, in which, Elsholtzia argyi f. albiflora is a new form. This paper briefly describes the distribution, habitat, major identifying features, and differences from similar species for each species with color photos.
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    Diversity of Floral Traits and Ecological Strategies in 20 Apocynaceae Plants
    SUN Rui, SONG Peng-fei, YANG Shuai, HAN Mei, YANG Jie
    Subtropical Plant Science    2024, 53 (5): 433-443.   DOI: 10.3969/j.issn.1009-7791.2024.05.006
    Abstract80)      PDF(pc) (1509KB)(23)       Save
    Floral traits, as one of the most diverse and complex collections of traits in life history stages of plants, perform ecological functions such as attraction, defence and resistance to achieve successful reproduction. Flower colour has lagged behind other traits in the study of floral traits, but flower color and its relationship with other floral traits play an important role in attracting pollinators and reproducing offspring in plants. In this study, we analyzed the diversity and correlation of five types of floral traits, including flower color, in 20 species of Apocynaceae, aiming to gain a deep understanding of the diversity of floral traits and their ecological strategies. The results showed that there was a wide diversity of floral traits in Apocynaceae, with significant differences between some floral traits of plants with different flower colors. In addition, we found different degrees of correlation between different floral traits in Apocynaceae, suggesting that plants are able to adopt appropriate ecological strategies through their own needs.
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    Root Structure Characteristics of Phyllostachys edulis Forests and P. praecox Forests
    LÜ Hui-fei, WANG Ling-ling, ZHAO Zi-qing, SUN Han-jing, ZHENG Hui
    Subtropical Plant Science    2024, 53 (5): 451-458.   DOI: 10.3969/j.issn.1009-7791.2024.05.008
    Abstract48)      PDF(pc) (548KB)(23)       Save
    The study of differences in root structure characteristics between Phyllostachys edulis and P. praecox forests through measurements of length, volume, surface area, and dry weight of the root was carried out, which provided a basis for bamboo resource cultivation, soil management and enhancing the economic benefits of bamboo forests. The soil distribution characteristics of bamboo forest roots were investigated by using the methods of root digging scanning. P. edulis roots mainly distributed in the soil layer of 0-40 cm, and the root surface area, volume, length and dry mass accounted for more than 80% of the total root system. The proportions of root length and volume of different diameter classes in P. edulis and P. praecox forests showed the decreasing trend with the deepening of the soil layer. In the different diameter classes of P. edulis roots, roots with diameters<1 mm had the highest proportion of length (70.45%), whereas those with diameters>2 mm had the highest proportion of volume (78.73%). In P. praecox forest, the roots were distributed only in the soil layer of 0-40 cm, and the root volume, surface area, and dry weight accounted for more than 60% in the surface soil layer (0-20 cm). The root length accounted for 59.39% of the total length, and roots with diameters<1 mm had the highest proportion of length (80.46%) among different diameter classes, though they had the smallest proportion of volume (16.63%). In the soil layer of 0-40 cm, the proportion of root length in three diameter classes showed that P. praecox forest was greater than P. edulis forest. The proportion of roots with diameters≤2 mm showed that P. edulis forest was less than P. praecox forest. The proportion of roots with diameters>2 mm in P. edulis forest was greater than P. praecox forest. The soil layer of 0-40 cm is the main active layer for the growth of bamboo forest roots, and it is also the important level for soil management in bamboo forests. P. edulis and P. praecox differ significantly in root content and structure. P. edulis has the larger root structure compared to P. praecox. Therefore, it is necessary to propose measures that are more suitable for bamboo management by combining different root structure characteristics.
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    Effects of Water and Nitrogen Coupling on Yield of Astragalus membranaceus and Soil Moisture, Water Consumption Characteristics Under Mulched Drip Irrigation
    CUI Xiong-fei, WANG Yu-cai, LÜ Xiang, WANG Xiao-feng
    Subtropical Plant Science    2025, 54 (2): 176-185.   DOI: 10.3969/j.issn.1009-7791.2025.02.007
    Abstract16)      PDF(pc) (2164KB)(23)       Save
    To clarify the effects of water-nitrogen coupling on soil moisture changes, water consumption characteristics, and yield of Astragalus membranaceus in the semi-arid region of Longxi, and to explore irrigation and fertilization patterns of efficient and reasonable suitable for local A. membranaceus, a water-nitrogen coupling experiment using drip irrigation under plastic film was conducted in Weiyuan County, Gansu Province, from April to October 2024.A two-factor experimental design was adopted, with soil moisture content set at two irrigation levels: 55%–70% of field capacity (W1) and 70%–85% of field capacity (W2), and three nitrogen levels: low nitrogen (62 kg·hm–2, N1), medium nitrogen (84 kg·hm–2, N2), and high nitrogen (106 kg·hm–2, N3).The results showed that the soil water storage capacity at depths of 0–100 cm decreased as the astragalus growth period progressed. Appropriate increases in nitrogen fertilizer application reduced soil water consumption. The total water consumption, stage-specific water consumption, water consumption intensity, and water consumption coefficient were highest during the fruiting period of A. membranaceus. Among these, the W2N1 treatment had the highest total water consumption, reaching 674.3 mm; the W2N2 treatment had the highest water consumption and water consumption intensity, which were significantly higher than other water-nitrogen treatments by 3.0%–16.8% and 3.4%–17.9%, respectively, and significantly higher than the CK treatment by 39.8% and 39.4%, respectively. The W1N2 treatment had the highest water use coefficient, significantly higher than other water-nitrogen treatments by 5.4% to 17.3%, and significantly higher than the CK treatment by 2.1%. Different water and nitrogen treatments had a significant effect on the root diameter, root length, underground dry weight, and yield of A. membranaceus, with the W2N2 treatment yielding the highest results. Compared to other treatments, the W2N2 treatment significantly increased root diameter by 9.8% to 48.3%, root length by 5.6% to 22.4%, underground dry weight by 14.2% to 51.3%, and yield by 7.1% to 25.9%. This indicates that appropriate irrigation and nitrogen application can improve A. membranaceus' water use characteristics, promote root system absorption of soil moisture, and enhance water use efficiency. The W2N2 treatment cultivation model significantly increases root diameter, root length, and A. membranaceus yield.
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    Development Status of IWGS International New Waterlily Competition and Characteristics of the Awarded Waterlily Cultivars
    LIU A-mei, LI Zi-jun, TIAN Dai-ke
    Subtropical Plant Science    2025, 54 (2): 220-228.   DOI: 10.3969/j.issn.1009-7791.2025.02.013
    Abstract17)      PDF(pc) (3963KB)(22)       Save
    The International Waterlily & Water Gardening Society (IWGS) began to hold the International New Waterlily Competition every year since 1997. The competition has been held 25 times so far, and 162 cultivars from 61 breeders (company) of 10 countries have been awarded. For a comprehensive understanding of the characteristics of the competition and the awarded waterlilies, and the development trend of this competition and waterlily breeding, and for improving breeding activities and participation of the competition, this paper systematically summarized the history, rules and development status of the waterlily competition, the distribution of the breeding countries, the characteristics of the awarded cultivars, the main participating countries and breeders.
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    Full-length Transcriptome Sequencing Analysis of Medicinal Plant Pithecellobium clypearia
    ZHONG Feng-di, LING Teng-hong, MOU Gui-ping, ZHU Kong-yu, WANG Peng-long, YAN Xiao-dong
    Subtropical Plant Science    2024, 53 (5): 416-424.   DOI: 10.3969/j.issn.1009-7791.2024.05.004
    Abstract45)      PDF(pc) (1099KB)(22)       Save
    As the plant with remarkable medicinal value, the insufficiency of genomic information of Pithecellobium clypearia restricts its in-depth comprehension. In this study, PacBio Sequel sequencing technology was employed to conduct full-length transcriptome sequencing of the mixed samples from roots, mature branches, young branches, mature leaves, and young leaf tissues of P. clypearia. The results showed that 118 472 unique transcripts were successfully identified and further categorized into 44 939 Unigenes. After comparison with the Nr, Uniprot, GO, KEGG, and KOG databases, functional annotations were provided for 37 198 Unigenes, and the majority of these Unigenes were involved in biological processes and metabolic pathways. Simultaneously, 13 897 Unigenes were discovered to undergo alternative splicing, including intron retention, 3' end splicing diversity, and 5' end splicing diversity. Additionally, 377 and 159 structural genes involved in the biosynthesis of flavonoids and terpenoids, as well as 132 and 180 Unigenes encoding members of the MYB and WRKY transcription factor families, were identified. This study expanded the genomic resources of P. clypearia, provided valuable data support for exploring the metabolic regulatory mechanism of its bioactive components, screening of key genes, and development of molecular markers, laid a foundation for further exploitation of the medicinal value of P. clypearia in future.
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    Genome Size Estimation of Uraria lagopodioides and Christia vespertilionis (Fabaceae) by Flow Cytometry
    GAO Ying-jie, MIAO Jia, HUANG Xiang, ZHAO Xue-li
    Subtropical Plant Science    2024, 53 (6): 503-511.   DOI: 10.3969/j.issn.1009-7791.2024.06.002
    Abstract47)      PDF(pc) (808KB)(22)       Save
    This study aimed to determine the genome size of medicinal plants Uraria lagopodioides and Christia vespertilionis (Fabaceae), and to explore the appropriate experimental conditions. We screened five nuclear dissociation buffer and two methods for preparing nuclear suspensions, and then we estimated the genome size of both species by flow cytometry using the most appropriate experimental conditions with Zea mays as an internal standard plant. The results revealed that MGB was a suitable nuclear dissociation buffer for flow cytometry of both plants, and filtering through the 40 μm mesh for three times or centrifugation at 1000 r·min–1 was the appropriate method for preparing nuclear suspensions. The genome size of U. lagopodioides and C. vespertilionis were estimated to be 0.69 Gb and 0.64 Gb, respectively. Genome size estimation and the exploration of suitable experimental conditions for flow cytometry in this study can provide reference for the genomics, genetic evolution, and resource development of U. lagopodioides and C. vespertilionis.
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    Comprehensive Evaluation on the Ornamental Value of 13 Paeonia lactiflora Varieties Based on Analytic Hierarchy Process
    YING Meng-yun, SHI Jian-yu, YE Qiu-ping, SHEN Qing-yu, YU Wen
    Subtropical Plant Science    2024, 53 (6): 577-583.   DOI: 10.3969/j.issn.1009-7791.2024.06.011
    Abstract52)      PDF(pc) (496KB)(22)       Save
    Thirteen high-quality ornamental varieties of Paeonia lactiflora were collected and observed in this study. Selecting indicators based on a comprehensive evaluation model for the ornamental value of varieties. Thirteen ornamental indicators based on flower traits, plant traits, and growth properties were selected to comprehensively evaluate the ornamental values of P. lactiflora varieties using analytic hierarchy process (AHP) method. The results showed that according to the constructed judgment matrix, the weight of flower color, plant type, and growth potential in the indicator layer were relatively high, which was particularly important in the evaluation of the ornamental value of P. lactiflora. These varieties were divided into gradeⅠ,Ⅱand Ⅲ based on the comprehensive scores. The four varieties of gradeⅠhad the best ornamental effects, including ‘Xueyuanhongxing’, ‘Fengyuluojinchi’, ‘Qihualushuang’ , ‘Zhongshengfen’ , which had good adaptability and ornamental value. They were advisable to leverage their advantages for promotion. The comprehensive performance of the six varieties of grade Ⅱ was average, including ‘Moziling’, ‘Dafugui’, ‘Qingwen’, ‘Lanfushi’, ‘Luhong’, ‘Yanzixiangyang’. The comprehensive evaluation value of grade Ⅲ was relatively low, including ‘Hongfushi’, ‘Hongyanzhenghui’, ‘Zifengzhaoyang’. The comprehensive evaluation results of the ornamental value of P. lactiflora based on the analytic hierarchy process could provide reference for the application of P. lactiflora resources in ornamental horticulture.
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    Analysis of the Current Status and Spatial Distribution of Ancient and Famous Trees in Xiamen City
    WANG Long-ping
    Subtropical Plant Science    2024, 53 (6): 569-576.   DOI: 10.3969/j.issn.1009-7791.2024.06.010
    Abstract46)      PDF(pc) (2254KB)(21)       Save
    This study analyzed the current status and spatial distribution characteristics of ancient and famous trees in Xiamen City, with the aim of gaining a deeper understanding of the situation of these resources and providing a scientific basis for their protection and management. Based on data from the 2021–2023 survey of ancient and famous trees in Xiamen, the study employed a combination of GIS information technology and traditional analysis methods to examine three diversity indices of ancient and famous trees, as well as their distribution in relation to administrative regions, topography, slope, and aspect. Xiamen City has a total of 1773 ancient and famous trees registered in its records,including 11 famous trees, 68 first-grade ancient trees, 319 second-grade ancient trees, and 1375 third-grade ancient trees, belonging to 43 species of 39 genera in 27 families. The proportion of those with normal growth potential is 82.18%. The species are predominantly native, with banyan trees being the most numerous. The Shannon-Wiener (H) index for biodiversity is 1.588, the Pielou evenness index is 0.420, and the Margalef richness index is 5.882, indicating a moderate level of biodiversity. The nearest neighbor ratio for ancient and famous trees in Xiamen City is 0.30, which is less than 1, indicating a clustered spatial distribution type. The highest density is found in Gulangyu, Siming District, exceeding 26.9 trees per square kilometer. 94.6% of these trees are located on slopes less than 15 degrees, and they are evenly distributed across various aspects. Among different land use types, residential and construction land has the highest proportion of ancient and famous trees, accounting for 63.8%, followed by farmland at 29.9%.
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    Morphological Supplement to Parahellenia malipoensis (Costaceae)
    YAN Jia-wei, CHEN Juan
    Subtropical Plant Science    2024, 53 (5): 468-470.   DOI: 10.3969/j.issn.1009-7791.2024.05.010
    Abstract73)      PDF(pc) (1104KB)(21)       Save
    The article offers additional floral morphology of a Costaceae species recently described from Yunnan, namely Parahellenia malipoensis Juan Chen, L.Y. Zeng, S. Jin Zeng & N. H. Xia. This species is readily distinguished from other Parahellenia species by distinctive morphological characters, such as very small stature, unbranched stems, small and plicate leaf blades, small inflorescences and bract with a soft spine at apex, small flowers, and notably short yellow anther crest ornamented with dark red lines.
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    Introduction of Nomenclature Section of ⅩⅩ International Botanical Congress
    MA Jin-shuang
    Subtropical Plant Science    2024, 53 (5): 487-494.  
    Abstract54)      PDF(pc) (1082KB)(20)       Save
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